86,699 research outputs found
Expression and distribution of leptin and ghrelin in the digestive ap-paratus of DIO (diet-induced obesity) zebrafish
No full textExpression and distribution of leptin and ghrelin in the digestive ap-paratus of DIO (diet-induced obesity) zebrafish
Maruccio L, Russo F, Arcamone N, Mania M, Randazzo B, Ciriaco E
In this report we analyzed the expression and distribution of leptin and ghrelin in the digestive apparatus of DIO zebrafish. Leptin is anorexigenic peptide while ghrelin is orexigenic one. They act as antagonists. In this study in order to develop an obesity phenotype adult zebrafish were divided into two dietary groups. The con-trol group was fed with Artemia (5 mg cysts/fish/day) once per day. The overfeeding group was fed three times per day with Artemia (60 mg cysts/fish/day). For calorie restriction, the zebrafish was fed with Artemia (2.5 mg cysts/fish/day) for 2 weeks after being overfed for 8 weeks. At the end of this period, after 24h of fasting, the zebrafish digestive apparatus were sampled for immunohistochemistry, western blotting and qRT-PCR techniques. Leptin and ghrelin immunoreactivity were found in the enteric nervous system and neuroen-docrine cells in overfeeding and control zebrafish intestine. The number of immunopositive cells is greater in DIO zebrafish than in control ones. In both groups leptin immunoreactive nervous fibers were found around ducts and vessels of liver. The presence of proteins were confirmed by western blotting analysis. By qRT-PCR, leptin and ghrelin mRNA levels are higher in the overfeeding zebrafish intestine and in control zebrafish liver. The immunological detection of ghrelin and leptin in control zebrafish are in agreement with literature data. In DIO zebrafish the results are similar to those found for obese mammals. In conclusion, this study represent a starting point to investigate the mechanisms involved in the regulation of appetite and energy balance in DIO zebrafish
CHELATION OF COPPER(II) IONS BY DOXORUBICIN AND 4'-EPIDOXORUBICIN - AN ELECTRON-SPIN-RESONANCE STUDY
No full textIl padule di Suese (Livorno) sito di importanza comunitaria: materiali per un piano di gestione
No full textNon-Covalent Complexes between DNA-Binding Drugs and Double-stranded Deoxyoligonucleotides: A Study by Ionspray Mass Spectrometry
No full textBinding of Epstein-Barr virus nuclear antigen 1 to DNA: inhibition by distamycin and two novel distamycin analogues
No full textModulation of the interaction between cellular or viral transcription factors and target DNA sequences may represent a potential experimental strategy to control proliferation of neoplastic cells as well as virus DNA replication. Distamycin represents a likely candidate to mediate such modulation by pharmacological means. In order to obtain more detailed information on structure-activity relationships of these compounds, we have analysed the effects of distamycin and two distamycin analogues on the binding of a recombinant protein, the Epstein-Barr virus nuclear antigen 1 (EBNA-1) to its target sequence of Epstein-Barr virus, containing the 12 bp palindromic consensus TAGCATATGCTA. The sequence selectivity in the binding of distamycin to DNA was evaluated by footprinting experiments, while the effects of distamycins on DNA-protein interactions was analysed by means of electrophoretic mobility shift assay. The data presented in this paper suggest that distamycin and its analogues differentially inhibit the interaction between DNA-binding proteins and target DNA sequences
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
[Newspaper Clipping: Author Claims Evidence of Second JFK Assassin #1]
No full textNewspaper article titled "Author Claims Evidence of Second JFK Assassin." The article states that author Richard J. Whalen concluded "that there is circumstantial evidence to support the theory of a second assassin in the shooting of President John F. Kennedy.
Thrombin generation in children with acute lymphoblastic leukemia: effect of leukemia immunophenotypic subgroups
No full textElevated plasma concentrations of endogenous thrombin generation markers and thrombotic events have been reported in children with leukemia. The aim of this study was to evaluate the effects of cancer and its treatment on thrombin generation (TAT levels) in children with acute lymphoblastic leukemia (ALL). The authors evaluated 32 children (23 M, 9 F) aged between 1 and 15 years (mean 6) affected by ALL (immunophenotypic subgroups: 16 common, 7 T, and 9 pre-B type). In all patients TAT levels at onset and after 5-6 doses of L-asparaginase were evaluated. TAT levels were higher in patients both at onset (13.04 ± 10.90 ng/L) and after the 5-6 doses of L-asp (19.41 ± 11.05 ng/L) with respect to controls (4 ± 1 ng/L) (p < .001 and p < .001). TAT levels after 5-6 doses of L-asp were higher than those at onset (p < .001). Factorial ANOVA showed that at onset there was a significant effect of leukemia immunophenotypic subgroups upon TAT levels (p < .05) and no effect of inherited thrombotic risk factors. These results indicate that in children with ALL an important role is played by acquired thrombotic risk factors, among which the indirect cancer procoagulant activity has its importance
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