607 research outputs found

    Genomic profiling for piroplasms in feeding ixodid ticks in the Eastern Cape, South Africa

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    Importation of tick-infected animals and the uncontrollable migration of birds and wild animals across borders can lead to geographical expansion and redistribution of ticks and pathogen vectors, thus leading to the emergence and re-emergence of tick-borne diseases in humans and animals. Comparatively, little is known about the occurrence of piroplasms in ixodid ticks in the Eastern Cape, South Africa, thus necessitating this study, which is aimed at detecting piroplasms (Theileria and Babesia) from feeding tick samples collected from cattle, sheep, and goats in selected sites in the Eastern Cape, South Africa. A total of 1200 feeding ixodid ticks collected from farm animals at selected homesteads were first subjected to molecular identification using mitochondrial 12S ribosomal RNA (rRNA) gene by PCR and were further tested for the presence of piroplasms through amplification of the 18S rRNA gene via nested-PCR followed by sequencing of the PCR products. The results indicated that 853 (71.1%) corresponded to the genus Rhipicephalus, 335 (27.9%) corresponded to genus Amblyomma, and 12 (1%) corresponded to genus Haemaphysalis. Amblyomma hebraeum and Rhipicephalus appendiculatus were the most common identified ticks from this study. The 18S rRNA nested-PCR revealed that 44 (3.7%) samples were confirmed positive for Theileria. A homology search for the generated sequences revealed a high percentage identity of 98-98.9% similarity to T. buffeli, T. orientalis, and T. sergenti in the GenBank. Based on the results obtained herein, we conclude that there is a big diversity of Theileria species; therefore, we suggest that this research should cover more geographical areas in order to reveal the true prevalence of this pathogen in the studied area because this will be a great step in the possible prevention of an outbreak that could have devastating effects on livestock production and human health in both the studied areas and South Africa at large.This research was funded by [South African Medical Research Council] grant number [OBI013]

    ESTIMATION OF VANADIUM IN SELECTED LEAVES FROM AKURE, NIGERIA BY ATOMIC ABSORPTION SPECTROPHOTOMETRY.

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    Estimation of the levels of vanadium in selected leaves from Akure, Nigeria was performed by atomic absorption spectrophotometry after dry ashing the samples. The highest mean level (9.92mgkg-1) of vanadium was recoded in Coloaxia esculentum, 1.24mgkg-1 was recorded for Tectona grandis, mangifera indica, Aealypha ciliata, Discorea rotundata and Vernomia amygdaline, while ND was recorded for Manihot palmate and Psidium guajava. The results showed that there were variations (CV = 54.17%) in the levels of vanadium in all the leaves analyzed and the levels were high compared to WHO commendations for some foods (mollusks, crustaceans, fish and mammals). Efforts to reduce eating high levels of it in leaves should be ensured by using different processing methods before consumption by animals and human

    Biological Immobilization of Lead from Lead Sulphide by Aspergillus niger and Serpula himantioides

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    The abilities of Aspergillus niger and Serpula himantioides to immobilize lead (Pb) from lead sulphide (PbS) were investigated. Upon growth on potato dextrose agar (PDA) amended with PbS which contained 10mM Pb, no visible solubilization activity was detected. A. niger produced biomass in greater quantity compared to S. himantiodes in both experimental and control media. With a ratio of 1.13, A. niger growth was stimulated on PbS-amended media while that of S. himantiodes, with a growth ratio of 0.46 was inhibited. Despite its smaller biomass, S. himantiodes accumulated over five times more Pb than A. niger with 85.7 ± 4.9 μmol of Pb per g of dry weight while A. niger contained 14.6 ± 1.3 μmol of Pb per g of its dry weight. Both fungi immobilized Pb through the formation of crystalline precipitates and energy dispersive x-ray analysis revealed the precipitates to be Pb containing. This transformation is of importance in the weathering of metalbearing rocks as mineral neogenesis

    Biological Immobilization of Lead from Lead Sulphide by Aspergillus niger and Serpula himantioides

    No full text
    The abilities of Aspergillus niger and Serpula himantioides to immobilize lead (Pb) from lead sulphide (PbS) were investigated. Upon growth on potato dextrose agar (PDA) amended with PbS which contained 10mM Pb, no visible solubilization activity was detected. A. niger produced biomass in greater quantity compared to S. himantiodes in both experimental and control media. With a ratio of 1.13, A. niger growth was stimulated on PbS-amended media while that of S. himantiodes, with a growth ratio of 0.46 was inhibited. Despite its smaller biomass, S. himantiodes accumulated over five times more Pb than A. niger with 85.7 ± 4.9 μmol of Pb per g of dry weight while A. niger contained 14.6 ± 1.3 μmol of Pb per g of its dry weight. Both fungi immobilized Pb through the formation of crystalline precipitates and energy dispersive x-ray analysis revealed the precipitates to be Pb containing. This transformation is of importance in the weathering of metalbearing rocks as mineral neogenesis

    Dr. Steele remembered as great teacher, friend and author

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    Article in the Johnson C. Smith Newsletter regarding the death of A.O. Steele

    Author Response.

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    Dysplastic Papilliferous Basaloid Ameloblastoma: Report of a Case

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    Papilliferous ameloblastoma is an uncommon histologic variant of ameloblastoma. About five cases have been reported in the English literature till date. All five showed squamous differentiation with keratin formation and were diagnosed as papilliferous keratoameloblastoma. The present lesionshows basaloid differentiation and mild dysplasia in addition to papilliferous projections, thus termed dysplastic papilliferous basaloid  ameloblastoma.  Ameloblastoma, the most common odostoma has not ceased to intrigue pathologists with its diverse histomorphological patterns. Therefore, we present this uncommon lesion in a 50 year old man with right mandibular swelling. Key words: Papilliferous, Basaloid, Ameloblastoma, Dysplastic, Nonhealing socket

    PROBLEMS OF DETERMINING THE BALANCE OF RIGHTS BETWEEN THE AUTHOR AND THE SOCIETYABOUT THE USE OF INTELLECTUAL PROPERTY IN THE HISTORY OF LEGAL STUDIES

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    The article is dedicated to the problem of defining the boundaries of fair use of the composition. By the end of the 18th century compositions became unique and original. Authorship wasn't evaluated from the standpoint of traditionalism. It was connected with genius of the author, originality of creativity. Philosophers, for example I. Kant, J. G. Fichte, G. W. F. Hegel, started considering the concepts of author, ideas, and forms of the composition. The article explains what the idea and form is, comparison of these concepts, and why forms of the composition should be protected by law while the idea can be spread easily. Also the article explains the factors and legal grounds of non-classical concept's appearance due to the increasing complexity of public relations

    Betty [Zoo] (Mrs. A.O.) to Mr. Silver, 10 April 1963

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    Personal correspondenc

    Ammonium oxidation at the oxic/anoxic interface

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    Applied Science
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