51 research outputs found
Imaging resin-cast osteocyte Lacuno-Canalicular system at bone-bioactive glass interface by scanning electron microscopy
No full textThe morphology of the osteocyte lacuno-canalicular system at the bone-biomaterial implantinterface has not been fully investigated. In this study, the resin-cast scanning electron microscopy technique was used, for the first time, to image the lacuno-canalicular network within neoformed bone around bioactive glass ~BG! particles implanted in rat tibia bone marrow. The most salient finding was that the osteocyte canaliculi pass through the calcium-phosphorus layer formed at the bone-BG interface and reach the silica-rich layer of the reacted BG.Fil: Gorustovich Alonso, Alejandro Adrian. Comision Nacional de Energia Atomica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
No full textThe aim of the present study was to evaluate the biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds using a chick embryo shell-less (ex ovo) culture system. Chick embryos were divided into two groups: control (C) and experimental (E). Scaffolds were placed on the chorioallantoic membrane (CAM) in embryos of group E at 10 days of total incubation. The 45S5 Bioglass®-derived glass-ceramic scaffolds proved to be biocompatible in terms of the absence of inflammatory response at the implant site (CAM). Moreover, no alterations in the other end-points assessed, i.e. survival, stage of embryonic development and body weight, were detected. However, body length was greater in group E embryos than in group C embryos (p ≤ 0.05). A marked reduction (93%) in Ca content in the scaffolds was evidenced by energy-dispersive X-ray analysis at 5 days post-implantation. Calcium release from the scaffold implanted on the CAM might have been responsible for the restoration of the bone-like phenotype in chick embryonic skeleton of group E as detected by Alcian blue-Alizarin red double staining, as well as by histological and microchemical analyses. Conversely, the control embryos exhibited a chondrogenic phenotype. © 2008 Acta Materialia Inc.Fil: Vargas, Gabriela Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Vera Mesones, Rosa. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto Geonorte; ArgentinaFil: Bretcanu, Oana. Imperial College London; Reino UnidoFil: Porto Lopez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Boccaccini, Aldo R.. Imperial College London; Reino UnidoFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentin
Derivados óseos en el tratamiento de defectos periodontales infraóseos: estado actual
No full textEl tratamiento de los defectos óseos periodontales es un tema de permanente preocupación respecto a las diferentes alternativas terapéuticas. La utilización de rellenos óseos se acentúa día a día, sin embargo aún no hay consenso acerca de cual biomaterial es el ideal para tal fin. La utilización de derivados óseos en el tratamiento de defectos periodontales infraóseos permite la resolución del defecto, ya sea mediante la acción física de relleno o por la promoción de los procesos biológicos de reparación y/o regeneración , lo que se traduce clínicamente por la disminución de la profundidad de sondaje y ganancia de inserción clínica, independientemente del mecanismo de acción desencadenado. los derivados óseos utilizados para tal fin son: tejido óseo vital como los autoinjertos intra o extrabucales, tejido óseo no vital como el hueso desmineralizado o mineralizado tanto de origen humano como bovino.Fil: Gorustovich, Alejandro. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Periodoncia. Buenos Aires, Argentina / Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica. Buenos Aires, Argentina
A histologic study of bone response to bioactive glass particles used before implant placement: A clinical report
No full textThis clinical report describes the microscopic analysis of the postextraction bone response to bioactive glass particles used prior to titanium implant placement, after a healing period of 6 months. The clinical and radiographic follow-up were performed over a 3-year period after implantation.Fil: Stvrtecky, Roberto. Universidad de Buenos Aires; ArgentinaFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guglielmotti, María Beatriz. Universidad de Buenos Aires; Argentin
Efecto de la anemia experimental sobre la osteogénesis post-exodoncia en ratas
No full textLos efectos de la anemia sobre diferentes variables fisiológicas han sido motivo de permanentes estudios. La revisión bibliográfica muestra la inexistencia de estudios cuantitativos en relación al proceso de cicatrización ósea en el alvéolo post-exodoncia. El objetivo del presente trabajo fue evaluar histológica e histomorfométricamente la osteogénesis en el alvéolo post-extracción de los molares inferiores de ratas.Fil: Giglio, Máximo Juan. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Patología y Clínica Bucodental I. Buenos Aires, Argentina.Fil: Gorustovich, Alejandro. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica. Buenos Aires, Argentina.Fil: Guglielmotti, María Beatriz. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica. Buenos Aires, Argentina
Effect of bioactive glasses on Angiogenesis: a review of in vitro and in vivo evidences
No full textThe incorporation of bioactive glass into bone tissue-engineered scaffolds can be widely beneficial based on emerging evidence in the literature about the angiogenic potential of this material, particularly 45S5 Bioglass . This article reviews the literature discussing in vitro studies which have demonstrated that increases in angiogenic indicators have been achieved through both direct and indirect contact of relevant cells with 45S5 Bioglass particles or with their dissolution products. A few available in vivo studies confirming the ability of bioactive glass, incorporated into scaffolds, to stimulate neovascularization are also discussed. Suggestions for further research are given, highlighting the need for specific investigations designed to assess the effect of particular ion dissolution products from bioactive glasses and their relative concentration on angiogenesis both in vitro and in vivo.Fil: Gorustovich Alonso, Alejandro Adrian. Comision Nacional de Energia Atomica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roether, Judith A.. Imperial College London; Reino UnidoFil: Boccaccini, Aldo R.. Imperial College London; Reino Unid
A novel methodology for imaging newly bone formation around bioceramic bone substitutes
No full textIn this study, Frost's bulk‐staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol‐soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material.Fil: Gorustovich Alonso, Alejandro Adrian. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sivak, Matias Gabriel. Universidad de Buenos Aires; ArgentinaFil: Guglielmotti, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentin
Influencia de componentes dietarios en el téjido óseo : sonrisas sanas
No full textFil: Gorustovich, Alejandro A. Universidad de Buenos Aires. Facultad de Odonotología; ArgentinaFil: Steimetz, Tammy. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Nielsen, Forrest H. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Guglielmotti, María Beatriz. Universidad de Buenos Aires.Facultad de Odontología; ArgentinaDada la importancia nutricional de ciertos elementos minerales, como por ejemplo
fósforo, sodio, potasio, magnesio, cobre, zinc, silicio y boro, sobre la formación y
mantenimiento de los tejidos cartilaginoso y óseo, investigadores de la UBA, juntamente
con el Departamento de Agricultura de los Estados Unidos de Norteamérica (USDA), han
estudiado el efecto de la deficiencia nutricional de boro sobre la reparación, modelado y
remodelado del tejido óseo
Effects of Nutritional Deficiency of Boron on the Bones of the Appendicular Skeleton of Mice
No full textScientific evidence has shown the nutritional importance of boron (B) in the remodeling and repair of cancellous bone tissue. However, the effects of the nutritional deficiency of B on the cortical bone tissue of the appendicular skeleton have not yet been described. Thus, a study was performed to histomorphometrically evaluate the density of osteocyte lacunae of cortical bone of mouse femora under conditions of nutritional deficiency of B and to analyze the effects of the deficiency on the biomechanical properties of mouse tibiae. Weaning, 21-day-old male Swiss mice were assigned to the following two groups: Controls (B+; n = 10) and Experimental (B-; n = 10). Control mice were fed a basal diet containing 3 mg B/Kg, whereas experimental mice were fed a B-deficient diet containing 0.07 mg B/Kg for 9 weeks. The histological and histomorphometric evaluations of the mice fed a B-deficient diet showed a decrease in the density of osteocyte lacunae in the femoral cortical bone tissue and the evaluation of biomechanical properties showed lower bone rigidity in the tibia.. . . .Fil: Gorustovich Alonso, Alejandro Adrian. Universidad Católica de Salta; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; ArgentinaFil: Nielsen, Forrest H.. No especifíca
Bioactive glasses as delivery systems for antimicrobial agents
No full textMost biomaterial-associated infections are caused by opportunistic pathogens and bacteria that are regularly found within the microflora of the implant site. In addition, a biomaterial implant or device remains at risk of infection by hematogenous spread of bacteria disseminated from infections elsewhere in the body or from infected peri-implant tissue in revision surgery. The resulting infections are frequently accompanied by patient morbidity and discomfort and can lead to surgical replacement of the implant after lengthy, unsuccessful attempts to mitigate infections with antibiotic treatments. Therefore, extensive study is aiming to find new infection-resistant antimicrobial biomaterials and coatings for implants and devices to effectively reduce the incidence of biomaterial-associated infections. An overview of the in vitro and in vivo antimicrobial efficacies of the numerous biomaterials currently available is beyond the scope of this review. Herein, we provide a comprehensive review of bioactive glasses as biomaterial delivery systems for antimicrobial agents.Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Salta. Facultad de Ingeniería E Informatica. Instituto D/estudios Interdiciplinarios En Ingeniería; ArgentinaFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Salta. Facultad de Ingeniería E Informatica. Instituto D/estudios Interdiciplinarios En Ingeniería; Argentin
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