1,721,018 research outputs found
Cultivation of edible mycorrhizal mushrooms
No full textThe edible mycorrhizal mushrooms include some of the world's most expensive foods and have a global market measured in US$ billions. Despite this, few have been cultivated with any degree of success, and certainly not in volumes that are likely to reverse the catastrophic declines in production that have occurred over the past 100 years. The main obstacles to their cultivation are their need to be associated with a host plant to successfully grow and fruit, contamination with other ectomycorrhizal fungi both before and after the establishment of plantations, and a general lack of understanding of each mushroom's trophic relationships, and biotic, edaphic and climatic requirements
Caratterizzazione anatomo-morfologica delle micorrize di Tuber maculatum Vittad. su Ostrya carpinifolia Scop
No full textCaratterizzazione anatomo-morfologica delle micorrize di Tuber maculatum Vittad. su Ostrya carpinifolia Scop
No full textMOLECULAR CLONING OF THE SMALL GTPASE CDC42 AND RHO-GDI GENES INVOLVED IN THE ACTIN CYTOSKELETON DEVELOPMENT IN THE ECTOMYCORRHIZAL FUNGUS T. BORCHII VITTAD
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Morphological and molecular characterisation of Pulvinula constellatio ectomycorrhizae.
No full textThe ascomycete Pulvinula constellatio was found to form competing ectomycorrhizal relationships with plants that had been inoculated with Tuber spp. and other edible ectomycorrhizal fungi. In order to be able to distinguish P. constellatio mycorrhizae from those of the Tuber spp., we determined the morphological and bio-molecular characteristics of P. constellatio. The complete sequence of the ITS regions was determined, in order to select specific primers. The ITS region was also studied using restriction fragment length polymorphism analyses with several restriction enzymes that allowed an unambiguous identification of the species
Morphological and molecular characterisation of mycelia from different Tuber species in pure cultures.
No full text• Pure cultures of Tuber maculatum , Tuber melanosporum , Tuber aestivum , Tuber macrosporum , Tuber rufum and Tuber brumale were isolated and characterized by morphological and molecular methods.
• The Tuber mycelia were isolated from fruit bodies and molecular identification was performed using specific primers, restriction fragment length polymorphism and/or sequence analyses of the ITS region.
• The species grew between 1.1 mm wk −1 and 14 mm wk −1 on the selected medium. The mycelium of different Tuber species showed several common morphological features such as hyphal anastomoses, vesicle formation and hyphal aggregation. Differences were found in the frequency of these morphological features and in the hyphal pattern. The isolated mycelia also showed differences in the hyphal branch angle, septal distance, hyphal diameter and rate of growth of the hyphae.
• This result opens the possibility of using pure mycelial cultures of Tuber spp. for experimental purposes and for the commercial production of infected truffle plant
Identification of ectomycorrhizal fungi of the genus Tuber by species-specific ITS primers.
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Identificazione di marcatori genetici per la caratterizzazione di specie di Tuber mediante analisi RAPD
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