41 research outputs found
Terapia e profilassi in avicoltura: l’evoluzione dell’antibiotico-resistenza di E.coli e Salmonelle dal 1989 al 1998
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ELISA antigen capture test for detecting Chlamydia spp. in cloacal swabs obtained from birds of prey.
No full textDuring 1995-96 we were carried out an investigation about the presence of Chlamydia spp. in birds of prey stayed in W.W.F. Rehabilitation Centers in Campania region (Italy). Were checked 14 species of raptors (6 accipitridae, 3 falconidae, 5 strigidae) and the total number of 51 cloacal swabs was obtained for three days on end. Were searched the presence of the specific group antigen of Chlamydia spp. by ELISA Ideia Chlamydia Test (Dako), diagnostic quick micromethod test formulated for human medicine, but employed also in veterinary field, particularly in ovine and avian fields. Chlamydial infection was shown in two subjects of Marsh harriers (Circus aeruginosus) without any clinical signs related to Chlamydiosis. The AA. emphasized the risk of trasmission of such zoonosis to operators of Rehabilitation Centers through the contact with carrier birds of infection
L’andamento dal 1989 al 1997 dell’antibiotico resistenza di E.coli e Salmonelle. Considerazioni sulle misure di terapia e profilassi
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Prove in vitro del potere inattivante dell’ozono verso enterobatteri patogeni di origine aviare
No full textAltered folate receptor 2 expression in uraemic patients on haemodialysis: Implications for folate resistance
No full textBackground. Folate therapy reduces, but does not normalize homocysteine (Hcy) levels, frequently elevated in chronic kidney disease (CKD). The mechanisms of this folate resistance are unknown. Cellular acquisition of folate is mediated by folate receptors (FRs), whose expression is also modulated by folate status, through an Hcy-dependent regulation mechanism involving heterogeneous nuclear ribonucleoprotein-E1 (hnRNP-E1). Our objective was to evaluate whether an alteration of the FR2 (the form present in nucleated blood cells) expression is present in CKD patients on hemodialysis (HD), and its susceptibility to folate treatment.
Methods. A population of chronic uremic patients on HD was enrolled, along with a control group, and studies on FR2 receptor expression and related items were performed in plasma and mononuclear cells from peripheral blood. A subgroup of patients was treated with ev methyltetrahydrofolate for one month.
Results. In HD, there was a significant reduction in FR2 protein expression compared to controls, not correlated with Hcy concentrations, while its mRNA levels were significantly increased. After folate treatment, there was a significant mRNA decrease, in the absence of significant changes in receptor protein expression. hnRNP-E1 gene and protein expression levels increased pre-treatment, while decreased post-treatment.
Conclusions. In HD, FR2 expression is altered in peripheral mononuclear cells, since its levels are decreased and are not responsive to variations in Hcy concentration, while the intracellular machinery (receptor mRNA and hnRNP-E1), possibly triggering its regulation, is conserved. These findings provide insight into the mechanisms of folate resistance in uremia.Background Folate therapy reduces, but does not normalize homocysteine (Hcy) levels, frequently elevated in chronic kidney disease (CKD). The mechanisms of this folate resistance are unknown. Cellular acquisition of folate is mediated by folate receptors (FRs), whose expression is also modulated by folate status, through an Hcy-dependent regulation mechanism involving heterogeneous nuclear ribonucleoprotein-E1 (hnRNP-E1). Our objective was to evaluate whether an alteration of the FR2 (the form present in nucleated blood cells) expression is present in CKD patients on haemodialysis (HD), and its susceptibility to folate treatment. Methods A population of chronic uraemic patients on HD was enrolled, along with a control group, and studies on FR2 receptor expression and related items were performed in plasma and mononuclear cells from peripheral blood. A subgroup of patients was treated with methyltetrahydrofolate for 1 month.ResultsIn HD, there was a significant reduction in FR2 protein expression compared with controls, not correlated with Hcy concentrations, while its mRNA levels were significantly increased. After folate treatment, there was a significant mRNA decrease, in the absence of significant changes in receptor protein expression. hnRNP-E1 gene and protein expression levels increased pre-treatment, while decreased post-treatment. Conclusions In HD, FR2 expression is altered in peripheral mononuclear cells, since its levels are decreased and are not responsive to variations in Hcy concentration, while the intracellular machinery (receptor mRNA and hnRNP-E1), possibly triggering its regulation, is conserved. These findings provide insight into the mechanisms of folate resistance in uraemia. © 2013 © The Author 2013. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved
Analisi molecolare di ceppi del Circovirus della Malattia del Becco e delle Penne degli Psittacidi in Italia. Risultati preliminari
No full textPsittacine Beak and Feather Disease (PBFD) shows a broad host range within captive psittacine populations. Since clinical findings of PBFD in parrots often differ on the basis of the species and the age of infected psittacines, it has been speculated that a species-specificity of PBFDV strains may exists. The phylogenetic analysis of 9 BBFDV strains isolated in Italy from October 2010 to February 2011 showed some degree of genetic variability related to the infected species and clinical signs
Isolation of a influenza virus strain type A, subtype H3N2 from broilers of probably swine derive
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