1,721,121 research outputs found
Direct and cell-mediated effects of interferon-alpha and -gamma on cells chronically infected with HTLV-III.
No full textANTICANDIDAL ACTIVITY AND INTERLEUKIN-1-BETA AND INTERLEUKIN-6 PRODUCTION BY POLYMORPHONUCLEAR LEUKOCYTES ARE PRESERVED IN SUBJECTS WITH AIDS
Full text linkPolymorphonuclear granulocytes (PMN; or neutrophils) from uninfected or human immunodeficiency virus-infected subjects were tested for their ability to inhibit growth of Candida albicans and produce interleukin-1 beta (IL-1 beta) and IL-6 in vitro. It was seen that PMN from AIDS (Centers for Disease Control stage IV) patients expressed equal if not greater anticandidal activity compared with the activity expressed by neutrophils from all other subjects examined. On exposure to granulocyte macrophage-colony-stimulating factor or to a mannoprotein constituent (MP-F2) from C. albicans itself, PMN from AIDS patients showed enhanced antifungal activity and production of remarkable quantities of IL-1 beta and IL-6. These findings suggest that the functional abilities of PMN to inhibit Candida growth and secrete relevant proinflammatory and immunomodulatory cytokines are intrinsically preserved in AIDS patient
Mouse rosette forming cells and surface immunoglobulins in human lymphoid cells
No full textPeripheral blood lymphocytes from normal subjects were studied for mouse rosette-forming cells (MRFC) and their relationship to surface immunoglobulins (SIg). The majority of MRFC expressed both SIgM and SIgD, although some dissociation between population showing SIgM+/MRFC- and SIgM-/MRFC+ could be seen. A similar pattern of association was found in human derived cultured cells of B lineage, but no simple correlation and the number of SIgM+ cells could be established. Increased percentages of MRFC were detected in two foetal livers but greater dissociation of MRFC and SIgM was shown. This heterogeneity of MRFC may be explained by a selective expression of this surface marker in the early stages of B lymphocyte differentiation
130: Rosette formation with mouse erythrocytes by lymphocytes from normal donors and patients with various diseases.
No full textAnalysis of the cytolytic activity mediated by natural killer cells from acquired immunodeficiency syndrome patients in response to phytohemagglutinin or anti-CD16 monoclonal antibody.
No full textThe aim of this study was to assess the cytolytic potential of natural killer (NK) cells from human immunodeficiency virus type 1 (HIV-1)-infected patients, at different stages of the disease. Twenty HIV-1 seronegative donors as well as sixty HIV-1 seropositive patients were studied. Phytohemagglutinin and/or the anti-CD16 monoclonal antibody Kd1 were used to redirect the peripheral blood lymphocyte lysis of these patients to the 51Cr-labeled Fc gamma receptor-positive P815 murine mastocytoma target cell line. In parallel, NK cytotoxicity to tumor targets was investigated. Seronegative as well as HIV-1 Center for Disease Control (CDC) stage II patients showed maintained cytolytic activity. The cytolytic potential declined with disease progression, starting with CDC IVC2 patients, and was strongly diminished in acquired immunodeficiency syndrome stage patients. This defect was accompanied by decreased cytolytic activity to tumor targets and was not corrected by the in vitro addition of interleukin-2. The number of cells bearing a mature NK phenotype was normal in all the study groups. Our data suggest that the impaired NK cytotoxicity to tumor targets described during the progression of HIV-1 disease may be related to the progressive loss of function of surface receptors involved in NK cell triggering
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