106 research outputs found
Simultaneous determination of cinnarizine and domperidone in marketed tablets using a green HPTLC method
No full textQuantitative estimation of hesperidin by HPTLC in different varieties of citrus peels
No full textABSTRACTObjectiveTo develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.MethodsThe method was carried out in aluminum-backed silica gel 60 F254 plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.ResultsA compact band was obtained for hesperidin at Rf value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.9986 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.ConclusionsThe method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient
Stability-indicating densitometric HPTLC method for qualitative and quantitative analysis of arbutin in commercial whitening creams
No full textA Simple, Cost-Effective, and Green HPTLC Method for the Estimation of Ascorbic Acid in Solvent and Ultrasound-Assisted Extracts of Phyllanthus emblica, Capsicum annuum, and Psidium guajava
No full textGreener analytical methodologies for the estimation of ascorbic acid (AA) are poorly reported in the literature. Furthermore, the green indexes of the literature’s analytical assays of AA estimation have not been assessed. As a consequence, the aim of this research is to invent and validate a simple, cost-effective, and green reverse-phase “high-performance thin-layer chromatography (HPTLC)” method for the estimating AA in the solvent extracts (SE) and ultrasound-assisted extracts (UAE) of Phyllanthus emblica, Psidium guajava, and Capsicum annuum. The greener mobile phase for AA estimation was a binary mixture of water and ethanol (70:30, v/v). At a wavelength of 265 nm, the detection of AA was carried out. The greener HPTLC technique was linear in the 25–1200 ng/band range. In addition, the method was simple, cost-effective, accurate, precise, robust, sensitive, and green. The amount of AA was highest in the SE and UAE of P. emblica compared to the SE and UAE of P. guajava and C. annuum. The amount of AA in the SE of P. emblica, P. guajava, and C. annuum was found to be 491.16, 168.91, and 144.30 mg/100 g, respectively. How-ever, the amount of AA in the UAE of P. emblica, P. guajava, and C. annuum was found to be 673.02, 218.71, and 199.30 mg/100 g, respectively. Using the “analytical GREEnness (AGREE)” methodology, the greenness index for the developed method was calculated to be 0.88, showing that the developed method has an excellent green profile. When it came to extracting AA, the UAE method outperformed the SE method. These findings suggested that the developed method might be used to estimate the AA in a variety of vegetable crops, plant-based extracts, and commercial formulations. Furthermore, because of the use of greener solvent systems against the commonly utilized hazardous solvent systems for AA determination, this technique is also safe and sustainable
A Selective and Accurate LC-MS/MS Method for Simultaneous Quantification of Valsartan and Hydrochlorothiazide in Human Plasma
No full textThe fixed dose combination of valsartan (VAL) and hydrochlorothiazide (HCTZ) is the most commonly prescribed medicine for the effective treatment of hypertension. In this study, a simple sensitive and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous quantitation of VAL and HCTZ in human plasma by using irbesartan (IRB) and hydroflumethiazide (HFMZ) as their specific internal standards (ISs). HLB cartridge-based solid-phase extraction was used for the extraction of analytes and ISs. The chromatographic separation was achieved on Lichrocart RP Select (125 × 4 mm), 5 nm with the mobile phase composition of acetonitrile: 10 mM ammonium acetate buffer: 95:05, v/v, at flow rate of 0.5 mL/min. The turbo ion electrospray ionization in negative mode was used as ion source for the sample ionization. The precursor to product ion transitions were 434.10 > 179.10 (VAL), 295.70 > 204.90 (HCTZ), 427.10 > 192.90 (IRB), and 329.90 > 302.40 (HFMZ) for detection and quantification of analytes and their ISs. The retention times of VAL and HCTZ were 1.90 min and 2.30 min, respectively. The range for the calibration curves of VAL and HCTZ were 50.2–6018.6 ng/mL and 1.25–507.63 ng/mL, respectively, with good linearity having correlation coefficient values of ≥0.995 for both VAL and HCTZ. All validation parameter results (selectivity, precision and accuracy, matrix effects and stabilities) were within the acceptable range as per USFDA guideline for bioanalytical method validation. The intra-day and inter-day accuracy data for VAL were within the range of 105.68–114.22% and 98.41–108.16%, respectively, whereas for HCTZ they were 87.01–101.18% and 95.16–99.37%, respectively. The ion suppression effects produced for VAL and ion enhancement effects produced for HCTZ were insignificant according to the proposed sample cleanup procedure. The developed LC-MS/MS method was successfully applied to bioequivalence study on healthy volunteers
HiGh-performance thin-layer chromatography method for the simultaneous determination of vulgarin and epivulgarin biomarkers in <i>Artemisia judaica</i> L.
No full textDensitometric-high-performance thin-layer chromatographic estimation of diosmin, hesperidin, and ascorbic acid in pharmaceutical formulations
No full textSolubility of Cinnarizine in (Transcutol + Water) Mixtures: Determination, Hansen Solubility Parameters, Correlation, and Thermodynamics
No full textBetween 293.2 and 313.2 K and at 0.1 MPa, the solubility of the weak base, cinnarizine (CNZ) (3), in various {Transcutol-P (TP) (1) + water (2)} combinations is reported. The Hansen solubility parameters (HSP) of CNZ and various {(TP) (1) + water (2)} mixtures free of CNZ were also predicted using HSPiP software. Five distinct cosolvency-based mathematical models were used to link the experimentally determined solubility data of CNZ. The solubility of CNZ in mole fraction was increased with elevated temperature and TP mass fraction in {(TP) (1) + water (2)} combinations. The maximum solubility of CNZ in mole fraction was achieved in neat TP (5.83 × 10−2 at 313.2 K) followed by the minimum in neat water (3.91 × 10−8 at 293.2 K). The values of mean percent deviation (MPD) were estimated as 2.27%, 5.15%, 27.76%, 1.24% and 1.52% for the “Apelblat, van’t Hoff, Yalkowsky–Roseman, Jouyban–Acree, and Jouyban–Acree–van’t Hoff models”, respectively, indicating good correlations. The HSP value of CNZ was closed with that of neat TP, suggesting the maximum solubilization of CNZ in TP compared with neat water and other aqueous mixtures of TP and water. The outcomes of the apparent thermodynamic analysis revealed that CNZ dissolution was endothermic and entropy-driven in all of the {(TP) (1) + water (2)} systems investigated. For {(TP) (1) + water (2)} mixtures, the enthalpy-driven mechanism was determined to be the driven mechanism for CNZ solvation. TP has great potential for solubilizing the weak base, CNZ, in water, as demonstrated by these results
Estimation of Swertiamarin in Enicostemma Littorale and Marketed Formulations Using HPLC-UV Method
No full textInternational audienceA simple, economic, reproducible, robust and precise HPLC method for estimation of swertiamarin in both 60 % methanolic extract of Enicostemma littorale and marketed formulations was developed and validated in present investigation. The mobile phase composed of methanol and water (90:10 % v/v), gave a sharp and well-defined peak of swertiamarin at the retention time of 10.15 ± 1.52 min. The limit of detection (LOD) and limit of quantification (LOQ) were 17.25 and 56.92 µg/ml respectively. The proposed method with high degree of precision and accuracy was employed for the estimation of swertiamarin in methanolic extract and in marketed formulation
Simultaneous Determination of Menthol and Eucalyptol by the Densitometric HPTLC Method in Some External Analgesic Formulations
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