15 research outputs found
Contribution a l'analyse de l'expression du genome racinaire de Vicia faba : etude des conditions de preparation d'une banque d'ADNc des cellules meristematiques
No full textSIGLECNRS T Bordereau / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc
Full Length Research Paper - Detection of mesocarp oleoyl-thioesterase gene of the South American oil palm Elaeis oleifera by reverse transcriptase polymerase chain reaction
No full textThe thioesterase enzyme functions in lipid synthesis by cleaving the
acyl-ACP bond and liberating the fatty acid. Thioesterases have been
isolated from a number of plant sources. The gene for this enzyme was
detected in Elaeis oleifera by reverse transcriptase
polymerase chain reaction (RT-PCR), cloned and sequenced and found to
have considerable sequence similarity with other previously cloned
thioesterases. Its highest homology is to the Brassica napus
oleoyl-ACP thioesterase, 72% at the nucleotide level over the coding
region examined, and 83% identity (90% positives) at the amino acid
level
Detection of mesocarp oleoyl-thioesterase gene of the South American oil palm Elaeis oleifera by reverse transcriptase polymerase chain reaction
No full textThe thioesterase enzyme functions in lipid synthesis by cleaving the acyl-ACP bond and liberating the fatty acid. Thioesterases have been isolated from a number of plant sources. The gene for this enzyme was detected in Elaeis oleifera by reverse transcriptase polymerase chain reaction (RT-PCR), cloned and sequenced and found to have considerable sequence similarity with other previously cloned thioesterases. Its highest homology is to the Brassica napus oleoyl-ACP thioesterase, 72% at the nucleotide level over the coding region examined, and 83% identity (90% positives) at the amino acid level.
Key Words: Elaeis oleifera, mesocarp, oleoyl-ACP thioesterase, RT-PCR.
African Journal of Biotechnology Vol.3(11) 2004: 595-59
Short communication - somatic embryogenesis in date palm (Phoenix dactylifera L.) from apical meristem tissues from 'zebia' and 'loko' landraces
No full textThe shoot apical meristem from young suckers were used as sources of
explants for initiation of culture using MS basal medium which
contained 2,4-D. This was incubated at 27°C in the dark.
Callogenesis was observed as early as the second subculture. Continuous
subculture of the callus in the establishment medium at about the third
subculture from calls production, resulted in somatic embryo formation.
The somatic embryos were then transferred to MS medium without hormones
under light where they matured after about two subcultures and
developed into shoots. The shoots produced roots when transferred to a
medium which contained NAA at 0.1 mg/L
Somatic embryogenesis in date palm (Phoenix dactylifera L.) from apical meristem tissues from ‘zebia\' and ‘loko\' landraces
No full textThe shoot apical meristem from young suckers were used as sources of explants for initiation of culture using MS basal medium which contained 2,4-D. This was incubated at 27oC in the dark. Callogenesis was observed as early as the second subculture. Continuous subculture of the callus in the establishment medium at about the third subculture from calls production, resulted in somatic embryo formation. The somatic embryos were then transferred to MS medium without hormones under light where they matured after about two subcultures and developed into shoots. The shoots produced roots when transferred to a medium which contained NAA at 0.1 mg/L.African Journal of Biotechnology Vol. 4 (3), pp. 244-246, 200
Short communication - somatic embryogenesis in date palm (Phoenix dactylifera L.) from apical meristem tissues from 'zebia' and 'loko' landraces
No full textThe shoot apical meristem from young suckers were used as sources of
explants for initiation of culture using MS basal medium which
contained 2,4-D. This was incubated at 27°C in the dark.
Callogenesis was observed as early as the second subculture. Continuous
subculture of the callus in the establishment medium at about the third
subculture from calls production, resulted in somatic embryo formation.
The somatic embryos were then transferred to MS medium without hormones
under light where they matured after about two subcultures and
developed into shoots. The shoots produced roots when transferred to a
medium which contained NAA at 0.1 mg/L
Isolation of a kernel oleoyl-ACP thioesterase gene from the oil palm Elaeis guineensis Jacq.
No full textThioesterases play a central role in determining chain lengths of fatty acids in oil storage tissues and have been isolated from a number of plant sources. While in some species enzymes that are specialized for the predominant fatty acids in the tissues examined have been found, in others, enzymes that are active over a broad range were observed. We have isolated a cDNA clone from the developing kernel of the oil palm Elaeis guineensis which encodes a thioesterase enzyme. Its highest homology was to the Brassica napus oleoyl-ACP thioesterase with which it had 72% homology at the nucleotide level, over the coding region examined, and 83% identity (90% positives) at the amino acid level
Isolation of a kernel oleoyl-ACP thioesterase gene from the oil palm Elaeis guineensis Jacq.
No full textThioesterases play a central role in determining chain lengths of fatty acids in oil storage tissues and have been isolated from a number of plant sources. While in some species enzymes that are specialized for the predominant fatty acids in the tissues examined have been found, in others, enzymes that are active over a broad range were observed. We have isolated a cDNA clone from the developing kernel of the oil palm Elaeis guineensis which encodes a thioesterase enzyme. Its highest homology was to the Brassica napus oleoyl-ACP thioesterase with which it had 72% homology at the nucleotide level, over the coding region examined, and 83% identity (90% positives) at the amino acid level.
Key Words: Elaeis guineensis, kernel, oleoyl-ACP thioesterase, cDNA.
African Jnl Biotechnology Vol.3(3) 2004: 199-20
The Use of ISSR Marker for Analyzing the Genetic Diversity of Nigerian Date Palms (Phoenix dactylifera L.)
No full text
