20 research outputs found
Effect of Red Raisins (Vitis Vinifera L.) Intake on the Level of Some Hematological Tests in a Group of Female Volunteers
No full textEffect of red beetroot (Beta vulgaris L.) intake on the level of some hematological tests in a group of female volunteers
No full textGenotyping of Type A Human Respiratory Syncytial Virus Based on Direct F Gene Sequencing
No full textBackground and objectives: The human respiratory syncytial virus (hRSV) is among the important respiratory pathogens affecting children. Genotype-specific attachment (G) gene sequencing is usually used to determine the virus genotype. The reliability of the fusion (F) gene vs. G gene genotype-specific sequencing was screened. Materials and Methods: Archival RNA from Saudi children who tested positive for hRSV-A were used. Samples were subjected to a conventional one-step RT-PCR for both F and G genes and direct gene sequencing of the amplicons using the same primer sets. Phylogeny and mutational analysis of the obtained sequences were conducted. Results: The generic primer set succeeded to amplify target gene sequences. The phylogenetic tree based on partial F gene sequencing resulted in an efficient genotyping of hRSV-A strains equivalent to the partial G gene genotyping method. NA1, ON1, and GA5 genotypes were detected in the clinical samples. The latter was detected for the first time in Saudi Arabia. Different mutations in both conserved and escape-mutant domains were detected in both F and G. Conclusion: It was concluded that a partial F gene sequence can be used efficiently for hRSV-A genotyping
Biochemical and molecular profiling of induced high yielding M3 mutant lines of two Trigonella species: Insights into improved yield potential.
No full textTrigonella, commonly known as Fenugreek, is among the most promising medicinal herbs consumed worldwide due its protein rich dietary contributions. This study involved induced mutagenesis on two Trigonella species (Trigonella foenum-graecum var. PEB and Trigonella corniculata var. Pusa kasuri) using caffeine and sodium azide as mutagens, resulting in the identification of nine high-yielding mutant lines in the M3 generation. Molecular characterization using SCoT markers revealed a high polymorphism of 28.3% and 46.7% in PEB and Pusa kasuri, respectively, facilitating the investigation of genetic divergence among the control and mutant lines. Similarity correlation analysis indicated a high similarity between mutant A and mutant C (0.97) and between mutant J and mutant O (0.88), while the lowest similarity was observed between mutant B and mutant F (0.74) and between control and mutant L (0.58). Mutant F and Mutant J displayed the highest seed yield and its attributing traits, and seed protein content in PEB and Pusa kasuri, respectively. Physiological parameters, including chlorophyll content (Mutants A and N) and carotenoids (mutant A and J), exhibited improvements. Assessment of stomatal and seed characteristics using scanning electron microscopy may lead to improved physiological processes and distinction at the interspecific level, respectively. Methanolic extracts of the control and the mutant lines of both species were subjected to GC-MS analysis, revealing 24 major phytocompounds known for their pharmacological activities (antioxidant, anti-inflammatory, anticancer, etc.). Statistical methods such as Pearson correlation heatmap and pairwise scatter plot matrix provided insights into the correlations and linear associations among parameters for both PEB and Pusa kasuri. The strong correlation between iron content and seeds per pod in the mutant lines suggests a promising avenue for further research. Continued research and breeding efforts using these mutants can lead to significant advancements in agriculture and medicine, benefiting farmers, consumers, and industries alike
Additional file 1 of Complete chloroplast genome of the desert date (Balanites aegyptiaca (L.) Del. comparative analysis, and phylogenetic relationships among the members of Zygophyllaceae
No full textAdditional file 1
Assessment of genetic diversity in bread and durum wheat landraces based on biochemical and in silico analysis of glutenin and gliadin proteins
No full textThis study assessed the genetic diversity between durum wheat landraces (L1-L12) and bread wheat landraces (L1-L12) using a comparative biochemical (SDS-PAGE) and bioinformatics analysis of their major gluten proteins: LMW-GSs, HMW-GSs, and gliadin fractions. Biochemical analysis revealed significant polymorphism in both species, with approximately 24-26% of the assessed loci showing variability. Cluster analysis based on the overall protein profiles successfully grouped all genotypes into three distinct clusters. Specific focus on the HMW-GSs and omega-gliadins revealed molecular markers related to end-use quality. Significant allelic polymorphism was detected at the Glu-1 loci: the Glu-A1 subunit 1 was fixed in durum wheat but rare in bread wheat, while Glu-A1 subunit 2 showed the inverse distribution. Diverse alleles (7, 8, 17, 18) were found at Glu-B1. Furthermore, bread wheat generally had five omega-gliadin subunits, compared to only four in durum wheat. Crucially, hierarchical clustering using the combined HMW-GS and omega-gliadin data provided a robust molecular signature, cleanly separating all genotypes into two clusters (Durum and Bread). This demonstrates the effectiveness of these two protein groups in distinguishing the wheat types, reflecting quality-related genetic differences. The subsequent bioinformatics analysis of 3D structures showed that most fractions (LMW-GSs, gamma-gliadin, and alpha/beta-gliadin) are highly conserved, with only minor variations in the N-terminal regions. In contrast, HMW-GSs and omega-gliadin exhibited significant structural divergence in their central repetitive domains. HMW-GS variations in beta-turn and beta-spiral distribution directly correlated with dough viscoelastic properties. Notably, durum omega-gliadin was found to be longer and contained more rigid polyproline type II helices, highlighting key structural differences influencing wheat quality
Crossbreeding parameters for body weight data from a complete diallel mating scheme using three breeds of rabbit
No full text<jats:p>Abstract. The objective of this study was to evaluate heterosis, general combining ability (GCA), maternal ability (GM), and sex-linked effects (SL) for growth performance from weaning (at 4 weeks) up to marketing age (at 12 weeks) using a complete 3×3 diallel crossing experiment in three different breeds of rabbit: one native Egyptian (Baladi Red, RR) and two exotic (Bauscat, BB, and Californian, CC). Offspring (2617 rabbits) body weight data (BW; 4–12 weeks of age) were analyzed, mainly to evaluate the effects of mating groups (MGs), sex, year season, and parity. MGs were further analyzed for crossbreeding parameters. All crossbreds showed positive, highly significant values (P≤0.001). Heterosis (HI; overall or specific) for body weight at all evaluated ages was highly significant (P≤0.001). Purebred differences were affected significantly (P≤0.01 or P≤0.001). No significant effects were detected due to general combining ability, maternal abilities, or reciprocal effect (sex-linked). Results suggest that to some extent and because of the highly purebred significant differences (P≤0.01 or P≤0.001), those three breeds are expected to be too genetically far apart to make crosses showing significant heterosis. Since other crossbreeding effects are non-significant, heterosis is the only reliable effect that can produce the Egyptian broiler rabbits when using those three breeds. However, the insignificance of the reciprocal impact (sex-linked as a whole or, by definition, the additive effects of genes carried on the sex chromosomes) of the two acclimatized standard breeds (CC and BB) seemed to be of magnitude at least to the 10th week of age.
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A Novel SPOTTED LEAF1-1 (SPL11-1) Gene Confers Resistance to Rice Blast and Bacterial Leaf Blight Diseases in Rice (Oryza sativa L.)
No full textProgrammed cell death (PCD) plays critical roles in plant immunity but must be regulated to prevent excessive damage. In this study, a novel spotted leaf (spl11-1) mutant was identified from an ethyl methane sulfonate (EMS) population. The SPL11-1 gene was genetically mapped to chromosome 12 between the Indel12-37 and Indel12-39 molecular markers, which harbor a genomic region of 27 kb. Annotation of the SPL11-1 genomic region revealed the presence of two candidate genes. Through gene prediction and cDNA sequencing, it was confirmed that the target gene in the spl11-1 mutant is allelic to the rice SPOTTED LEAF (SPL11), hereafter referred to as spl11-1. Sequence analysis of SPL11 revealed a single bp deletion (T) between the spl11-1 mutant and the ‘Shuangkang77009’ wild type. Moreover, protein structure analysis showed that the structural differences between the SPL11-1 and SPL11 proteins might lead to a change in the function of the SPL11 protein. Compared to the ‘Shuangkang77009’ wild type, the spl11-1 mutant showed more disease resistance. The agronomical evaluation showed that the spl11-1 mutant showed more adverse traits. Through further mutagenesis treatment, we obtained the spl11-2 mutant allelic to spl11-1, which has excellent agronomic traits and more improvement and may have certain breeding prospects in future breeding for disease resistance
Phytochemical profiling and SCOT marker analysis reveal genetic diversity in Ziziphus mauritiana genotypes
No full textThis study evaluated genetic diversity among 15 genotypes Ziziphus mauritiana based on biochemical and molecular markers to identify superior germplasm for breeding and nutritional improvement. Results of biochemical profiling revealed substantial variation as TSS ranged from 9.45 to 14.52 °Brix (‘G14’ and ‘G13’) respectively, total sugars were ranged from 7.25 to 10.53 g/100 g (‘G14’ and ‘G3’) correspondingly, moreover, Vitamin C was ranged from 29.47 to 68.53 mg/100 g (‘G12’ and ‘G2’) individually. Furthermore, highest antioxidant activity was recorded in ‘G13’ (39.65%) and peak of total phenolics was recorded in G5 (252.35 mg GAE/100 g). Principal component analysis (PCA) results revealed that genotypes ‘G3’, ‘G5’, ‘G6’, ‘G10’, and ‘G13’ as superior in sweetness, antioxidants, and secondary metabolites. Molecular markers (SCoT marker) analysis developed 112 bands, 67 polymorphic, with SCoT-2 being most informative (78.26% polymorphism, Ne = 1.37, h = 0.22, I = 0.33), reflecting moderate genetic diversity among the Z. mauritiana genotypes. However, Population structure and PCoA analysis revealed that there are two major genetic clusters constructed, with some genotypes showing admixture, indicating shared ancestry or gene flow and may be due to diverse genetic make and crossability among genotypes. The correlation matrix among phytochemical attributes displayed a positive relation between TSS and sugars with antioxidant, however, titratable acidity showed a negative relation with quality traits. Concludingly, current investigation pointed out nutritionally and genetically superior genotypes, providing a valuable resource for breeding, conservation and functional food development which is a useful information for future breeding programs
Assessing molecular and agronomic diversity in flax genotypes based on SCoT marker profiling, fibre yield contributing characters, and seed yield-related traits
No full textExploring genetic diversity is crucial for effective breeding strategies to enhance crop performance under diverse environmental conditions and accelerate selection with improved yield and adaptability to climate change. This study aimed to explore the genetic diversity of eleven flax genotypes (Linum usitatissimum L.) using Start Codon Targeted (SCoT) markers and evaluate their performance in fibre yield contributing characters and seed yield-related traits in two field trials during 2021-2022 and 2022-2023 seasons. SCoT markers revealed varying degrees of polymorphism, with primers SCoT-1, SCoT-24, and SCoT-27 displaying 100% polymorphism, indicating high genetic diversity among the genotypes. Genetic similarity analysis categorized the genotypes into four diverse groups. The agronomic performance indicated that ‘S.620’, ‘Sakha 3’, and ‘S.651’ excelled in straw yield and associated traits such as technical length, plant height, fibre percentage, total fibre yield, fibre length, and straw yield, while ‘Sakha 5’ and ‘S.945’ showed the lowest. On the other hand, ‘Sakha 5’ followed by ‘S.933/15/2’ and ‘Sakha 6' were the top performers for seed yield and related characteristics, including fruiting zone length, number of capsules per plant, seed index, seed yield, oil percentage, and seed and oil yields, while ‘IV2’ exhibited the lowest values. Hierarchical clustering divided the genotypes into distinct clusters based on straw and seed performance. Moreover, the principal component analysis highlighted a clear separation between genotypes excelling in straw and seed traits, with strong associations among related characters. These findings provide valuable insights for breeding programs to improve flax fibre and seed production
