480 research outputs found
A conglomerate, by Harvey H. Daugherty; with a sketch of the author's life.
No full text6 p. l., 422 p
Rapid dilation of the abdominal aorta during infusion of angiotensin II detected by noninvasive high-frequency ultrasonography
No full textBackgroundInfusion of angiotensin II (AngII) via subcutaneous osmotic pumps into mice promotes the development of abdominal aortic aneurysms (AAAs). These AngII-induced AAAs develop via a complex process in which there is a transmedial break, lumen dilation, thrombus formation, inflammation involving cells of both the innate and acquired immune systems, and remodeling. The recent development of a high-frequency ultrasound machine has permitted the noninvasive detection of murine abdominal aortas. We assessed the ability of a Visualsonics Vevo 660 high-resolution imaging system to detect AAAs and sequentially quantify the aortic luminal diameter. This system had 100% accuracy in detecting AngII-induced AAAs in vivo, with intrauser and interuser variation coefficients of less than 10% for quantification of the aortic lumen diameter.MethodsMale apolipoprotein E (apoE)−/− mice were infused subcutaneously with either saline or AngII and were monitored with this ultrasonic system to define the temporal changes in aortic lumen diameter. Aortic luminal diameters were measured in the aneurysm-susceptible region of the suprarenal aorta. For internal controls, abdominal aortic diameters were measured at the level of the left renal branch, because this landmark region did not dilate during AngII infusion.ResultsLuminal diameters of the suprarenal aorta did not change significantly in saline-infused mice over 28 days of measurement (P = .71). In contrast, AngII infusion led to rapid dilation of suprarenal aortas during the initial 7 days of infusion (0.071 mm/d; P = .0037 for the change in the initial expansion rate). Further luminal diameter expansions occurred for the remaining 21 days of observation at a more modest rate (0.023 mm/d; P = .0001 for continued expansion after day 7). Within the initial 14 days of AngII infusion, some apoE−/− mice died as a result of rupture of the aorta in the suprarenal region. We had previously assumed that aortic dilation and rupture occurred simultaneously. However, in the AngII-infused mice that succumbed to aortic rupture, luminal diameters increased several days before death.ConclusionsHigh-frequency ultrasonography demonstrated that suprarenal aortic expansion occurs rapidly after the initiation of AngII infusion into apoE−/− mice.Clinical RelevanceAngiotensin II has been inferred to have an important role in the development of human aortic diseases. Infusion of angiotensin II into mice leads to the development of abdominal aortic aneurysms. Definition of the natural history of abdominal aortic aneurysm development in animal models of the disease may provide insight into the factors associated with initiation and propagation in the human disease. The recent development of high-frequency ultrasonography has permitted the sequential noninvasive detection of mouse aortic luminal dimensions during angiotensin II infusion. The convergence of studies on aortic dimensions, in association with pathologic characterization of the tissue, should provide a means to define mechanisms of abdominal aortic aneurysm formation
Public Health Aspects of the Daugherty Water for Food Institute Strategic Plan
No full textThe University of Nebraska College of Public Health is committed to contributing to the research, service and education programs of the Daugherty Water for Food Institute. The College can offer a wide variety of expertise that can contribute to programs related to water, food and health. This document has been written to highlight the areas in which College faculty have particular interests, knowledge and experience to contribute.https://digitalcommons.unmc.edu/coph_reports/1000/thumbnail.jp
Non-consumptive ocean recreation in Oregon: human uses, economic impacts and spatial data
No full textsubmitted under a joint effort of the Surfrider Foundation, NaturalEquity, and Ecotrust ; Chris LaFranchi, Collin Daugherty, NaturalEquity.Title from PDF cover (viewed on May 6, 2020).This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (page 31).Funding was provided by the David and Lucile Packard Foundation.Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Dissecting Class A Scavenger Receptor Mediated Cell Adhesion and Lipoprotein Internalization
No full textMacrophage Class A scavenger receptors (SR-A) are trimeric transmembrane glycoproteins that can bind a variety of ligands including modified lipoproteins and bacterial products. Through its ability to internalize these ligands, SR-A is thought to be involved in many physiological and pathophysiological processes such as host defense and atherosclerosis. In vitro, SR-A also mediates cell adhesion to modified extracellular matrix proteins. However, the physiological role of SR-A mediated cell adhesion is unknown. The goal of this project was to dissect SR-A mediated cell adhesion and ligand internalization through structure-function studies and to develop a mutant of SR-A that specifically lacks the adhesive properties. It appeared that SR-A mediated adhesion and ligand internalization depend on the same extracellular domain. Therefore, I investigated the role of cytoplasmic domains for SR-A mediated adhesion. Different cytoplasmic mutated SR-A constructs were stably expressed in human embryonic kidney (HEK-293) cells. I found that a mutated SR-A deficient in all but the six amino acids proximal to the membrane of the cytoplasmic tail (SR-AΔ1-49) was able to mediate cell adhesion, but not receptor internalization. Substitution of the SR-A cytoplasmic tail with that of the transferrin receptor resulted in retention of this chimeric receptor in the endoplasmic reticulum demonstrating that the SR-A membrane proximal amino acids are also critical for transport of the receptor from the endoplasmic reticulum to the Golgi apparatus. In summary, my results demonstrate that SR-A-mediated adhesion and internalization require distinct cytoplasmic domains and I developed a mutant SR-A (SR-AΔ1-49) that specifically meditates cell adhesion. This mutant will make it possible to specifically address the physiological role of SR-A mediated adhesion.Die Trennung der Adhäsionseigenschaften von den Internalisationseigenschaften des Scavenger Rezeptors der Klasse A Die Makrophagen Scavenger-Rezeptoren der Klasse A (SR-A) sind trimere integrale Transmembranproteine, die eine Vielfalt von Liganden (z.B. modifizierte Lipoproteine und bakterielle Produkte) binden. Durch Internalisation dieser Liganden spielt SR-A eine wichtige Rolle in verschiedenen physiologischen und pathophysiologischen Vorgängen, unter anderem im Abwehrsystem und bei der Pathogenese der Arteriosklerose. In vitro kann SR-A auch die Adhäsion von Makrophagen zu modifizierten extrazellulären Matrixproteinen regulieren, die physiologische Bedeutung dieser Adhäsionseigenschaften ist allerdings noch unklar. Das Ziel dieser Arbeit war es, die Adhäsionseigenschaften von den Internalisationeigenschaften des Rezeptors zu trennen und einen mutierten SR-A zu entwickeln, dem die Adhäsionseingenschaften fehlen. Da Zelladhäsion und Internalisation von Liganden anscheinend von einer identischen extrazellulären Region des Rezeptors reguliert werden, habe ich die zytoplasmatische Region des Rezeptors erforscht. Veschiedene mutierte SR-A Konstrukte wurden in HEK-293 (human embryonic kidney) Zellen exprimiert. Mit diesem Ansatz konnte gezeigt werden, dass ein mutierter SR-A (SR-AΔ1-49) mit einer verkürzten zytoplasmatischen Region von nur sechs Aminosäuren Zelladhäsion regulieren kann, aber nicht in der Lage ist, die Internalisation des Rezeptors zu regulieren. Studien mit einem chimären Rezeptor, in den der zytoplasmatische Teil von SR-A durch den des Transferrin Rezeptors ersetzt wurde, haben gezeigt, dass diese zytoplasmatische SR-A Region auch für den Transport vom Endoplasmatischen Reticulum zum Golgi Apparat nötig ist. Es konnte gezeigt werden, dass Zelladhäsion und Internalisation von unterschiedlichen zytoplasmatischen Regionen reguliert werden. Es wurde ein mutierter SR-A entwickelt, der spezifisch die Zelladhäsion reguliert und anhand dieses Rezeptors kann die physiologische Bedeutung der Adhäsionseigenschaften untersucht werden
PERCEPTIONS OF INDIANA’S ENGINEERING/TECHNOLOGY EDUCATION CLASSROOM TEACHERS AS MEASURED BY THE CHARACTERISTICS OF TECHNOLOGY EDUCATION SURVEY
No full textThe purpose of this study was to determine Engineering/Technology Education (ETE) teachers’ perceptions of Project Lead The Way’s (PLTW) pre-engineering program in the state of Indiana utilizing the Characteristics of Technology Education Survey (CTES) (Daugherty, Hill, & Wicklein, 1996). The study focused on the perceptions of teachers who were and were not teaching PLTW’s pre-engineering curriculum as they related to curriculum content, teaching methodology, curriculum integration, and fit of curriculum in school environment. Two hundred and eighty two or 51.3% of Indiana high school ETE teachers responded to the 46 question CTES. Analysis of variance (ANOVA) and multivariate analysis of variance (MANOVA) were conducted to test for significance. The study found no significant differences in the perceptions of ETE teachers who were and who were not teaching PLTW’s pre-engineering program
Birch Tree Apartments and wrap-around services
No full textThe Birch Tree Affordable Housing Project is located in the rural Southeast Missouri community of Farmington. The Project involves the complete rehabilitation of a 56-unit apartment complex and a set of complementary wraparound services. The wrap-around services include a Head Start Center located within the complex itself, a community garden, Financial and Job Search Life Skills programs, Assets for Independence and Family Self-Sufficiency programs, a Summer Food feeding site, and a Volunteer Income Tax Assistance program. Given current community dynamics, housing affordability is a difficult matter for many Farmington residents. Specifically, approximately 37% of the households in Farmington spend 58% of their income on housing and are unable to pay for food, utilities, clothing, or save for an emergency. Should this persist unabated, Farmington risks increased poverty, possible home loss, and increased social and economic burden to the community. The Birch Tree Affordable Housing Project will allow families to increase their budgeting skills to plan for the financial resources that are available. It will also increase affordable and quality child care; increase the amount of safe, sanitary, decent, and affordable housing; increase the number of economically disadvantaged people getting education and training required for better paying jobs; and increase the income for economically disadvantaged families. (Author Abstract)Daugherty, K. L. (2012). Birch Tree Apartments and wrap-around services. Retrieved from http://academicarchive.snhu.eduMaster of Science (M.S.)Community Economic DevelopmentSchool of Busines
Executive Secrets: Covert Action and the Presidency
No full textA frank and refreshing evaluation of several Chief Executives, their Directors of Central Intelligence, and even some lower in the hierarchy, Executive Secrets shines light on the development and execution of foreign policy through the understanding of the tools available, of which covert action may be least known and understood. This book is a great tool for the press, the public, and many political appointees in the National Security System. A History Book Club Selection with a foreword by Mark Bowden, author of Black Hawk Down.
[Daugherty] gives a frank and refreshing evaluation of several Chief Executives, their Directors of Central Intelligence, and even some lover in the hierarchy. Anyone interested or involved in the development and execution of foreign policy must know the tools available, of which covert action may be least known and understood. This understanding is also pertinent to a wider audience: the press, the public, and many political appointees in the National Security system. This book should help. -- Air Power History
A most informative study of covert action.... This well-researched, thoughtful book is a valuable work that will be treasured by scholars and professionals in the field. Highly recommended. -- Choice
A hard-hitting, balanced and highly successful effort to deal with the issue of presidential responsibility for covert action. -- John Stempel, Patterson School of Diplomacy, University of Kentucky
Daugherty has done us all a tremendous service by attempting to rescue the Agency from the myths, both well-meaning and malevolent, that shape our understanding of it. . . . This book ought to dispel some of the fog that obscures our understanding of the C.I.A. and that prompts the gigantic mood swings in our attitude toward intelligence gathering and covert actions. -- Mark Bowden, from the foreword
There are some interesting and carefully drawn vignettes of covert operations here that demonstrate a skillful blending of declassified documents, investigative reports, and secondary sources. It is these case studies that make this one of those essential works for specialists in intelligence policy. -- Register of the Kentucky Historical Society
If you are a keen observer of the agency, this one will interest and instruct you. -- Rockland (ME) Courier-Gazette
Offers a richly textured book that clears up some common misconceptions of the CIA, as well as an insider\u27s perspective on how covert action really works, or fails. -- Savannah (GA) Morning News
Readers of this timely and occasionally revelatory account may be surprised to learn that President Carter approved a covert action program to attack the internal legitimacy of the Soviet system, and that he repeatedly relied on covert action to achieve his foreign policy aims. -- Zbigniew Brzezinski
Named a 2005 Choice Outstanding Academic Title.
[Daugherty] has done a great service for those wanting to seriously understand the realistic capabilities of the organization. This is not an easy task, for the CIA has been the subject of many books, movies, and certain mindsets in the population. --Foreign Area Officer Journalhttps://uknowledge.uky.edu/upk_political_science_american_politics/1038/thumbnail.jp
Does changing the name of technology education to technology and engineering increase the program's prestige
No full textIncludes bibliographical references
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