111 research outputs found
CUSTOMER SATISFACTION AND LOYALTY IN THE PINGI DANCE SCHOOL
No full textDiplomsko nalogo sem razdelila na dva dela, teoretični in praktični del. V teoretičnem delu sem podrobneje predstavila različne definicije zadovoljstva odjemalcev kot tudi dejavnike, ki najbolj vplivajo na njihovo zadovoljstvo in zvestobo. Če storitev ali izdelek zadovolji odjemalčeva pričakovanja, je ta zadovoljen. Če je zadovoljen s ponudbo podjetja, se tja raje vrača, saj si na ta način prihrani nekaj časa ter zmanjša možnosti nezadovoljstva, saj mu ni treba iskati novega ponudnika. Odjemalci se med seboj razlikujejo tako po starosti kot spolu, izobrazbi, čemu dajejo prednosti, zato mora podjetje svoje odjemalce najprej dobro spoznati, da bi lahko zadovoljilo njihove potrebe. Seveda ni enostavnega »recepta«, kako zadovoljiti odjemalce niti kako jih pridobiti oz. obdržati.
V teoretičnem delu sem tudi predstavila Plesno šolo Pingi in njene glavne dejavnosti. Zanimale so me aktivnosti plesne šole za ohranjanje obstoječih odjemalcev kot tudi načini, kako pridobivajo novince.
V praktičnem delu sem naredila raziskavo s pomočjo anketnega vprašalnika. Prvotne trditve sem preverjala z zastavljenimi vprašanji. Potrdila sem lahko vse štiri trditve. V veliko pomoč mi je bilo dobro poznavanje razmer na trgu plesnih storitev, še posebno delovanje Plesne šole Pingi, v kateri tudi sama poučujem.
Z raziskavo sem ugotovila, da je vsesplošna stopnja zadovoljstva s ponudbo Plesne šole Pingi zelo visoka. Na zadovoljstvo in zvestobo odjemalcev najbolj vplivata plesni pedagog in prijetno vzdušje.
Glede na rezultate raziskave lahko rečem, da ima Plesna šola Pingi zadovoljne in zveste odjemalce, kar kaže na dobro delo znotraj podjetja. Plesna šola Pingi s svojimi zastavljenimi cilji in vizijami je tako upravičila naziv »Dobra plesna šola«.This diploma work is divided into two parts, on the theoretical and on the practical part. In the theoretical part of my diploma work, I will present in detail different definitions of satisfaction and loyalty, as well as the factors which play an important role on customer’s satisfaction and customer’s loyalty. If a product or a service satisfies his/her expectations, such a customer will be satisfied. While pleased with the company’s offer, he/she returns to it more likely,, since it saves one’s time without looking for a substitute and lessens the chance of being unsatisfied to a minimum. Customers are different according to age, sex, education and preference. That is why a company has to know first of all its own customers, as their needs can be appropriate satisfied. Of course, there is no easy “recipe” how to satisfy the existing customers nor how to get or obtain the loyal ones.
In the theoretical part the Dance School Pingi and its main activities are presented as well. I was interested in activities for obtaining the existing customers and the ways how to get the new ones.
In the practical part I made a survey using a questionnaire as a research instrument. My first arguments have been reviewed with the aid of written questions. All four of my arguments could be confirmed. Good knowledge of the situation on the dance market and how the Dance School Pingi operates, being one of its dance teachers namely, was very helpful to me.
The survey shows a very high rate of satisfaction with the complete School’s offer. The most important factors which influence on satisfaction and loyalty are dance teachers and a positive atmosphere in the Dance Sschool Pingi.
According to the results, it could be established, that in the Dance School Pingi there are satisfied and loyal customers, which proves very good work within the company.. The Dance School Pingi, having its own goals and visions, however, justifies the name “the Good Dance School”
The whole chromosome-level genome provides resources and insights into the endangered fish Percocypris pingi evolution and conservation
No full textAbstract Background Percocypris pingi (Tchang) was classified as Endangered on the Red List of China′s Vertebrates in 2015 and is widely distributed in the Upper Yangtze River. Although breeding and release into wild habitats have been performed for this commercially important fish in recent years, low genetic diversity has been found in wild populations. Genomic resources are strongly recommended before formulating and carrying out conservation strategies for P. pingi. Thus, there is an urgent need to conserve germplasm resources and improve the population diversity of P. pingi. To date, the whole genome of P. pingi has not been reported. Results In our study, we constructed the first chromosome-level genome of P. pingi by high-throughput chromosome conformation capture (Hi-C) technology and PacBio long-read sequencing. The assembled genome was 1.7 Gb in size, with an N50 of 17,692 bp and a GC content from circular consensus sequencing of 37.67%. The Hi-C results again demonstrated that P. pingi was tetraploid (n = 98), with the genome consisting of 24-type and 25-type chromosomes. Chr.19 of the 24-type chromosomes in P. pingi resulted from the fusion of chr.19 and chr.22 in zebrafish. The divergence times between 24-type and 25-type chromosomes was around 6.1 million years ago. A total of 25,198 and 25,291 protein-coding genes were obtained from the 24-type and 25-type chromosomes, respectively. The ploidy of P. pingi is an allotetraploid. A total of 8,741 genes of P. pingi were clustered into 4,378 gene families that were shared with 14 other species, and the P. pingi genome had 68 unique gene families. Phylogenetic analyses indicated that P. pingi was most closely related to Schizothorax oconnori, and the genes were clustered on one branch. We identified 166 significantly expanded gene families and 173 significantly contracted gene families in P. pingi. The most enriched positive protein-coding genes, such as Bmp-4, Etfdh, homeobox protein HB9, and ATG3, were screened. Conclusion Our study provides a high-quality chromosome-anchored reference genome for P. pingi and provides sufficient information on the chromosomes, which will lead to valuable resources for genetic, genomic, and biological studies of P. pingi and for improving the genetic diversity, population size, and scientific conservation of endangered fish and other key cyprinid species in aquaculture
Un caso di uveite, trombosi cerebrale, iperomocistinemia e mutazione in eterozigoti 677CÞT per MTHFR: aumentata penetranza fenotipica?
No full textNot available
Histopathological Characteristics and Multi-Omics Analysis of Ocular Pigmentation Defects in Albino <i>Percocypris pingi</i>
No full textPercocypris pingi was listed in the China Vertebrate Red List in 2015, and albino P. pingi exhibits remarkable ocular phenotypes due to melanin synthesis defects, including the deficiency of melanin granules in the iris and retinal pigment epithelium (RPE). However, the regulatory mechanism of pigment loss in the eyes of albino P. pingi has not yet been clarified. This study systematically revealed the potential mechanisms underlying the obstruction of ocular melanin synthesis in albino P. pingi through histopathological analysis, transcriptomics, and proteomics techniques. The results showed that the synergistic effects of abnormal H+ transport mediated by SLC45A2, excessive activation of retinol metabolism, and cytoskeletal transport disorders led to the inhibition of tyrosinase activity and retention of pigment granules, ultimately causing melanin deficiency in the eyes. This study first elucidates the molecular network of ocular albinism in fish from a multi-omics perspective, providing a new perspective for the mechanistic research of pigmentation disorders in vertebrates
Genetic Diversity and Structure Analysis of Percocypris pingi (Cypriniformes: Cyprinidae): Implications for Conservation and Hatchery Release in the Yalong River.
No full textPercocypris pingi is a near threatened cyprinid species, which has suffered a dramatic decline due to anthropogenic factors. As one response to this decline, hatchery release for P. pingi has been conducted in the lower reaches of the Yalong River since 2012. To understand the conservation status of this species and the potential impact of the release of hatchery-reared fish, we studied the genetic diversity and population structure of wild and hatchery populations of P. pingi. Two hatchery populations (Jinping [JPH] and Ya'an [YAH]) and two wild populations (Muli [MLW] and Woluo [WLW]) of P. pingi were analyzed based on microsatellite markers and the mitochondrial DNA control region. The results showed that P. pingi possesses moderate levels of genetic diversity, with observed heterozygosities ranging from 0.657 to 0.770 and nucleotide diversities ranging from 0.00212 to 0.00491. Our results also suggested WLW harbors considerable proportions of genetic diversity in this species and serves as a refuge for P. pingi during anthropogenic disturbance, thus playing an important role for the conservation of P. pingi populations. Microsatellite and mitochondrial markers both indicated close genetic relationships between YAH and MLW, JPH and WLW, respectively. The results to some extent reflected the geographical provenances for original broodstocks of the two hatchery populations, which provide some practical guidance for hatchery release of P. pingi. The existence of remarkable genetic divergence distributed along limited geographical range (approximately 10 kilometers) suggests the two wild populations should be regarded at least as two distinct evolutionary significant units (ESUs) and management units (MUs). Considering reduced intra-population genetic variation in hatchery population for release and significant genetic compositions of the two hatchery populations, some appropriate breeding strategies were proposed to benefit conservation of P. pingi
Effects of Water Temperature on the Growth, Antioxidant Capacity, and Gut Microbiota of <i>Percocypris pingi</i> Juveniles
No full textIt is necessary to determine the optimal temperature for Percocypris pingi growth in recirculating aquaculture systems. To describe the effects of temperature, we evaluated the growth, antioxidant enzyme activity, and gut microbiota structure of P. pingi at different temperatures, including 14, 18, 22, and 26 °C. Results showed that increases in body weight of individuals of the groups subjected to 18 and 22 °C temperatures were considerably higher than those in the groups subjected to temperatures of 14 and 26 °C between 20 and 60 d after the experiment started. Acid phosphatase activity in the liver and kidneys of P. pingi did not differ significantly among the various temperature groups (p > 0.05). A gradual restoration of the alkaline phosphatase and superoxide dismutase activities to variations in the surrounding temperature was observed in the liver and kidney of P. pingi. Interestingly, the water temperature did not affect the α-diversity or composition of the gut microbiota of P. pingi. In conclusion, water temperatures between 14 and 26 °C significantly impacted the growth of P. pingi (p < 0.05) but not the liver and kidney antioxidant capacity or the gut microbiota within 60 d
Acute Toxicity of Mercury Chloride (Hgcl2) and Cadmium Chloride (Cdcl2) on the behavior of freshwater fish, Percocypris Pingi
No full textThe present study was performed to examine acute toxicity of two water-borne metals (Hg and Cd) by static bioassays. Fingerlings Percocypris pingi (P. pingi) were exposed to a range of concentrations of heavy metals and the mortality rate were investigated after 24, 48, 72 and 96h. The median lethal concentration (LC50) was determined with Probit analysis. The LC50 values of mercury chloride for the P. pingi at 24, 48, 72 and 96h were 0.441, 0.347, 0.327 and 0.327 mg/L, respectively. But the LC50 values of cadmium chloride at 24, 48, 72 and 96h were 2.551, 2.341, 1.207 and 0.081mg/L, respectively. The toxicity ranking of the two heavy metals was Hg>Cd. The safe concentration of Hg and Cd were 0.0327 and 0.0081mg/L, respectively
Redescription of Protoopalina pingi Nie, 1935 inhabiting the recta of Hylarana guentheri and Pelophylax nigromaculatus in China
No full textA redescription of Protoopalina pingi Nie, 1935 is presented in this paper to complete Nie’s description at both light and scanning electron microscope levels. These organisms were collected from the recta of the frogs Hylarana guentheri Boulenger, 1882 and Pelophylax nigromaculatus Hallowell, 1861 from Jialing River, Sichuan Province and Honghu Lake, Hubei Province, respectively, in China. This is the first record of its occurrence in H. guentheri and P. nigromaculatus. The body of P. pingi is elongated and somewhat spindle-like in shape, slightly narrowed and bluntly rounded at the anterior extremity, while the posterior end is tapering or sharply pointed. The body surface is thickly flagellated, with the caudal tip being barren. The falx, located at the margin of the anterior end, is composed of a narrow band of kinetosomes. Four round or oval-shaped nuclei, usually arranged in a straight line, are situated in the middle region of the body. Comparisons are made between P. pingi and its congeners
Metabolome and Metagenome Signatures Underlying the Differential Resistance of <i>Percocypris pingi</i>, Crucian Carp, and Yellow Catfish to <i>Ichthyophthirius multifiliis</i> Infection
No full textIchthyophthirius multifiliis poses a significant threat to global aquaculture, yet some fish species exhibit remarkable resistance. This study employed a combined LC-MS-based metabolomics and 16S rRNA gene sequencing approach to investigate the intrinsic mechanisms underlying the differential susceptibility of Percocypris pingi, crucian carp, and yellow catfish. Our results revealed distinct skin molecular and microbial profiles in P. pingi associated with its enhanced resilience. Metabolomic analysis identified a significant upregulation of key antioxidants (L-Glutathione reduced, L-Glutathione oxidized, L-Cysteine-glutathione gisulfide, Uric acid, Histamine, N-Acetylhistamine, and scorbic acid) in P. pingi, most notably L-Glutathione reduced, which was 31- and 59-fold higher than in yellow catfish and crucian carp, respectively. Functional enrichment further highlighted the critical role of enhanced antioxidant capacity (centered on glutathione metabolism) and immune/inflammatory responses in the resistance to I. multifiliis of P. pingi. Concurrently, skin microbiome analysis showed that P. pingi hosted a microbial community distinct from the other two species, with significantly higher α-diversity. Notably, P. pingi skin was significantly depleted of the parasitic bacteria Candidatus_Megaira and Candidatus_Midichloria, which were highly abundant in the susceptible species. Furthermore, predicted metagenomic functions indicated that P. pingi’s microbiota was enriched in fundamental metabolic pathways, whereas the microbiota of crucian carp and yellow catfish was skewed towards disease- and immune-related pathways. In conclusion, our findings demonstrate that the superior resistance of P. pingi to I. multifiliis is likely conferred by a synergistic effect of a robust skin antioxidant capacity (primarily driven by glutathione) and a protective skin microbiome that excludes specific parasites. This study provides novel insights into the multi-faceted mechanisms of disease resistance in fish
Redescription of Protoopalina pingi Nie, 1935 inhabiting the recta of Hylarana guentheri and Pelophylax nigromaculatus in China
No full textA redescription of Protoopalina pingi Nie, 1935 is presented in this paper to complete Nie’s description at both light and scanning electron microscope levels. These organisms were collected from the recta of the frogs Hylarana guentheri Boulenger, 1882 and Pelophylax nigromaculatus Hallowell, 1861 from Jialing River, Sichuan Province and Honghu Lake, Hubei Province, respectively, in China. This is the first record of its occurrence in H. guentheri and P. nigromaculatus. The body of P. pingi is elongated and somewhat spindle-like in shape, slightly narrowed and bluntly rounded at the anterior extremity, while the posterior end is tapering or sharply pointed. The body surface is thickly flagellated, with the caudal tip being barren. The falx, located at the margin of the anterior end, is composed of a narrow band of kinetosomes. Four round or oval-shaped nuclei, usually arranged in a straight line, are situated in the middle region of the body. Comparisons are made between P. pingi and its congeners
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