77 research outputs found
The management of infancy and childhood intussusception in a Department of General Surgery
No full textThe management of infancy and childhood intussusception in a Department of General Surgery
No full text[No abstract available
Il nervo laringeo inferiore destro a decorso non ricorrente: un fattore di rischio nella chirurgia della tiroide.
No full textTrisomy 20 in a papillar urothelial carcinoma of ureter.
No full textTo contribute to the knowledge on tumorigenesis and the evolution of urothelial carcinoma of the ureter, we analyzed the clinical, histological, and cytogenetic aspects of a case. Primary cell cultures obtained from tumor specimens showed a trisomy of chromosome 20 where the c-src proto-oncogene, already described in literature as having an important role in the etiology and progression of some tumors, is located. In our case trisomy 20 is the only present marker and for this reason we think that it could play a role in the tumorigenesis of the urothelial carcinoma of the ureter
Flow cytometry: Unravelling the real antimicrobial and antibiofilm efficacy of natural bioactive compounds
No full textFlow cytometry (FCM) provides unique information on bacterial viability and physiology, allowing a real-time early warning antimicrobial and antibiofilm monitoring system for preventing the spread risk of foodborne disease. The present work used a combined culture-based and FCM approach to assess the in vitro efficacy of essential oils (EOs) from condiment plants commonly used in Mediterranean Europe (i.e., thyme EO, oregano EO, basil EO, and lemon EO) against planktonic and sessile cells of food-pathogenic Listeria monocytogenes 56 LY, and contaminant and alterative species Escherichia coli ATCC 25922 and Pseudomonas fluorescens ATCC 13525. Evaluation of the bacterial response to the increasing concentrations of natural compounds posed FCM as a crucial technique for the quantification of the live/dead, and viable but non-culturable (VBNC) cells when antimicrobial agents exert no real bactericidal action. Furthermore, the FCM results displayed higher numbers of viable bacteria expressed as Active Fluorescent Units (AFUs) with a greater level of repeatability compared with outcomes of the plate-count method. Overall, accurate counting of viable microbial cells is a critically important parameter in food microbiology, and flow cytometry provides an innovative approach with high-throughput potential for applications in the food industry as “flow microbiology”
Synergistic Action of Mild Heat and Essential Oil Treatments on Culturability and Viability of Escherichia coli ATCC 25922 Tested In Vitro and in Fruit Juice
No full textThe strengthening effect of a mild temperature treatment on the antimicrobial efficacy of
essential oils has been widely reported, often leading to an underestimation or a misinterpretation of
the product’s microbial status. In the present study, both a traditional culture-based method and Flow
Cytometry (FCM) were applied to monitor the individual or combined effect of Origanum vulgare
essential oil (OEO) and mild heat treatment on the culturability and viability of Escherichia coli in
a conventional culture medium and in a fruit juice challenge test. The results obtained in the culture
medium showed bacterial inactivation with an increasing treatment temperature (55 ◦C, 60 ◦C, 65 ◦C),
highlighting an overestimation of the dead population using the culture-based method; in fact, when
the FCM method was applied, the prevalence of injured bacterial cells in a viable but non-culturable
(VBNC) state was observed. When commercial fruit juice with a pH of 3.8 and buffered at pH 7.0 was
inoculated with E. coli ATCC 25922, a bactericidal action of OEO and a higher efficiency of the mild
heat at 65 ◦C for 5′ combined with OEO were found. Overall, the combination of mild heat and OEO
treatment represents a promising antimicrobial alternative to improve the safety of fruit juice
Cytogenetic and molecular characterization of a de novo 4q24qter duplication: contribution to the associated phenotype.
No full textWe report on a newborn with severe psychomotor retardation, minor anomalies, congenital heart defects, thumb and urogenital abnormalities. Cytogenetic analysis showed a 4q24qter duplication, never described before, as the result of a de novo t(4;14). The extension of the duplicated 4q region was defined by FISH using YAC probes. The breakpoint was localized between 106.3cM (YAC 800f2, D4S1572) and 111 cM (YAC 744e4, D4S1564). Comparing our patient with those previously reported in literature, we observed some features mature frequently reported in these patients: psychomotor retardation, retromicrognathia, low set and/or malformed ears and some more specific traits: congenital cardiac defects, hypoplastic thumb and urogenital abnormalities
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