89 research outputs found
Pleuropulmonary blastoma in a 3-year-old boy:A case study
No full textPleuropulmonary blastoma (PPB) is a very aggressive and rare type of cancer that occurs mainly in children; however, it may also affect adults. PPB originates from the lung or the pleural cavity, but its cause is still unknown. PPB has four different subtypes based on whether it involves cysts and/or cancerous nodules, as well as considering the age at diagnosis and prognosis. Medical imaging plays an important role in the diagnosis of PPB to quantify the extent of metastasis of the tumour, such as in type II and type III, and to titrate the treatment strategy accordingly. In this paper, the case of a deceased 3-year-old boy, who was diagnosed with type II and III PPB, is presented, including an analysis on related medical images involving both ionising (chest X-ray and computed tomography) and non-ionising techniques (ultrasound), as well as the treatments that the child underwent. The patient received surgical resection of the tumour, followed by chemotherapy; however, local tumour relapse occurred, a second resection was not possible, and the relapsed tumour did not respond to chemotherapy leading to the death of the child.</p
Investigation on anti-proliferation properties of porcupine bezoar (hystrix brachyuran) extracts exposed on hela cells lines combined with electroporation technique
No full textElectroporation (EP) is a technique whereby the biophysical changes on the cells that induced external high-intensity electrical field pulses in order to enhance applications in the medical field. It is a molecular biology technique in order to create pores through a cell wall membrane, boost the permeability of the cell membrane, and support chemicals, drugs or DNA to be imported into Hela cells. While by combining electroporation (EP) technique with porcupine bezoar (PB) extract might reduce the proliferation of HeLa cells because this compound extract has the ability of anti-proliferation and also anti-angiogenesis properties for controlling cancer cell growth. This research concentrate on reviewing and analyses the basic concepts and methods of combining electroporation and porcupine bezoar (PB) extract as applied in cancer treatment application. The combination of this technique might be a new alternative for anti-cancer treatment. The combination of this technique might be a new way for anti-c ncer treatment
The Improvement of Polymerization Characteristics for Reflective Dye-Doped Polymer Dispersed Liquid Crystal (PDLC) Display
No full textThe addition of dichroic dye to liquid crystal (LC) display makes an optical shutter easily without polarizer. However, the dichroic dye induces one significant problem an inadequate contrast. The general reason for this is the less available UV light for polymerization due to absorption of UV light by trapped dye molecules in polymer matrix. This problem results into accumulation of residual pre-polymer molecules and induces slow polymerization that produces larger droplet size for LCs. This in turns produces insignificant electro-optical properties for dye-PDLC display. Our strategy proposed a new way to overcome this difficulty by adjusting the some factors, e.g., pre-polymer, initiator contents and curing time. © 2012 American Scientific Publishers.1
Investigation on Anti-Proliferation Properties of Porcupine Bezoar (Hystrix Brachyuran) Extracts Exposed on Hela Cells Lines Combined with Electroporation Technique
Full text linkElectroporation (EP) is a technique whereby the biophysical changes on the cells that induced external highintensity electrical field pulses in order to enhance applications in the medical field. It is a molecular biology technique in order to create pores through a cell wall membrane, boost the permeability of the cell membrane, and support chemicals, drugs or DNA to be imported into Hela cells. While by combining electroporation (EP) technique with porcupine bezoar (PB) extract might reduce the proliferation of HeLa cells because this compound extract has the ability of antiproliferation and also anti-angiogenesis properties for controlling cancer cell growth. This research concentrate on reviewing and analyses the basic concepts and methods of combining electroporation and porcupine bezoar (PB) extract as applied in cancer treatment application. The combination of this technique might be a new alternative for anti-cancer treatment. The combination of this technique might be a new way for anticancer treatment
Application of a novel high resolution widefield surface plasmon microscope in cell engineering, wound healing and development of new binding assays
No full textSurface Plasmon (SP) microscope systems are mostly built around
the prism based Krctschmann configuration. In thcsc systcms the generation of
Surface Plasmons (SPs) is achieved by p-polarized light striking a metallised
prism surfacc at a spccific angle and then monitoring thc intensity of the reOected
light. Thus in these systems. an image of the material can be obtained in terms of
an intensity map. in which the intcnsity of thc image is dcpendent on the way the
light couples into the SPs. The drawback of these systems is that lateral resolution
relies on the ability of plasmons to propagate along the metallised layer. The
lateral resolution is thus limited to a few microns. Therefore, a new microscope
systcm was developed. i.e. thc Widcficld Surface Plasmon Resonance (WSPR)
microscope. that is not only capable of analysing molecular interactions at high
vertical resolutions. but also enables SP imaging at much higher lateral resolution
than prism based systcms. The functionality of thc novel (WSPR) microscope has
been investigated by imaging a scquence of binding events between
micropattcrncd cxtracellular matrix proteins and their specific antibodies both in
air and real-time. Using the WSPR systcm a changc in contrast was observed with
each protein binding cvcnts. Images produced via the WSPR system were
analyzcd and comparcd qualitatively and quantitatively. The preliminary results
acquired for these binding studics between antibody/antigens dcmonstrate that the
WSPR systcm capablc of resolving features down to 260nm although the
theoretically proven lateral resolution of the WSPR system is -500nm. Cell
surface interactions undcr two diffcrent culture conditions. i.e. HaCaTs cultured
on SPR substrate with Transforming Growth Factor ~3 (TGF~3) (50ng/lII/) and
without TGF~3 were also invcstigated. It was found that I-IaCaTs cultured in the
presence of TGF~3 showed enhanced division and motility along with decreased
cell attachmcnt as compared with cclls maintained in TGF~3 free media. It is
believed that cellular signalling by TGF~3 is very important for enhancing tissue
development in wound rcpair. It is confirmed that the WSPR microscope
described here can be used to study sequential monomolecular layer of
antibody/antigen interactions binding cvents and examination of cell surface
intcrfacial intcractions at latcral scales of less than one micron without the need
for traditional immunoOuorescent labelling. These rcsults have significant
implications in the developmcnt of ncw brecd fast binding assays system and in
enabling high resolution detailed examination of the cell surface couplings and
ccll signalling proccsses involvcd in cell attachmcnt and migration
Investigation of Anti Cancer Proliferation Properties of Luffa Acutangula on Ht29 Using Electroporation Method
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