1,720,990 research outputs found
Nanodomain switching of hydrothermally synthesized epitaxial PbTiO3 thin films for data storage
No full textNanodomain switching dynamics of hydrothermally synthesized epitaxial PbTiO3 thin films for Tbit/inch2 data storage
No full textProduction of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution
No full textFermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pa-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing the ii, latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity; of 2.57 g/liter/h.We thank Mary Berlyn for kindly providing the CGSC strains.
This work was supported by the Korea-Australia International Cooperative
Project from the Korean Ministry of Science and Technology
(MOST) and by the Brain Korea 21 program
Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli
No full textA new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, fed-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 l. The final cell concentration, PHB concentration and PHB content of 194 g l(-1), 168 g l(-1) and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose l(-1) as a feeding solution, resulting in a high productivity of 4.6 g PHB l(-1) h(-1).We thankMary Berlyn for kindly providing the CGSC
strains. This work was supported by the Korea-
Australia International Cooperative Project from the
Ministry of Science and Technology (MOST)
Evaluation of retention loss phenomena in heteroepitaxial PbTiO3 thin films with single c-domain structure fabricated at low temperature for SPM-based data storage application
No full textAmine-impregnated silica monolith with a hierarchical pore structure: enhancement of CO(2) capture capacity
No full textA polyethylenimine-impregnated hierarchical silica monolith exhibited significantly higher CO2 capturing capacity than other silica-supported amine sorbents, and produced a reversible and durable sorption performance.This work was supported by the Carbon Dioxide Reduction & Sequestration R&D Center (CDRS), one of the 21st Century Frontier R&D Programs in Korea
Characterizations of nanosized PbTiO3/Nb-SrTiO3 with scanning nonlinear dielectric microscopy
No full textCHARACTERIZATION OF EPITAXIAL GROWTH OF PbTiO3 THIN FILMS ON (001) Nb-SrTiO3 FABRICATED BY HYDROTHERMAL SYNTHESIS METHOD
No full textProduction of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Metabolically Engineered Escherichia coli Strains
No full textRecombinant Escherichia coli strains harboring a plasmid containing a novel artificial polyhydroxyalkanoate (PHA) operon consisting of the Aeromonas PHA biosynthesis related genes and Ralstonia eutropha reductase gene were developed for the production of poly(3-hydroxybutyrate-co-hydroxyhexanoate) [P(3HB-co-3HHx)] from dodecanoic acid. By applying stepwise reduction of dissolved oxygen concentration (DOC) during the fermentation. the final dry cell weight, PHA concentration, and PHA content of 79 g/L, 21.5 g/L, and 27.2 wt %. respectively, were obtained in 40.8 h, which resulted in the PHA productivity of 0.53 (g/L)/h. The 3HHx fi action slowly increased during the fed-batch culture to reach a final value of 10.8 mol %. The 3HHx fraction in the copolymer could be increased by 3-fold when the Aeromonas hydrophila orf1 gene was coexpressed with the PHA biosynthesis genes.This work was supported by Procter
& Gamble Company
Characterization of Nanosized PbTiO3/Nb-SrTiO3 with Scanning Nonlinear Dielectric Microscopy
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