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    Review: Long-term research activity on the biostimulant properties of natural origin compounds

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    For ten years the Department of Agricultural Biotechnology, University of Padua, supported by the company ILSA SpA, Arzignano, (VI), Italy, has been studying, via different methodologies, the biostimulant properties of products derived from different organic raw materials. The research activity includes the development of specific bioassays designed for understanding the biological activity of the various compounds and the evaluation of their effects on maize metabolism, grown under controlled conditions. Plants treated with these products confirmed their biological activity in terms of capacity to increase plant growth and stimulate plant nitrogen and carbon metabolisms. In addition, the effects of phenol-containing organic substances derived from agro-industrial byproducts were assayed on phenylpropanoid metabolism. Phenol-containing products, in particular, increased plant antioxidants, key elements for human health. The products also increased plant biomass, exerted a positive role on nitrogen metabolism and stimulated the activity and gene expression of phenylalanine ammonia-lyase, the first enzyme of the phenylpropanoid pathway. Our results indicate that biostimulants can be used as bioactive products in agriculture to increase plant yield and resistance to stress conditions

    Biostimulant activity of two protein hydrolysate in peroxidase and esterase activity of maize seedlings.

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    Two protein-hydrolysate-based fertilizers (PHFs), one from legume (LH) and one from the mixture of Spirulina spp. and alfalfa (SA), were studied chemically and biologically. LH and SA showed a different chemical composition and different degree of hydrolysis. The biostimulant activity was investigated using two specific and sensitive bioassays of auxins (IAA) and gibberellins (GA). Extracts of LH and SA elicited an auxin and gibberellin-like activity. To improve our understanding on the biostimulant activity of PHFs, LH and SA were supplied to maize plants and their effect on growth, chlorophyll and sucrose content was studied. Besides, the effect of PHFs was tested on peroxidase and esterase activity, enzymes notoriously used as markers in growth, differentiation and organogenesis- related processes. The biostimulant products increased leaf growth and induced morphological changes in root architecture similarly to growth factors. Therefore, it may be supposed that PHFs and IAA and GA can share the same properties. LH and SA positively affected peroxidase and esterase activities, suggesting their important role in the induction of growth and cell differentiation. Moreover, the addition of PHFs to plants increased chlorophyll content and decreased the level of sugars, indicating a positive influence of the PHFs on the photosynthetic process
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