1,721,121 research outputs found
Aspetti tecnici del prelievo ecoguidato transvaginale di ovociti bovini
No full textNella specie bovina con la messa a punto di tecniche per la produzione di embrioni “in vitro” si è determinata l’esigenza di reperire ovociti in numero cospicuo e nel modo più agevole possibile per sviluppare programmi di riproduzione e di selezione basati sulle tecniche “in vitro”. Nel presente lavoro vengono descritte tre diverse versioni di aghi cosiddetti semimonouso e i risultati del loro impiego in termini di risultati di percentuali di raccolta di ovociti rispetto ai follicoli aspirati e di qualità degli ovociti ottenuti(cumulo ooforo intatto, parzialmente intatto, assente ) I migliori risultati(82,7% di recupero e 76,4% di ovociti di buona qualità ) sono stati ottenuti impiegando aghi monouso per anestesia epidurale (Terumo 18G) opportunamente modificati e connessi ad un tubo prolunga in acciaio (diam. int. 2 mm) con la possibilità di estrazione dal portasonda per eseguire un lavaggio dopo la puntura-aspirazione di ciaschedun follicolo.In this work are described three different disposable needles to be used in bovine ovum pick- up. The best results, 82.7% of collection rate (123 oocytes collected out of 154 follicles diam. ≥4 mm aspirated ) with 76.4% of cumulus intact oocytes were obtained using disposable Terumo 18G spinal needles customized to be attached to a stainless steel tube ( length 60 cm, internal diam. 2 mm) The main features of this long semi –disposable needles are that they are both inexpensive and with the advantage of a very sharp point at every ovum pick-up(OPU) session
[Significance of chorionic somatomammotrophin levels under various conditions of a pregnant woman]
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Localization of calcitonin binding sites in rat central nervous system: evidence of its neuroactivity
No full textThe distribution of calcitonin (CT) binding sites in serial sections of the rat brain and spinal cord has been examined by an 'in vitro' autoradiographic technique using a radioisotope-sensitive sheet film and [125]salmon CT. Autoradiograms of the diencephalic region had the highest grain density throughout the entire hypothalamus, with the exception of the nuclei ventromedialis, posterior and mammillaris, which were not labeled at all. In the brainstem, large amounts of grains were found in the ventrolateral division of the periaqueductal gray, in the locus coeruleus, in the nucleus tractus spinalis nervi trigemini and in the raphe obscurus, pallidus and magnus, while a widespread and lower grain density was observed in the reticular formation. In the spinal cord the labeling was discretely localized in laminae IV, V and VI of the dorsal horn. The observed distribution of CT binding sites is closely related to the neuroendocrine and analgesic effects of exogenous CT and reinforces the concept of a possible neuromodulatory role proposed for the peptide at brain level
Presence of Aquaporins in the bitch uterus and correlation with serum progesterone levels. Potential roles in uterine water movements during the oestrus cycle and the pregnancy
No full textThe role of birth weight on litter size and mortality within 24h of life in purebred dogs : what aspects are involved?
Full text linkIn humans, scientific evidence emphasizes the role of birth weight on neonatal welfare, morbidity and mortality. In canine species, defining normal ranges of birth weight is a harder issue due to a great morphological variability in size, body weight and breed. The aim of this study was to correlate birth weight with litter size and mortality within 24h of life in 789 pups from 140 litters of purebred dogs and to investigate the aspects that might affect these factors. Birth weight was influenced by maternal size, weight and age (P<0.001). The lightest pups were from toy sized or weighing up to 10kg bitches. Conversely, bitches aged 2-8 years whelped heavier pups than younger and older mothers. Birth weight was also related both to litter size, with heavier pups in smaller rather than in larger litters from medium sized bitches, and breed (P<0.05). Unexpectedly, birth weight did not differ between live born and stillborn pups. However, birth weight was lower in pups dying within 24h of life (P<0.05). High mortality of pups was related both to short pregnancies (P<0.05), also showing lighter litters (P<0.001), and to dystocic parturitions (P<0.001). Litter size was associated with parity, type and number of mating, and length of pregnancy (P<0.001). Low birth weight appears to predispose to early neonatal mortality suggesting a predominant role of the breed rather than size and weight in determining birth weight in pups
Calcitonin binding site distribution in the cat central nervous system: a wider insight of the peptide involvement in brain functions
No full textCalcitonin (CT) binding site distribution has been studied in the cat CNS. The autoradiographic analyses of [125I]-eelCT (ECT) binding showed high density of silver grains in the mesencephalic PAG, in the raphe nuclei and in the dorsal horns, laminae I, IV, V, and VI, where ECT may act to inhibit nociceptive transmission. Other binding-rich areas included the caudatus, the amygdala, the hypothalamus, the substantia nigra, the locus coeruleus and the formatio reticularis mesencephalica. Medium to low density was seen, amongst other areas in the cortex piriformis, the hippocampus, the medial and intralaminar thalamus and the tractus spino-thalamicus. ECT binding site distribution revealed essentially homologous locations in the cat and rat CNS. At difference, the presence of binding in the piriform cortex and in discrete thalamic nuclei suggests a widespread involvement of ECT in a variety of central functions in addition to what already demonstrated
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