8 research outputs found
Scutisotoma indigirka Potapov & Babenko & Fjellberg 2006, sp.n.
No full textScutisotoma indigirka sp.n. Figs. 139–142 Material. Holotype: ♀, Russia, Yakutia, Oimyakon Province, middle part of Indigirka river, near Ust’-Nera village. (64 o 33’N, 143 o 13’E), 1600 m alt., northern slope, mosses and lichen on stones, 22.vii.1992, leg. M. Potapov. Paratypes: 15 exx., same sample (MSPU). Description. Size 0.6–0.9 mm. Colour rather light, yellowish-brown, eye spots and apical part of antennae darker. Cuticle with fine primary granulation. Ocelli 8+8, G and H smaller. PAO elliptical, rather wide and not constricted, about 2.0–2.5 as long as ocellus diameter and 0.9–1.3 as long as U 3. Maxillary outer lobe with 4 sublobal hairs and simple maxillary palp. Labral formula 3/554. Labium with all 5 papillae present, papilla E without guard e 7. Proximal field with 3 chaetae, basomedian field with 4. Ventral side of a head with 3+3 postlabial chaetae. Ant.1 with 2 basal microsensilla (bms, dorsal and ventral), and 2 ventral sensilla (s). Ant.2 with 3 bms and 1 laterodistal s, Ant.3 with 1 bms and 5 distal s (AO and one lateral s). Guard sensilla of AO clearly larger than inner pair of sensilla. Additional sensilla on Ant. 2–3 in males not observed. Sensilla on Ant.4 hardly differentiated, subapical organite small. Dorsal axial chaetom of Th.II–Abd.III as 6–7,6/334. Thorax without ventral axial chaetae. Macrochaetae differentiated only laterally on Th.II–III and on tip of abdomen. Medial macrochaetae on Abd.V are about 0.4 as long as tergite. Sensilla on tergites clearly differentiated, shorter than ordinary chaetae. Sensillary formula 33/22224 (s) and 11/111 (ms). Sensilla on Abd.I–III in mid-tergal position (medial sensillum on Abd.III in posterior 1/3 of the tergite). Microsensillum on Abd.III set close to the lateral sensillum (Fig. 141). On Abd.V the lateral pair of sensilla (accp3 and accp4) clearly thicker and one of them moved anteriorly (Fig. 142). Unguis simple without inner tooth. Ti.1–3 usually with the basic set of chaetae (21-21- 25). B-row on Ti.1–2 with 7 chaetae. Chaetae x and B 5 on Ti. 3 in males hardly different from other chaetae. Tibiotarsal tenent chaetae (1-2-2) clearly clavate, longer than unguis (U 3: t.ch. = 0.7–1.0: 1). Ventral tube with 4+4 latero-distal and usually with 4 posterior chaetae. Tenaculum with 1–3 chaetae and 4+4 teeth (sometimes one tooth may be absent on one side). Anterior furcal subcoxa with 8–10 chaetae, posterior with 5–6 chaetae. Furca rather short. Anterior side of manubrium with a pair of distal chaetae, posterior side with 11–13+11–13 (including 3+3 on laterobasal lobes). Dens distally with one anterior chaeta, posterior side almost smooth, with 3–4 chaetae (Figs. 139, 140). Retinacular ridges of dens strong. Mucro principally as in S. subarctica (Gisin), rather massive, with 3 teeth, lamellae absent (Fig. 107). Ratio of manubrium: dens: mucro = 3.2–4.7: 1.4–2.2: 1. Lateral anal lobes each with two relatively long and one small chaetae, upper anal lobe with two small and one long chaetae. Affinity. The new species is obviously closely related to S. subarctica (Gisin). Both species are separated from other members of the genus by some unique characters (simple maxillary palp, 3 prelabral chaetae, reduced number of guards on labial papilla E), and their generic position may be changed in future. The two species differ in chaetotaxy of dens (6 anterior, 7–8 posterior in subarctica, 1 anterior, 3–4 posterior in indigirka) and in the number of laterodistal chaetae on VT (3+ 3 in subarctica, 4+ 4 in indigirka). S. indigirka has the largest manubrium: dens ratio (2.3 in average) of all Scutisotoma species studied here and generally the furca of the new species is rather similar to that of Weberacantha and Narynia. Distribution. Known only from the type-locality in Yakutia. Name derivation. The new species is named after the type-locality.Published as part of Potapov, Mikhail, Babenko, Anatoly & Fjellberg, Arne, 2006, Taxonomy of the Proisotoma complex. Redefinition of genera and description of new species of Scutisotoma and Weberacantha (Collembola, Isotomidae), pp. 1-74 in Zootaxa 1382 (1) on pages 51-52, DOI: 10.11646/zootaxa.1382.1.1, http://zenodo.org/record/507146
Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy
No full textInducible chemical-genetic fluorescent
markers are promising tools
for live cell imaging requiring high spatiotemporal resolution and
low background fluorescence. The fluorescence-activating and absorption
shifting tag (FAST) was recently developed to form fluorescent molecular
complexes with a family of small, synthetic fluorogenic chromophores
(so-called fluorogens). Here, we use rational design to modify the
binding pocket of the protein and screen for improved fluorescence
performances with four different fluorogens. The introduction of a
single mutation results in improvements in both quantum yield and
dissociation constant with nearly all fluorogens tested. Our improved
FAST (iFAST) allowed the generation of a tandem iFAST (td-iFAST) that
forms green and red fluorescent reporters 1.6-fold and 2-fold brighter
than EGFP and mCherry, respectively, while having a comparable size
Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors
No full textFluorescent reporters
are essential components for the design of
optical biosensors that are able to image intracellular analytes in
living cells. Herein, we describe the development of circularly permuted
variants of Fluorescence-Activating and absorption-Shifting Tag (FAST)
and demonstrate their potential as reporting module in biosensors.
Circularly permutated FAST (cpFAST) variants allow one to condition
the binding and activation of a fluorogenic ligand (and thus fluorescence)
to analyte recognition by coupling them with analyte-binding domains.
We demonstrated their use for biosensor design by generating multicolor
plug-and-play fluorogenic biosensors for imaging the intracellular
levels of Ca2+ in living mammalian cells in real time
Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors
No full textFluorescent reporters
are essential components for the design of
optical biosensors that are able to image intracellular analytes in
living cells. Herein, we describe the development of circularly permuted
variants of Fluorescence-Activating and absorption-Shifting Tag (FAST)
and demonstrate their potential as reporting module in biosensors.
Circularly permutated FAST (cpFAST) variants allow one to condition
the binding and activation of a fluorogenic ligand (and thus fluorescence)
to analyte recognition by coupling them with analyte-binding domains.
We demonstrated their use for biosensor design by generating multicolor
plug-and-play fluorogenic biosensors for imaging the intracellular
levels of Ca2+ in living mammalian cells in real time
Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors
No full textFluorescent reporters
are essential components for the design of
optical biosensors that are able to image intracellular analytes in
living cells. Herein, we describe the development of circularly permuted
variants of Fluorescence-Activating and absorption-Shifting Tag (FAST)
and demonstrate their potential as reporting module in biosensors.
Circularly permutated FAST (cpFAST) variants allow one to condition
the binding and activation of a fluorogenic ligand (and thus fluorescence)
to analyte recognition by coupling them with analyte-binding domains.
We demonstrated their use for biosensor design by generating multicolor
plug-and-play fluorogenic biosensors for imaging the intracellular
levels of Ca2+ in living mammalian cells in real time
Development of a Rubredoxin-Type Center Embedded in a <i>de Dovo</i>-Designed Three-Helix Bundle
No full textProtein design is
a powerful tool for interrogating the basic requirements
for the function of a metal site in a way that allows for the selective
incorporation of elements that are important for function. Rubredoxins
are small electron transfer proteins with a reduction potential centered
near 0 mV (vs normal hydrogen electrode). All previous attempts to
design a rubredoxin site have focused on incorporating the canonical
CXXC motifs in addition to reproducing the peptide fold or using flexible
loop regions to define the morphology of the site. We have produced
a rubredoxin site in an utterly different fold, a three-helix bundle.
The spectra of this construct mimic the ultraviolet–visible,
Mössbauer, electron paramagnetic resonance, and magnetic circular
dichroism spectra of native rubredoxin. Furthermore, the measured
reduction potential suggests that this rubredoxin analogue could function
similarly. Thus, we have shown that an α-helical scaffold sustains
a rubredoxin site that can cycle with the desired potential between
the Fe(II) and Fe(III) states and reproduces the spectroscopic characteristics
of this electron transport protein without requiring the classic rubredoxin
protein fold
Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors
No full textFluorescent reporters
are essential components for the design of
optical biosensors that are able to image intracellular analytes in
living cells. Herein, we describe the development of circularly permuted
variants of Fluorescence-Activating and absorption-Shifting Tag (FAST)
and demonstrate their potential as reporting module in biosensors.
Circularly permutated FAST (cpFAST) variants allow one to condition
the binding and activation of a fluorogenic ligand (and thus fluorescence)
to analyte recognition by coupling them with analyte-binding domains.
We demonstrated their use for biosensor design by generating multicolor
plug-and-play fluorogenic biosensors for imaging the intracellular
levels of Ca2+ in living mammalian cells in real time
