2 research outputs found
Genetic Diversity and Gene Flow among Three Chicken Populations in Nigeria Using Microsatellite Markers
No full textTo understand the level of genetic diversity among and within three improved locally adapted chicken populations in Nigeria, six microsatellite markers were used with 100 genomic DNA from Shika Brown (SB = 34), FUNAAB Alpha (FA = 33), and Noiler (NL = 33). The allelic and genotypic profiles of each representative from each population were determined through polymerase chain reaction amplification of the repeat region. Genetic diversity, genetic distance, level of inbreeding, polymorphism information content, and combined exclusion probabilities of markers (CPE/CPF) were analyzed using Microsoft Excel microsatellite toolkit, GenAlex, Microsatellite Analyser, FSTAT, and Poptree2. 416 alleles with 18.99% rare and 81.01% fixed alleles were observed across populations. The mean number of alleles was 23.111 ± 0.43, mean effective number of alleles was 16.975 ± 0.75, the expected heterozygosity was 0.940 ± 0.00, observed heterozygosity was 0.396 ± 0.02, mean PIC value was 0.937, and mean gene flow rate was 10.874 ± 0.817. The mean FIS was 0.579 ± 0.037 and the global FST was 0.023 ± 0.002. Nei’s genetic distance revealed that Shika Brown and the Noiler chicken populations were related (0.6985). The combined exclusion probability (CPE) across markers and populations was 0.999 (excluding a parent) and CPF was 1.000 (excluding both parents). The PIC/marker values across populations were greater than the minimum value of 0.5. High FIS and low FST value indicated a high inbreeding level within and low degree of genetic differentiation among the chicken populations. In conclusion, the microsatellite markers used are highly polymorphic and suitable for parentage analysis, control inbreeding, and could be used as baseline genetic information in conservation programs
Local government and leisure:: A study of two local authorities and their responses to the growth of leisure
No full textLocal authorities have a long history of providing recreation and leisure services, but since the 1970s leisure departments have expanded with a corresponding growth in the number and importance of professional officers to run them. Growing affluence and improvements in the quality of life have led to a recognition of the social importance of leisure provision, and more recently its economic importance has been established with the introduction of schemes aimed at rejuvenating local areas. This thesis investigates the internal workings of two neighbouring local authorities in the North East of England. It is a comparative study of the District Councils of Wear Valley and Chester-le-Street, focusing primarily on the importance of leisure services in the public sector. It draws attention to the importance of leisure to justify local government as elected bodies concerned with the overall well-being of their local communities. After considering historical, philosophical and sociological perspectives on leisure, and examining the role of public bodies in the provision of leisure, the thesis proceeds with a detailed analysis of leisure services in Wear Valley and Chester-le-Street in a period of dramatic change. An evaluation of the differences in each District and their responses leads to a consideration of the 'Enabling' role now expected of all local authorities. Although local government is an important element in the British political system, it has been subjected to a plethora of legislation since 1979, aimed at reducing public spending and questioning and controlling the role and purpose of local authorities. The comparison of the two Districts involves an investigation of their internal conduct and business. It finds that leisure services are an increasingly important reasons for maintaining elected local authorities
