78 research outputs found
El idioma del mundo. Sobre David Lorente Fernández, El cuerpo, el alma, la palabra. Medicina nahua en la Sierra de Texcoco
No full textThis review by Pedro Pitarch discusses David Lorente Fernández’s book on Nahua medicine in the Sierra de Texcoco. Drawing from an ethnographic perspective, the author explores how illness and healing serve as a language to understand social, political, and spiritual relationships in peri-urban Indigenous communities. The review emphasizes the book’s innovative focus on domestic and maternal medicine, and the interplay between traditional knowledge and biomedicine, highlighting the persistence of an Indigenous cosmopolitics that interprets the world through the body and illnessEsta reseña, escrita por Pedro Pitarch, analiza el libro de David Lorente Fernández sobre la medicina nahua en la Sierra de Texcoco. A partir de una perspectiva etnográfica, el autor examina cómo las enfermedades y sus tratamientos constituyen un lenguaje para comprender las relaciones sociales, políticas y espirituales en comunidades indígenas periurbanas. La reseña destaca la originalidad del enfoque en la medicina doméstica femenina y la interacción entre saberes tradicionales y biomedicina, subrayando la persistencia de una cosmopolítica indígena que interpreta el mundo a través del cuerpo y la enfermedadInstituto de Investigaciones Históricas, Universidad Nacional Autónoma de Méxic
Top-down characterization data on the speciation of the Candida albicans immunome in candidemia
No full textAbstractThe characterization of pathogen-specific antigenic proteins at the protein species level is crucial in the development and molecular optimization of novel immunodiagnostics, vaccines or immunotherapeutics for infectious diseases. The major requirements to achieve this molecular level are to obtain 100% sequence coverage and identify all post-translational modifications of each antigenic protein species. In this article, we show nearly complete sequence information for five discrete antigenic species of Candida albicans Tdh3 (glyceraldehyde-3-phosphate dehydrogenase), which have been reported to be differentially recognized both among candidemia patients and between candidemia and control patients. A comprehensive description of the top-down immunoproteomic strategy used for seroprofiling at the C. albicans protein species level in candidemia as well as for the chemical characterization of this immunogenic protein (based on high-resolution 2-DE, Western blotting, peptide mass fingerprinting, tandem mass spectrometry and de novo peptide sequencing) is also provided. The top-down characterization data on the speciation of the C. albicans immunome in candidemia presented here are related to our research article entitled “Seroprofiling at the Candida albicans protein species level unveils an accurate molecular discriminator for candidemia” (Pitarch et al., J. Proteomics, 2015, http://dx.doi.org/10.1016/j.jprot.2015.10.022)
Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the <i>Candida albicans</i> Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis
No full textInvasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters is a predisposing factor for IC. Hyphal forms of Candida albicans (the major etiological agent of IC) are related to invasion of host tissues. The secreted proteins of hyphae are involved in virulence, host interaction, immune response, and immune evasion. To identify IC diagnostic biomarker candidates, we characterized the C. albicans hyphal secretome by gel-free proteomic analysis, and further assessed the antibody-reactivity patterns to this subproteome in serum pools from 12 patients with non-catheter-associated IC (ncIC), 11 patients with catheter-associated IC (cIC), and 11 non-IC patients. We identified 301 secreted hyphal proteins stratified to stem from the extracellular region, cell wall, cell surface, or intracellular compartments. ncIC and cIC patients had higher antibody levels to the hyphal secretome than non-IC patients. Seven secreted hyphal proteins were identified to be immunogenic (Bgl2, Eno1, Pgk1, Glx3, Sap5, Pra1 and Tdh3). Antibody-reactivity patterns to Bgl2, Eno1, Pgk1 and Glx3 discriminated IC patients from non-IC patients, while those to Sap5, Pra1 and Tdh3 differentiated between cIC and non-IC patients. These proteins may be useful for development of future IC diagnostic tests
Vultures from different trophic guilds show distinct oral pathogenic yeast signatures and co-occurrence networks
No full textVultures have evolved adaptive mechanisms to prevent infections associated with their scavenging lifestyle. However, food-borne exposure to antimicrobial pharmaceuticals can promote opportunistic infections with adverse outcomes. Here, we used multivariate and network analyses to increase understanding of the behavior of the yeast communities causing oral mycosis outbreaks recently reported in wild nestling cinereous (Aegypius monachus), griffon (Gyps fulvus) and Egyptian (Neophron percnopterus) vultures (CV, GV and EV, respectively) exposed to antibiotics from livestock farming. Common and unique yeast signatures (of Candida, Debaromyces, Diutina, Meyerozyma, Naganishia, Pichia, Rhodotorula, Trichosporon and Yarrowia species) associated with oral mycoses were identified in the three vulture species. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) highlighted that oral lesions from CV and GV shared similar yeast signatures (of major causative pathogens of opportunistic mycoses, such as Candida albicans, Candida parapsilosis and Candida tropicalis), while EV had a distinct yeast signature (of uncommon pathogenic species, such as Candida dubliniensis, Candida zeylanoides, Pichia fermentans and Rhodotorula spp.). Synergistic interactions between yeast species from distinct fungal phyla were found in lesions from CV and GV, but not in EV. These formed co-occurrence subnetworks with partially or fully connected topology. This study reveals that the composition, assembly and co-occurrence patterns of the yeast communities causing oral mycoses differ between vulture species with distinct feeding habits and scavenging lifestyles. Yeast species widely pathogenic to humans and animals, and yeast co-occurrence relationships, are distinctive hallmarks of oral mycoses in CV and GV. These vulture species are more exposed to antibiotics from intensively medicated livestock carcasses provided in supplementary feeding stations and show higher incidence of thrush-like oral lesions than EV. These findings may be useful for development of new initiatives or changes in the conservation of these avian scavengers affected by anthropogenic activities.This work was supported by the Ministry of Economy, Industry and Competitiveness from Spain (grant numbers CGL2009-12753-C02-01/BOS and CGL2010-15726)
Diario de Campo: Boletín Interno de los investigadores del área de Antropología. 94 (2007) octubre. Diario de Campo
No full text- De inexplicable por Elizabeth Romero Betancourt. - El niño Fidencio por Olimpia Farfán Morales. - El Lienzo de Moyotepec por Samuel Villela. - Cocina mexicana o historia gastronómica de la ciudad de México. Cuarenta años de un clásico por José Luis Juárez López. - La colección Fernando Iglesias Calderón por María Hernández Ramírez. - Internadas de Chalco. Los efectos del poder en el cuerpo por Josefina Ramírez Velásquez. - Chichén Itzá en la era de la mercantilización mediática por Jesús Antonio Machuca. - Y continúa la polémica. Unidad y diversidad en Mesoamérica por Saúl Millán. - Modus operandi... o de cómo los etnógrafos recurren a la historia y los historiadores a la etnografía por Marina Alonso Bolaños. - Trabajo agrícola y ritualidad: Notas para una reflexión sobre la unidad y la diversidad en Mesoamérica por Andrés Medina. - El canon prehispánico por Pedro Pitarch. - Suplemento 44. Religiosidad popular México-Cuba
Two-Stage Stochastic Scheduling of a Multiproduct Pipeline System using Similarity Index Decomposition
No full text[EN] Multiproduct pipelines are crucial for delivering substantial quantities of refined oil products from major supply centers to clients within a nearby geographical area. Despite the significant infrastructure investment, the associated transportation costs are markedly lower than those incurred with traditional delivery trucks. However, the scheduling of these systems presents a formidable challenge, requiring meticulous planning of pumping runs well in advance to meet the anticipated demands of clients. In this work, we enhance an existing literature model of a multiproduct pipeline system by introducing uncertainty in the customer demand. The problem is then addressed via a two-stage stochastic formulation. The typical drawback with stochastic formulations is the high computational burden required. To address this challenge, we adapt the so-called Similarity Index decomposition, resulting in a 28-fold improvement in CPU time while achieving equivalent solutions compared to solving the full-space problem.These results are funded by the Spanish MCIN/AEI/10.13039 /501100011033/, as part of the a-CIDiT (PID2021-
123654OB-C31) and LOCPU (PID2020-116585GB-I00) research projects. The first author has received financial
support from the 2020 call of pre-doctoral contracts of the University of Valladolid and Banco Santander.Montes, DA.;Pitarch, José Luis;De Prada, C. (2024). Two-Stage Stochastic Scheduling of a Multiproduct Pipeline System using Similarity Index Decomposition. IFAC-PapersOnLine. 58(14):43-48. https://doi.org/10.1016/j.ifacol.2024.08.311S4348581
The Similarity Index to Decompose Two-Stage Stochastic Scheduling Problems
No full text[EN] Two-stage stochastic scheduling problems often involve a large number of continuous and discrete variables, so fnding solutions in short time periods is challenging and computationally expensive. However, for online or closed-loop scheduling implementations, optimal or near-optimal solutions are required in real-time. We propose a decomposition method based on the so-called Similarity Index (SI). An iterative procedure is set up so that each sub-problem (corresponding to a scenario) is solved independently, aiming to optimize the original cost function while maximizing the similarity of the frst-stage variables among the scenarios solutions. The SI is incorporated into each subproblem cost function, multiplied by a penalty parameter that is updated in each iteration until reaching complete similarity in the frst-stage variables among all subproblems. The method is applied to schedule production and maintenance tasks in an evaporation network. The tests show that signifcant benefts are expected in terms of computational demands as the number of scenarios increases.These results are funded by the Spanish MICINN with FEDER funds, as part of the In CO4 In (PGC2018-099312B-C31) and LOCPU(PID2020-116585GB-I00) research projects. The first author has received financial support from the 2020 call of the pre-doctoral contracts of the University of Valladolid, co-financed by Banco Santander.Montes, D.; Pitarch, JL.; De Prada, C. (2022). The Similarity Index to Decompose Two-Stage Stochastic Scheduling Problems. IFAC-PapersOnLine. 55(7):821-826. https://doi.org/10.1016/j.ifacol.2022.07.546S82182655
El canon prehispánico. 94 (2007) octubre. Diario de Campo. Boletín interno de los investigadores del área de Antropología
No full textThe Structural Permissiveness of Triosephosphate Isomerase (TpiA) of Escherichia coli
No full textThe data that support the findings of this study are available from the corresponding Author upon reasonable request.Triosephosphate isomerase (TpiA) is widely regarded as an example of an optimally evolved enzyme due to its essential role in biological systems, its structural conservation, and its near-perfect kinetic parameters. In this study, we investigated the structural robustness of the archetypal TpiA variant from Escherichia coli using an in vitro 5-amino acid linker scanning method. The resulting library was introduced into a tpiA mutant strain for functional complementation. From this library, 16 TpiA variants that were phenotypically indistinguishable from the wild-type enzyme were selected for further analysis. Although all variants retained enzymatic activities within the wild-type range, several insertions were found in highly structured protein domains where the linker was expected to cause significant structural perturbations. Despite these potentially disruptive additions, the enzymes maintained their activity even when expressed in a dnaK mutant, suggesting that chaperones did not compensate for structural abnormalities in vivo. Additionally, when these mutant TpiA variants were produced using an in vitro transcription/translation system, they exhibited enzymatic activity comparable to, and in some cases exceeding, that of the non-mutated enzyme. AlphaFold2 exposed that insertions reconstructed the local architecture of the nearby amino acid sequences. The evolutionary implications of this remarkable structural resilience are discussed.Research in VdL Laboratory is funded by the NYMPHE (HORIZON-CL6-2021-UE 101060625) Contract of the European Union, the BIOSINT-CM (Y2020/TCS- 6555) Project of the Comunidad de Madrid - European Structural and Investment Funds (FSE, FECER) and Grant CEX2023-001386-S of the MICIU/AEI.Peer reviewe
El català al País Valencià: retracció i resistència
No full textThe use of Catalan has been restricted to rural areas in the País Valencià. This fact hinders it form becoming an adequate language for communication in our present industrial society. The author proposes the active linguistic fidelity to improve this situation
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