55 research outputs found
Tanzlingher's trilingual dictionary: the relative chronology of the Zadar (sine anno) and Zagreb (1679) manuscripts
No full textThe lexicographical production of Giovanni Tanzlingher Zanotti is at present known from four extant manuscripts, out of which the Zadar ms. lacks a date. Some formal characteristics put the Zadar ms. closer to the Zagreb ms. while distinguishing it from the Padua ms. The relation between the versions contained in the Zagreb and Zadar mss. is therefore analysed here. We will concentrate mainly on numerical and formal criteria, in order to minimize the risk of interpreting the contents of the two versions. Only when the proposed method will have produced a result it will be possible to interpret the differences and similarities between the two versions as a reflex of changing attitudes of the author toward his lexicographical endeavour
Ocular and skin temperature in ophthalmic postherpetic neuralgia
No full textNon-contact infra-red thermography was employed to measure the temperature of the eye and facial skin of a group of 12 patients with postherpetic neuralgia 5 months or more after the onset of ophthalmic shingles. The results indicate that the ocular surface on the affected side was significantly colder than its fellow (mean difference in temperature 1.08 ± 0.83oC). Skin temperature was not significantly different between the two sides. Several mechanisms may be involved in this process, including ocular ischaemia and sympathetic nervous system upregulation
Narrative dialectic of film of montage and of découpage - from silent era avant-garde to contemporary horror and thriller movies.
No full textEsta dissertação analisa os conceitos de cinema de montage (herança de procedimentos de montagem do cinema de avant-garde dos anos 1920) e cinema de découpage (herança do estabelecimento de cenas contínuas a partir do início do cinema falado), conforme desenvolvido pelo teórico americano David Bordwell. A dissertação elenca, ao longo da história do cinema, alguns dos principais cineastas que, dentro de um cinema narrativo de ampla difusão, equilibraram esses dois procedimentos. A trajetória proposta conduz ao cinema contemporâneo de terror e suspense, sustentando a idéia que estes são alguns dos gêneros mais propícios para o equilibrio de montage e découpage. Paralelamente, a dissertação analisa a trajetória dos filmes de seu autor, Philippe Barcinski. De seus primeiros curta-metragens (exercícios de montage) passando pelo seu primeiro longa-metragem (exercício de equilíbrio de cenas de montage e de découpage), a dissertação cria uma base teórica para seu próximo longametragem, em desenvolvimento, uma adaptação literária de um filme de suspense e terror.This work analyzes David Bordwell\'s concepts of montage (legacy of procedures of avant-garde cinema of the 1920s) and découpage (legacy of procedures of establishing continuity scenes from the beginning of the talkies). The text searches, throughout different moments of film history, some of the main director that, in narrative widely disseminated films, arranged those two procedures. The proposed path leads to the contemporary cinema of horror and suspense, supporting the idea that these genres naturally balances montage and découpage scenes. At the same time, the text analyzes the trajectory of its author, Philippe Barcinski, as a filmmaker. From his early short films (montage exercises) through his first feature film (which balances scenes of montage and découpage), the text proposes a theoretical basis for his next feature film in development, a literary adaptation for a thriller and horror movie
Application of electroabsorption in a semiconductor laser for electromagnetic field sensing
No full textThe application of electroabsorption in an AlGaAs quantum-well semiconductor laser device for optically based electromagnetic field sensing has been studied. A separate confinement heterostructure laser design containing a single quantum well and a steplike waveguiding index profile has demonstrated efficient modulation when operated as a light absorber rather than as an emitter.Observations of changes in the transmitted optical power through this device as a function of the applied forward and/or reverse dc bias have demonstrated nearly linear transfer functions for a given operating wavelength. Measurable changes in transmission were also observed for relatively small changes in applied voltage (100 V) allowing for the possibility of sensors with high sensitivity. Investigation of the high frequency operation of the device has shown a one-to-one correspondence in amplitude and phase between the applied RF voltage and the modulation of the transmitted optical power over a wide frequency range (1 GHz). Linear operation has been observed for a dynamic range exceeding 75 dB of the applied RF power at 20 MHz.Experiments that had coupled receiving antennas to a modulator have demonstrated the ability to measure an incident electric field of 34 V/m with a 300 MHz bandwidth and the corresponding time derivative of the magnetic field with a 30 MHz bandwidth. At 20 MHz, electric fields as small as 2 V/m have been measured. A 24 dB dynamic range in the measured electric field has been demonstrated and an expected range of 75 dB would imply the ability to measure fields larger than 10\sp4 V/m.The demonstrated performance characteristics of wide bandwidth operation and linear response with respect to the applied voltage make these types of semiconductor structures attractive for wide-band electromagnetic field sensing as well as for other low-power modulation applications.Made available in DSpace on 2011-05-07T14:11:31Z (GMT). No. of bitstreams: 2
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ASPAC: Amsterdam Slavic Parallel Aligned Corpus
No full textThis folder contains metadata on the Amsterdam Slavic Parallel Aligned corpus. This tool for linguistic research on Slavic language(s) is continuously expanded as texts and parallel translations are added. The data shared here describes its current state.
It includes data on the composition of the Corpus, its compilation and application in research projects.
The following files are shared:
01 General introduction and Purpose.pdf/A
02 Description of ASPAC Metadata 2023.pdf/A
03 ASPAC Metadata 2023.xlsx
Please note that it is currently not possible to include the contents of the corpus. Use of the corpus is strictly limited to invited guests. Please contact the author for further details.</p
Fonds Emile Prisse d'Avennes sur l'Egypte : Iconographie. Dessins, estampes, photographies (NAF 20434-20443). « Thèbes — Karnac, 1 »
No full textContient : Schroëder (Friedrich) d'après Jollois (Jean-Baptiste Prosper), Villiers du Terrage (Edouard de), Coraboeuf (Jean-Baptiste) et St Genis. Thèbes : Plan général de la portion de la vallée du Nil qui comprend les ruines / Jollois, Devilliers, Coraboeuf et St Genis delt ; Schroëder st ; Schroëder (Friedrich) et Blondeau d'après Jollois (Jean-Baptiste Prosper), Villiers du Terrage (Edouard de), Coraboeuf (Jean-Baptiste) et St Genis. Thèbes, Karnak : Plan topographique des ruines / Jollois, Devilliers, St Genis et Coraboeuf delt ; Schroëder st ; Louvet d'après LePère (Jean-Baptiste). Thèbes, Karnak : 1. 2. 3 Plan, coupe générale et élévation du palais, 4 Plan d'un petit temple, près de l'enceinte du palais / LePère Arch. Delt ; Louvet St ; Prisse d'Avennes (Emile). Palais de Karnac : Chapelle d'Amon construite en albâtre par Thotmes 3 : A. du plan général ; Prisse d'Avennes (Emile). Temple d'Amon dépendant du palais de Karnac : B du plan général ; Prisse d'Avennes (Emile). Karnak : Morceaux de plans ; Monecke (C.) d'après Erbkam (Georg Gustav). Theben. Memnonia : Grundrisse zum Vorbau de grossen Tempels von Medinet Habu (KK). MM : Temple am Südlichen Ende des Sees ; MM : Durchschnitt nach der Linie a.b. ; LL : Tempel am nördlichen Ende des Sees / Aufgen. u. gez. v. G. Erbkam ; Lith. v. C. Monecke ; Baltard. Karnak. Temple de Khons : Vue intérieure et plan du grand temple du Sud / Baltard Sct ; Plan : Avenue de Criosphinx ; Bury (Jean-Baptiste-Marie) ? d'après Prisse d'Avennes (Emile). Gournah. Palais de Ménephtah Ier, à Karnac. Egypte / E. Prisse del. ; Bury sculpt ; Thèbes. Karnac : Plan général des ruines / Levé et dessiné par Prisse d'Avennes ; Gravé chez Erhard Schièble rue St André des Arts 14. — Paris : Gide et Baudry, Editeurs (Paris : Imp. chez Kaeppelin, Quai Voltaire, 17) ; Thèbes. Karnac : Plan général des ruines / Levé et dessiné par Prisse d'Avennes ; Gravé chez Erhard Schièble rue St André des Arts 14. — Paris : Gide et Baudry, Editeurs (Paris : Imp. chez Kaeppelin, Quai Voltaire, 17) ; Prisse d'Avennes (Emile). Palais de Karnac : Plan de l'Akn-menou, temple de Thoutmosis III ; Bury (Jean-Baptiste-Marie) ? d'après Prisse d'Avennes (Emile). Temple de Khons à Karnac. Egypte / E. Prisse del. ; Bury sculpt ; Du Camp (Maxime). Temple de Dandour ; Prisse d'Avennes (Emile). Vue générale du palais de Karnak prise des piliers cariatides de l'est ; Jarrot (Edouard Athanase) ? Vue générale du palais de Karnak prise des piliers cariatides de l'est comme mon aquarelle ; Gournah. Temple de Menephtehum : Plan, coupe et élévation ; Du Camp (Maxime). Philae. Grand Temple d'Isis : Proscynéma ; Du Camp (Maxime). Kalabsha : Ptolémée Césarion ; Du Camp (Maxime). Karnak : Temple de Khons ; Du Camp (Maxime). Palais de Karnak : Sud-ouest ; Du Camp (Maxime). Karnak : Pilier devant le sanctuaire de granit ; Du Camp (Maxime). Medinet Habou : Pérystyle du palais de Rhamsès Méiamouni et ruines d'une église copte ; Du Camp (Maxime). Karnak : La cour des Bubastites ; Du Camp (Maxime). Karnak : Porte septentrionale de la salle hypostyleNumérisation effectuée à partir d'un document original.Appartient à l'ensemble documentaire : BbLevt
Cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay
No full textThe ability of tumor cells to invade is one of the hallmarks of the metastatic phenotype. To elucidate the mechanisms by which tumor cells acquire an invasive phenotype, in vitro assays have been developed that mimic the process of cancer cell invasion through basement membrane or in the stroma. We have extended the characterization of the circular invasion assay and found that it provides a simple and amenable system to study cell invasion in matrix in an environment that closely mimics 3D invasion. Furthermore, it allows detailed microscopic analysis of both live and fixed cells during the invasion process. We find that cells invade in a protease dependent manner in this assay and that they assemble focal adhesions and invadopodia that resemble structures visualized in 3D embedded cells. We propose that this is a useful assay for routine and medium throughput analysis of invasion of cancer cells in vitro and the study of cells migrating in a 3D environment
"Sometimes I feel that this is the Russia we had always dreamed of..." : transnationalism and capitalism ; migrants from the former Soviet Union and their experiences in Germany; paper for the conference 'Alltag der Globalisierung. Perspektiven einer transnationalen Anthropologie', January 16-18, 2003, Institute of Cultural Anthropology and European Ethnology, Johann Wolfgang Goethe University, Frankfurt am Main
No full textThe present article explores perceptions and cultural constructions of the terms capitalism or capitalistic West among ex-Soviet, highly qualified Jewish migrants from Russia and Ukraine after their emigration to Germany between 1990 and 1996. It seems that migration offers a unique opportunity to migrants to realise knowledge that is normally taken for granted, behaviour schemes and values, and to reflect on them. How do they acquire such presumed capitalist knowledge of the new society and new social world, how do they create it, and with what concrete contents do they connect the illusion about monolithic cultural, economic and political capital, the illusion which contributes to group formation and which serves as action orientation? As my research shows, immigrants try to disparage much of what appeared to them in the Soviet Union as normative, right and appropriate; now they often act by way of categories, which were defined in the previous context as "capitalist" and were interpreted as immoral. Without exact ideas or knowledge about behaviour codes, unspoken norms and silent values from the new society, many immigrants orient themselves towards the opposite of what was counted as morally proper in the origin society. Simultaneously they revive old system through the establishing and development of a Russian language enclave. Nevertheless this enclave is not located in a vacuum of "dusty" memories from the past, but build transnational cross-border space connected and corresponding to the processes of to-day's CIS and with the life of those relatives and friends who still live there, und with whom the emigrants share intensive social networks
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Developing Genetically Encoded Optical Tools for Imaging Brain Circuits and Pharmacology
No full textThe brain is an intricate organ that controls various functions, such as perception, behavior, mood, and cognition, through interactions between neurons, astrocytes with neurotransmitters (NT), and neuromodulators (NM). The recent advancements in fluorescence imaging and genetic engineering have allowed researchers to study neural activity, neurochemicals, and drug-specific receptor conformations at a cellular and subcellular level.Imaging neuronal spikes is crucial in the study of neural circuits and behavior. Neuronal spikes, or action potentials, are brief electrical signals traveling along nerve fibers and are the primary means neurons communicate. Researchers can directly visualize and quantify these electrical signals in living animals with high spatiotemporal resolution using genetically encoded calcium indicators (GECIs) and genetically encoded voltage indicators (GEVIs). This ability to image neuronal spikes in real-time in behaving animals offers new possibilities for dissecting neural circuits and understanding the mechanisms behind behavior across different species. In the past ten years, GECIs and GEVIs have revolutionized systems neuroscience by allowing for the mesoscopic recording of intracellular calcium as a proxy for electrical activity and directly reporting spiking patterns and subthreshold voltage activity.
Calcium and voltage imaging are valuable tools for monitoring neurons' calcium and electrical activity, but they have limitations when studying neuromodulation. Neuromodulation involves controlling neural activity through signaling molecules like hormones, NT, NM, and growth factors. This is a crucial aspect of brain function, enabling communication between neurons and coordination of brain regions. Neuromodulation fine-tunes neural circuits and influences brain functions like learning, memory, perception, and behavior. For instance, dopamine in the striatum controls reinforcement learning and habit formation, while serotonin affects mood, anxiety, and sleep. Neuromodulation also plays a crucial role in treating neurological and psychiatric disorders, with drugs altering NT levels or neuromodulatory patterns, such as antidepressants targeting the serotonin system and antipsychotics targeting the dopamine system. However, the release of NM may not be directly coupled to neuronal activation because NM is often released from non-synaptic locations, can have diffuse effects on multiple neurons, and can be released in response to non-neuronal signals.
Many groups have pioneered the effort and developed technologies for measuring the bulk release of NM, including micro-dialysis, opto-dialysis, amperometry, and mass spectrometry. However, the traditional methods either need direct measurements of specific NM or need to be higher in spatiotemporal resolution. Therefore, to better dissect the complex dynamics of neuromodulation, it is necessary to invent new technologies for monitoring NM release with sub-cellular and sub-second spatiotemporal resolution in real-time.
My graduate thesis focused on engineering genetically encoded neurochemical indicators (GENIs) and using optical imaging to study NM release. By genetically encoding the indicators, we can achieve specific cell-type targeting of the indicators and pinpoint the release pattern of specific NM in a highly specific manner, providing sub-second temporal- and sub-cellular spatial- resolution. This enables researchers to accurately measure the real-time release of NM, including their precise location and release dynamics in behaving animals. Overall, using GENIs and optical imaging to study NM release has greatly advanced our understanding of the complex processes involved in synaptic communication and has opened new avenues for exploring the mechanisms underlying neural and physiological diseases.
In the opening chapter, I delve into the current collection of GENIs, recent advances in NM dynamic studies, and the impact of drugs and environmental stimuli on neuromodulation via fluorescence imaging with GENIs developed by our lab and others. I present the methodology of engineering GENIs that allow real-time tracking of various neural activities and specific receptor conformations affected by drugs. I also use mathematical modeling to explore the engineering and optimization methods for these indicators. Further, the GENI engineering methods can be utilized for other neurochemicals we haven’t explored and provide a comprehensive toolkit for studying neural activity and drug effects in living organisms. The text of this chapter is modified from my first-author manuscript published in the Annual Review of Neuroscience in 2022.
Despite the essential biological functions that serotonin regulates, as aforementioned, imbalances in the serotonin system have been linked to several psychiatric and neurological disorders, such as depression, anxiety, and obsessive-compulsive disorder. Thus, understanding the underlying mechanisms of serotonin signaling is crucial for developing new treatments for these conditions. In chapters two and three of this dissertation, I expand into engineering two types of GENIs for the NM, serotonin.
To understand the pharmacological mechanisms of drugs on the serotonin system, in chapter two, I present the creation and validation of a GENI, psychLight, based on the 5-HT2A G-protein coupled receptor (GPCR) and circularly permuted green fluorescent protein. After extensive screening and optimization with molecular cloning and live-cell imaging techniques, psychLight was generated. It allows for detecting changes in serotonin signaling in behaving rodents under aversive stimuli. Additionally, I use psychLight to study the mechanism of action of designer drugs on receptors. By evaluating the biased receptor conformations upon ligand binding, I investigate how to eliminate the side-effect of psychedelic-based antidepressants, i.e., hallucination. I use genetic and viral approaches to develop a cell-based drug screening platform using psychLight (patented), resulting in the synthesis and discovery of novel compounds with both short-term and long-term antidepressant potential but without the hallucination side-effect. Finally, I demonstrate the use of psychLight and other genetic tools to show that psychedelics promote plasticity through intracellular 5-HT2A receptors, and serotonin may not be the natural ligand for those intracellular receptors. This highlights the importance of considering the cellular location of 5-HT2ARs in determining their signaling properties and suggests that intracellular 5-HT2ARs may be a valuable therapeutic target.
In summary, psychLight provides high-spatiotemporal resolution and real-time monitoring of endogenous serotonin in response to behavioral stimuli. The application of psychLight combined with other methods shed light on the mechanisms behind the therapeutic effects of psychedelics and the role of serotonin in promoting brain plasticity. Furthermore, the psychLight drug screening platform demonstrates the potential for conformational indicators in discovering novel treatments for neuropsychiatric and neurodegenerative diseases with fewer side effects. The text of this chapter is modified from my first-author manuscript published in Cell in 2021 and my contributions to the manuscript published in Science in 2023.
Chapter three presents an innovative method for creating a high-dynamic range serotonin indicator, iSeroSnFR, for better understanding serotonin’s role in the brain with a high signal-to-noise ratio (SNR). I use machine learning algorithms to modify an acetylcholine-binding protein, resulting in its ability to bind serotonin selectively. I validated the iSeroSnFR’s serotonin selectivity with screenings in mammalian cells and neuronal cultures. Together with others, we use iSeroSnFR to reveal serotonin dynamics during sleep-wakefulness cycles. The text of this chapter is modified from my contributions to the manuscript published in Cell in 2020.
In chapter four, I discuss the creation of GENIs to detect neuropeptides (NP), another class of NM. NP has been linked to various brain dysfunctions, including addiction, cognitive disorders, and stress. However, the study of NP signaling is minimal due to the nature of the degradation of peptides over time. Traditional methods that need to extract NP out of the brain for analysis cannot provide detailed enough information on NP signaling. Thus, a direct measurement at the site of release/reception is the key. Of the numerous NP discovered, opioids are clinically the most significant, as they are the primary target of effective pain-relieving treatments. However, these treatments also lead to issues of abuse and overdose. To create a treatment without such adverse side effects, a better understanding of opioid signaling and opioid receptor actions on drugs is necessary.
To fill this knowledge gap, I engineered a set of GENIs based on three opioid receptors to detect endogenous opioids with sub-second temporal precision at the releasing site in real-time. I thoroughly evaluated the binding kinetics of analgesics by comparing drug screening on the indicators and traditional radio-ligand binding assay on the receptors. By incorporating these indicators with optogenetics, a technology that allows using light to activate specific neuronal projections in the brain, I observed light-induced opioid release on opioid-releasing neurons in behaving animals. Further, I observed different opioid-releasing patterns in the sub-brain regions in rewarding and aversive behaviors with optical imaging. The opioid GENI toolkit is a valuable resource that facilitates new insights into opioid signaling and drug mechanisms that were previously inaccessible. The manuscript of this chapter is finalizing for publication.
Chapter five of the thesis focuses on the future outlook, discussion, and conclusion. I begin the chapter with preliminary data on the optimization of the next generation of gastrin-releasing peptide (GRP), a critical neuropeptide for regulating fear extinction. In this section, I present an ongoing project that aims to optimize the sensitivity from a previous generation GRP sensor, grpLight1.0. Given that we have published the grpLight1.0 in 2021 and used this sensor to reveal that bombesin-like peptide plays a crucial role in enhancing fear memory by recruiting disinhibitory cortical circuits. However, grpLight1.0 is not sensitive enough to detect functionally relevant GRP levels in vivo. The first section of the chapter provides results for the sensitivity optimization of grpLight1.0 with characterizations in vitro and preliminary data for monitoring GRP release in vivo during fear conditioning. This optimized sensor variant, grpLight2.0, will be followed by more detailed ex vivo and in vivo characterizations for further dissecting GRP’s role in the brain. In the later sections, I discuss the limitations of GENIs in the field of neuroscience and present a mathematical model for potential GENI optimization directions. Finally, I summarize my thesis projects and provide a future outlook.
Together, this thesis presents the methodology and applications of GENIs to study the dynamics of selected NM signaling and drug-receptor interaction in the brain. It provided valuable insights into the role of NM in shaping circuit function, both in healthy and diseased conditions. It highlights the need for better indicators of existing and other NM for a broader understanding of brain computation. Optimizing indicators for better SNR and expanding the toolkit for multiplex imaging with indicators on different spectrums is encouraged to perform multi-NM readouts simultaneously. Methods including machine learning and fluorescence-activating cell sorting are also suggested to enhance the indicator optimization process in the future. This thesis presents the significance of GENIs in neuroscience research and their possibilities in furthering our understanding of neural circuits in complex behaviors and pharmacology with precision
Abstract 259: A case series of vitreoretinal lymphoma in New York City with exposure to Chernobyl nuclear disaster decades earlier
No full textAbstract
Background: Vitreoretinal lymphoma (VRL) is an unusual presentation of primary central nervous system lymphoma and a subcategory of intraocular lymphoma (IOL). The etiology and epidemiology of this rare malignancy is unknown.
Methods: We diagnosed 10 subjects with VRL in our clinical practices in New York City (NYC) during 2010-2013. Upon systematic collection of demographic and exposure history, 6 patients reported residence in regions proximal to the Chernobyl nuclear disaster (4 Ukraine, 1 Poland, 1 Moldova). We studied clinical variables including presentation, diagnostic parameters, therapies and survival of this case series. To provide context, we examined population-based incidence data from the Surveillance, Epidemiology and End Results (SEER) program to determine age-adjusted incidence rates of IOL (there is no unique code for VRL) per 100,000 person-years in the United States (US) during 1992-2013. New York State (NYS) rates are not reported to SEER and were examined separately using NYS Cancer Registry (NYSCR). Incidence trends using 10-year intervals (1992-2002 and 2003-2013) were also analyzed.
Results: All six subjects (5 females) were diagnosed with diffuse large B-cell lymphoma with bilateral presentation. Diagnosis in all cases was made by immunohistochemical analysis on tumor specimen obtained by vitreous fine needle aspiration, vitreous biopsy or stereotactic brain biopsy. Median age at diagnosis was 76 years (range, 62-84 years). Median interval from Chernobyl accident (1986) to diagnosis was 26 years (range, 24-27 years). We identified no other common exposure or environmental risk factor. There were 68 cases of primary IOL during 1992-2013 in the 13 SEER areas. IOL rates per 100,000 person-years for both sexes were highest among Asian/Pacific Islanders (0.010 for males and 0.014 for females) followed by whites (0.009 for males and 0.008 for females) and (based on small numbers) by blacks. Analysis of incidence trends showed an increase in rates among Asian/Pacific Islander females from 0.005 in 1992-2002 to 0.018 in 2003-2013 and among males from 0.006 to 0.013. Rates among whites increased for both genders. Analysis of NYSCR data revealed 44 cases of primary IOL. IOL rates per 100,000 person-years for both genders were highest among whites (0.010 for males and 0.012 for females) followed by Asian/Pacific Islanders (0.007 for males and females). Analysis of trends revealed increase in rates for both genders and racial groups.
Conclusions: Our analyses highlight the rarity of VRL and reveal an increase in incidence, particularly among Asian/Pacific Islanders where IOL rates tripled within two decades. Based on incidence rates, 8 cases of IOL (with a subset being VRL) are expected in 4 years in NYS. Our observation of 10 cases of VRL in 4 years in our practices in NYC is unanticipated. The sole common environmental factor we identified was residence in regions proximal to the Chernobyl nuclear disaster.
Citation Format: Sanford Kempin, Paul Finger, Robert Gale, John Rescigno, Jeffrey Rubin, Walter Choi, Rebecca Fisher, Alexander Aizman, Ilona Genis, Roxana Moslehi* (Corresponding Author). A case series of vitreoretinal lymphoma in New York City with exposure to Chernobyl nuclear disaster decades earlier [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 259. doi:10.1158/1538-7445.AM2017-259</jats:p
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