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Evaluation of bambara groundnut (Vigna subterranea (L.) Verdc) accessions for drought tolerance and yield performance using agro- morphological and physiological traits.
Masters Degree. University of KwaZulu-Natal, Pietermaritzburg.Abstract available in PDF
Agronomic Performance, Nutritional Phenotyping and Trait Associations of Okra (Abelmoschus esculentus) Genotypes in South Africa
Preliminary study on variability and heritability estimates of micronutrient composition in the immature fruits of okra (<i>Abelmoschus esculentus</i>) genotypes in South Africa
Preliminary study on variability and heritability estimates of micronutrient composition in the immature fruits of okra (Abelmoschus esculentus) genotypes in South Africa
Okra is one of the important fruit vegetable crop but is an under-utilized and neglected plant species in South Africa. The immature fruits of okra is a good source of protein, minerals and vitamins. Despite its contribution to food, nutritional and health security, this crop is rarely cultivated and researched in South Africa. The study was carried out to assess the genetic variability and heritability estimates using micronutrient content such as iron, zinc and manganese in the immature green fruits of okra plant for future use in the okra breeding programme to increase nutritional value. The experiment was conducted at the Roodeplaat research farm of the Agricultural Research Council in a randomized complete block design replicated three times. The analysis of variance showed highly significant differences for all of three micronutrients recorded. This indicated that there were high level of genetic differences among the genotypes studied. Phenotypic and genetic coefficient of variations, broad-sense heritability (repeatability) and genetic advance were also estimated for iron, zinc and manganese, which could be exploited in selecting suitable and potential okra parents when breeding for high micronutrient contents. The phenotypic coefficient of variation ranged from 23.04% in Zn to 75.48% in Fe. Furthermore, the highest values for the genetic coefficient of variation was found in Fe. High heritability estimates were observed in all mineral elements Fe (99.86%), Mn (99.72%) and Zn (96.32%) evaluated indicating potential genetic gain as percentage of mean for Fe (155.38), Mn (69.20) and Zn (45.79), respectively when breeding for these micronutrient traits. The present study revealed the genetic potential of the genotypes studied and their importance for use in the quality-breeding programme aimed towards addressing malnutrition in South Africa
Efforts in breeding cowpea for aphid resistance: a review
Cowpea is an important food and nutritional security grain legume crop in the semi-arid regions of sub-Saharan Africa. Its production is limited by biotic factors including pre-harvest insect pests such as aphids. Aphids cause yield reductions, promotes the growth of sooty moulds, honeydew and as a result reduce photosynthetic processes and rates. Various methods have been deployed to try to control the damage of aphids in cowpea plants. The methods include the use of chemical insecticides, cultural, mechanical, biological controls and integrated pest management. However, these methods have not been very effective as smallholder farmers growing cowpea in marginal areas cannot afford them. Hence, host plant resistance/genetic control is the best and effective method for controlling aphids in cowpea plantations. Breeding for aphid resistance is one of the effective methods that can sustain the production and productivity of the cowpea for longer periods. Furthermore, assessing the presence of genetic diversity can also help in sourcing genes of novelty for addressing this important issue to increase the production and productivity of the crop. Recently, scientists have used various strategies to try to solve the challenge of aphid damage on cowpea. The objective of this review was to document the research progresses on aphid resistance breeding of cowpea to facilitate the breeding and conservation of cowpea germplasm. The review serves as a baseline information to guide future cowpea breeding for resistance to aphids
Analyses of phytochemicals and nutritional compositions in Bambara groundnut (Vigna subterranea [L.] Verdc)
Bambara groundnut (Vigna subterranea (L.) Verdc) grains have variable phytochemical compositions and minerals vital for the food and feed industry. There is a need to exploit the genetic diversity of Bambara groundnut for breeding new varieties with enhanced seed yield, nutritional content and quality to develop various products. The objective of this study was to determine the compositions of phytochemicals and mineral elements present in Bambara groundnut genetic pool to identify superior and contrasting genotypes to guide product development and breeding. Seventy-five genetically diverse Bambara groundnut genotypes were field evaluated across four locations (Brits, Loskop, Mafikeng and Polokwane) using a 15 × 5 alpha lattice design with three replications during the 2020–2021 cropping season. Genotypes were profiled for fat, phenolic and flavonoids contents at the Agricultural Research Council (ARC) analytical laboratory in South Africa. Further, the genotypes were assessed for the contents of the following minerals: calcium (Ca), iron (Fe), potassium (K), phosphorus (P), zinc (Zn) and nitrogen (N). The nutritional content of the test genotypes varied significantly (P 6.00 %). ARC Bamb-40 and ARC Bamb-59 recorded a higher mean Fe content of 16.00 mg.100 g−1. ARC Bamb-2 was the top-performing genotype with high fat content (6 %), Ca (211.93 mg.100 g−1), and Zn (7.17 mg/100 g). Ca,K, and N contents displayed strong correlations (r > 0.60, P < 0.05). Phosphorus and Zn contents exhibited moderate correlations with Ca. Overall, the study selected genotypes ARC Bamb-73, ARC Bamb-19, ARC Bamb-9 and ARC Bamb-2 with high compositions of essential nutrients for product development or breeding. The selected genetic resources are valuable for trait integration and developing new breeding populations with enhanced nutrient compositions and agronomic and market-preferred traits
Bambara Groundnut (Vigna subterranea [L.] Verdc.) Production, Utilisation and Genetic Improvement in Sub-Saharan Africa
Bambara groundnut (Vigna subterranea [L.] Verdc.) is a nutritionally rich grain legume crop indigenous to Africa. It is tolerant to drought stress and has become adapted to grow under low input and marginal agricultural production systems in Africa and Asia. Bambara groundnut is an orphan crop, and represents a neglected and under researched plant genetic resource. Modern crop management, production technologies, and value chains are yet to be developed in Africa to achieve the potential economic gains from Bambara groundnut production and marketing. In sub-Saharan Africa (SSA) the production and productivity of Bambara groundnut is low and stagnant because of diverse abiotic and biotic stresses and socio-economic constraints. Improved crop management and post handling technologies, modern varieties with high yield and nutritional quality, value addition, and market access are among the key considerations in current and future Bambara groundnut research and development programs. This paper presents progress on Bambara groundnut production, utilization, and genetic improvement in SSA. It presents the key production constraints, genetic resources and analysis, breeding methods and genetic gains on yield, and nutritional quality and outlook. The information presented will guide the sustainable production and effective breeding of the crop in order to pursue food and nutrition security, and improve livelihoods through Bambara groundnut enterprises
Drought Tolerance Assessment of Okra (Abelmoschus esculentus [L.] Moench) Accessions Based on Leaf Gas Exchange and Chlorophyll Fluorescence
Physiological and complementary phenotypic traits are essential in the selection of drought-adapted crop genotypes. Understanding the physiological response of diverse okra genotypes under drought stress conditions is critical to the selection of drought-tolerant accessions for production or breeding. The objective of this study was to assess the levels of drought tolerance in preliminarily selected okra accessions based on leaf gas exchange and chlorophyll fluorescence to determine best-performing genotypes for drought-tolerance breeding. Twenty-six genetically diverse okra accessions were screened under non-stressed (NS) and drought-stressed (DS) conditions under a controlled glasshouse environment using a 13 × 2 alpha lattice design in three replicates, in two growing seasons. Data were subjected to statistical analyses using various procedures. A significant genotype × water condition interaction effect was recorded for transpiration rate (T), net CO2 assimilation (A), intrinsic water use efficiency (WUEi), instantaneous water use efficiency (WUEins), minimum fluorescence (Fo′), maximum fluorescence (Fm′), maximum quantum efficiency of photosystem II photochemistry (Fv′/Fm′), the effective quantum efficiency of PSII photochemistry (ɸPSII), photochemical quenching (qP), nonphotochemical quenching (qN) and relative measure of electron transport to oxygen molecules (ETR/A). The results suggested variable drought tolerance of the studied okra accessions for selection. Seven principal components (PCs) contributing to 82% of the total variation for assessed physiological traits were identified under DS conditions. Leaf gas exchange parameters, T, A and WUEi, and chlorophyll fluorescence parameters such as the ɸPSII, Fv′/Fm′, qP, qN, ETR and ETR/A had high loading scores and correlated with WUEi, the ɸPSII, qP and ETR under DS conditions. The study found that optimal gas exchange and photoprotection enhance drought adaptation in the assessed okra genotypes and tested water regimes. Using the physiological variables, the study identified drought-tolerant accessions, namely LS05, LS06, LS07 and LS08 based on high A, T, Fm′, Fv′/Fm′ and ETR, and LS10, LS11, LS18 and LS23 based on high AES, Ci, Ci/Ca, WUEi, WUEins, ɸPSII and AES. The selected genotypes are high-yielding (≥5 g/plant) under drought stress conditions and will complement phenotypic data and guide breeding for water-limited agro-ecologies
Phenotypic Diversity and Characterization of the Southern African Bambara Groundnut Germplasm Collection for Grain Yield and Yield Components
Bambara groundnut is a highly nutritious underutilized legume crop, which can be cultivated in unfavorable environments, has the potential to address food security and can significantly contribute to climate-smart agriculture. The objectives of this study were to determine the phenotypic diversity and characterize a southern African bambara groundnut germplasm collection for grain yield and yield components, to identify superior bambara accessions and to determine the correlation between measured traits. The 100 bambara accessions were planted in two different locations over two seasons. There was significant variability among the accessions, locations, seasons and their interactions for all traits. Low broad-sense heritability values (<0.5) were observed for almost all traits. Significant positive correlations between all measured traits were observed. High-yielding accessions; WS 42 (AS), MV 67-1, K 5, AS 9, SCORE 1 and SB 12-3 were identified. Bambara accessions 224 (RF-7684), 179 (AB 16-5C), 121 (Red Ex Zimbabwe), 62 (SB 8-3), 9 (SB 4-1), 181 (SB 8-1B), 89 (AS 20), 217 (RF-6221), 94 (K 5), 177 (S1 Sel2) and 74 (AS 5) were associated with a high grain yield, plant spread and plant height. These accessions could be used as potential parents in a breeding program for the development of high-yielding varieties
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