1,720,972 research outputs found
Endometrial cytology in the bitch : nuclei measurement in different phases of the estrous cycle and in uterine disorders
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Characterization of a population of unique granular lymphocytes in a bitch deciduoma, using a panel of histo- and immunohistochemical markers
Full text linkThe ovaries and uterus were collected after ovariohysterectomy from a 16-month-old Labrador bitch in diestrus that never mated. Discrete swellings were found in the uterine horns, with the macroscopic appearance of normal early pregnancy. At histologic examination, the endometrium, devoid of any conceptus and chorion, showed a marked proliferation, on the basis of which a diagnosis of deciduoma was made. A remarkable population of stromal eosinophilic granular lymphocytes was present, especially in the axis of the endometrial folds. Periodic acid–Schiff and Dolichos biflorus–lectin histochemical reaction and a panel of 10 immunohistochemical markers were used to characterize eosinophilic granular cells. Our findings allowed us to compare these granular cells with the granulated decidual cells, whose presence was until now described only in primates, rodents, or a few other epitheliochorial species. On the basis of our results, the importance of eosinophilic granular cells in a decidualization process is hypothesized to occur also in the bitch
Effectiveness of electroacupuncture analgesia compared with opioid administration in a dog model: a pilot study
No full textBackground Although opioid analgesics are the usual drugs to treat post-surgical pain, acupuncture has also been demonstrated to relieve various pain syndromes. The present pilot study aims to investigate the efficacy of electroacupuncture compared with a conventional opioid compound, butorphanol, for postoperative pain treatment in dogs undergoing elective ovariohysterectomy. Methods Twelve dogs were randomly allocated into two groups. Dogs received either electroacupuncture stimulation (16 and 43 Hz) at Shen Shu, Chang Shu, He Gu, Tai Yuan, Zu San Li, Yang Ling Quan, and Bai Hui acupoints, while control dogs were treated with butorphanol. Cardiovascular and respiratory parameters were recorded for both groups during operation. Plasma β-endorphin concentrations were evaluated before surgery (baseline) and up to 24 h later. For each dog, pain was measured according to a dedicated subjective pain scoring system. Results Plasma β-endorphin levels in dogs receiving electroacupuncture increased significantly against baseline values after 1 and 3 h after surgery. Moreover, the end-tidal isoflurane concentration needed for second ovary traction was significantly lower in acupuncture-treated dogs than control animals. All animals having electroacupuncture experienced prolonged analgesia, over 24 h at least, while four out of six dogs treated with butorphanol needed post-surgical ketorolac and tramadol supplementation to their pain relief. Conclusions The results obtained from the present investigation showed some evidence for electroacupuncture as an alternative technique to provide postoperative analgesia in dogs
Rinvenimento di cellule endometriali in strisci colpocitologici nella cagna : interpretazione clinica
No full textIncubazione in liquido follicolare preovulatorio di spermatozoi di stallone freschi e crioconservati
No full textRecently, several studies were conducted to develop new artificial insemination techniques in horses based on reduced spermatozoa dose. Few data are available on intrafollicular insemination in mare. We analyzed morfological and functional changes of fresh and cryopreserved stallion spermatozoa incubated for six hours in preovulatory follicular fluid at 1:2 and 1:50 concentrations. Fresh sperm viability decreased from 78% to 57% at the dilution 1:2, whereas no changes were recorded for frozen spermatozoa (38% to 39%). At diluition 1:50 sperm viability showed a similar trend and there were no statistical differences with dilution 1:2. Either fresh and cryopreserved sperm showed a slight increase in acrosomal reactions during incubation at 1:2 dilution. At 1:50 dilution acrosome reactions in thawed sperm didn’t change significantly (25.86% to 18.42%), while for fresh sperm the variation from 22.58% to 51.28% risulted to be significant (P<0,05)
Seroprevalenece of brucellosis, chlamydiosis and Q fever in small ruminants in the periurban area of Niamey
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Risultati preliminari su attività sialiltransferasica ed acido sialico in siero e tessuti di cani sani e con neoplasie mammarie
No full textCorrezione dell'ernia diaframmatica congenita (CDH) in feti di pecora mediante intubazione selettiva del bronco principale di sinistra : studio preliminare
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