3,983 research outputs found
Barriers preventing introduction of telemedicine
No full textThe data in this file is the raw data use to generate tables 4, 5 and 6, in the paper Jayasinghe, Dayani, Crowder, Richard and Wills, Gary (2016) Model for the adoption of telemedicine in Sri Lanka. Sage Open, 1-14. (doi:10.1177/2158244016668565).
The data is in cvs format. The data for each of the tables separated by a heading:- Clinician raw data; Hospital staff raw data; and public raw data. These correspond to the tables in the main paper.</span
Sagara Jayasinghe, "The ‘Remains of Faith’: Portuguese-Influenced Ecclesiastical Art and Architecture in the Northern Province of Sri Lanka"
No full textTexte par : Sagara Jayasinghe (Faculty of Architecture, University of Moratuwa, Sri Lanka) Abstract The last region in Sri Lanka to come under the influence of the Portuguese was the Northern Kingdom of Jaffna i.e. the Island of Mannar in 1560 and subsequently, the Peninsula of Jaffna in 1619. It is reported that during this period missionaries of the Portuguese Padroado built a great number of churches in the region. Following the expulsion of the Portuguese by the Dutch in the middle of the..
Chlorotic mottle of bean (Phaseolus vulgaris L.)
No full textFor the past years there have been outbreaks of a disease of bean ( Phaseolus vulgaris L.) in Colombia called bean chlorotic mottle. The etiology of bean chlorotic mottle was not known, but the disease was generally believed to be incited by the same whitefly-transmitted virus that causes variegation in malvaceous plants. The aim of this study was to identify and further characterize the causal agent of bean chlorotic mottle.As from the literature it was not clear which symptoms were characteristic of the disease, firstly a symptom had to be established common to all allegedly bean chlorotic mottle-affected bean plants. This symptom, a sharp mosaic in which the discoloured areas ranged from pale greenish-yellow to bright yellow, was of diagnostic value to detect infected plants in the field. In addition to this sharp mosaic, chlorotic mottle-affected bean plants in the field often showed a host of other symptoms, such as mild mottle, rugosity, leaf curling, malformation, dwarfing, proliferation and witches' broom-like growth. In the same affected population some bean plants showed only the sharp mosaic, whereas others showed additional witches' broom-like growth.Mechanical inoculation experiments conducted with crude sap and partially purified preparations from bean plants exhibiting chlorotic mottle in the field, showed three viruses to be present, viz. bean mild mosaic virus, cucumber mosaic virus and southern bean mosaic virus. By means of differential hosts these three viruses could be separated. Isolates of these viruses were designated BMMV-CIAT, CMV-CIAT and SBMV-CIAT, respectively. Experiments conducted with whiteflies did not reveal the presence of any whitefly-transmitted virus in chlorotic mottle-affected bean plants.BMMV-CIAT, is an isometric particle, 32 nm in diameter, with a single-stranded RNA molecule. The virus is readily transmitted mechanically and its host range is restricted to legumes, viz. Glycine max , Macroptilium spp., Phaseolus spp. and Rhynchosia minima . In P. vulgaris the virus usually causes a mild mosaic which is hardly visible, and in certain cultivars with age, the virus becomes latent. Though affected bean plants grew normally producing healthy- looking pods, flowering and pod formation were usually delayed by about a week under glasshouse conditions. The chrysomelid beetles Diabrotica balteata and Cerotoma facialis were efficient vectors of BMMV-CIAT. The virus is seed-transmitted in beans, percentages ranging from 1.2 to 3.6 in the different cultivars tested. In contrast to many other beetle-transmitted viruses, BMMV-CIAT sediments in sucrose gradients as one particle. The physical and chemical properties of BMMV-CIAT are similar or equal to those of BMMV described by Waterworth et al. (1977). The darkly stained granular material in root cells occasionally observed in the light microscope, proved to be aggregates of virus particles.Cross protection and serological tests showed that CMV-CIAT is related to the CMV-type strain. However, many differences exist between them, the most striking being the ability of CMV-CIAT to cause systemic mosaic in P. vulgaris . The host reactions of CMV- CIAT resembled those of CMV-B 32, an isolate from bean in Spain (Bos and Maat, 1974), but the former had a longer longevity in vitro . Generally, CMV-CIAT induced a yellow mosaic in P. vulgaris , but the symptoms varied greatly in different cultivars. In the cultivars Honduras 46 and Porrillo 1 a sharp mosaic, similar to chlorotic mottle, was produced one month after inoculation with CMV-CIAT None of the bean cultivars and CIAT advanced breeding lines tested were found to be resistant or hypersensitive to CMV-CIAT The virus is transmitted by Aphis gossypii and through seeds of P. vulgaris , Vigna radiata and V. unguiculata 'California Blackeye'. It has poor antigenic properties and is serologically related to CMV-B 32, to a CMV isolate from Yucca (Bouwen et al., 1978) and to the Y-strain of CMV (Scott, 1968). Large inclusions in plant tissues infected with CMV-CIAT could easily be detected in the light microscope.The third virus, SBMV-CIAT, is readily transmitted mechanically. Though the host plant range included mostly legumes, the virus also infected Cucumis sativus 'Ashley'. The virus was more harmful to P. acutifolius , in which it caused severe top necrosis, than to P. vulgaris . Among the cultivars and CIAT advanced breeding lines of P. vulgaris , hypersensitive hosts were found. The virus induced a bright yellow mosaic in G. max . Besides P. vulgaris 'Pinto U.I. 650' other suitable assay hosts for SBMV-CIAT were found viz. Mucuna pruriens and M. utilis which reacted with pin-point necrotic local lesions to the virus. SBMV-CIAT is seed transmitted, the percentages ranging from 3.6 to 33.6 depending on the bean cultivar. Thephysical and chemical properties of SBMV-CIAT are similar or equal to the SBMV bean strain, except for the guanine content of the nucleic acid.An experiment conducted to find out the effect of the above- mentioned viruses alone or in combination with each other on bean plants, indicated that symptoms resembling chlorotic mottle could be reproduced in bean plants by mechanical inoculation with CMV-CIAT alone or in combination with the other two viruses. The vast range of other symptoms sometimes exhibited by bean plants with chlorotic mottle in the field, could also be reproduced by mechanically inoculating the viruses in different combinations at different times after planting of seeds. Witches' broom-like symptoms were produced in bean plants inoculated on their primary leaves with a mixture containing SBMV-CIAT with BMMV-CIAT or with CMV-CIATFrom the present study it is clear that bean chlorotic mottle, as described in literature, is a composite disease caused by a combination of BMMV-CIAT, CMV-CIAT and SBMV-CIAT in which CMV-CIAT is responsible for the sharp mosaic and the other two viruses together are responsible for the additional symptoms like rugosity, leaf curling, witches' broom-like growth etc. Efficient vectors of these viruses are present in the bean fields. Though data are not available for the population distribution of aphids, chrysomelid beetles are present throughout the year in the CIAT fields. Seed transmission of the virus is of considerable epidemiological significance. Infected seeds are vehicles for long distance transport and survival from one season to another, especially in case of BMMV-CIAT, and BMMV-CIAT which have a narrow host range. The diseases caused by the three viruses might be controlled by reducing the number of infection sources and by limiting the spread of the viruses
Engineering Multi-Layered Encapsulations for Combinatorial Stem Cell Biology
No full textElectrospray techniques have become established in the life sciences for uses
from cell encapsulation (Chang, 1964) to directed cell placement in more recent
times (Jayasinghe et al., 2006a). During electrostatic encapsulation a conducting
fluid in a needle connected to a high voltage power supply is charged and then
drawn towards a grounded electrode by an electric field resulting in spraying.
Cells and other materials can be encapsulated by suspending them in an alginate
solution and electrospraying directly into a solution containing of a crosslinking
agent, most commonly calcium chloride. This technique can be used to directly
process and encapsulate many different types of materials (Jayasinghe and
Townsend-Nicholson, 2006, Jayasinghe, 2007, Patel et al., 2008). This research
adapts this technology further and progresses it by creating structures with
multiple layers over an extended period with fluorescent markers contained
within the layers, which are created through chemical adsorption. This allows
the encoding information for the use in combinatorial stem cell biology where
instead of individual experiments a large number of permutations are explored
simultaneously. The research covers various parameters governing the
encapsulation and layering processes as well as the biological functionality and
integration as a tool for combinatorial stem cell cultures. The novel
encapsulation and encoding technique presented here has a number of
advantages over the currently available technology and has been filed as patent
PCT/EP2010/006459
(A) Study on the role of tax amnesties in personal income tax compliance in Sri Lanka
No full textThesis(Master) --KDI School:Master of Public Policy,2010masterpublishedby R. M. Jayasinghe
A model of the factors that influence the implementation of a telemedicine solution in Sri Lanka
No full textIn Sri Lanka, urban hospitals offer specialized healthcare services, while rural hospitals have limited services and normally offer only basic hospital facilities. Therefore people in rural areas have to travel relatively long distances to urban hospitals which offer specialized healthcare. This is expensive, while the poor transportation systems in rural areas make travel to urban hospitals time-consuming often involve an overnight stay.The aim of this research is to improve access to healthcare by adopting telemedicine in rural areas where the patient and the clinician in the rural hospital can contact the consultant in the urban hospital using audio, video and data communication methods for specialized healthcare services.The methods used were questionnaires and interviews in the exploratory study, which had small number of participants (83) covering three regions, followed by the main survey which had large number of participants (225) and ten regions. A model for adoption of a telemedicine system in Sri Lanka was developed from the analysis of the literature and the exploratory study. The exploratory study was conducted in three districts of Sri Lanka involving clinicians, hospital staff and the general public from both rural and urban areas, and Health Ministry officials, medical directors and consultants. The major study was conducted in ten districts and on two islands of Sri Lanka to identify the factors in the model which influenced the adoption of telemedicine.The findings of the main survey clearly indicate how important the telemedicine solution is to the rural population. The results also show that hospital staff and the general public were very much in favour of adopting telemedicine. The results also indicate that both clinicians and hospital staff believe that staff involvement, and introducing new policies and standards, will influence the adoption of telemedicine. Clinicians believe that Internet connectivity and the equipment used will not have an effect on introducing telemedicine.This model will be beneficial when implementing a telemedicine system into rural areas of Sri Lanka, which will provide patients with access to specialized healthcare services
Model: Bioinformatics Analysis Suggests That SE_1780 Protein From Staphylococcus Epidermidis May Be a Member of the Fph Family of Lipases
No full textPyMol session file containing the overlay of PDB structures for the POI (3FLE) and B. subtilis Lipase A (1I6W)
Lefschetz fixed point theory and morse inequalities on stratified pseudomanifolds
No full textSubmission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-02-04 without embargo termsThe student, Gayana Jayasinghe, accepted the attached license on 2024-07-06 at 12:55.The student, Gayana Jayasinghe, submitted this Dissertation for approval on 2024-07-06 at 13:06.This Dissertation was approved for publication on 2024-07-11 at 07:31.DSpace SAF Submission Ingestion Package generated from Vireo submission #20970 on 2025-02-04 at 21:04:19In this dissertation, we develop a new framework to understand Lefschetz fixed point theorems and Morse inequalities for elliptic complexes on stratified pseudomanifolds. We prove generalizations of the Atiyah-Bott-Lefschetz fixed point theorem and construct Witten instanton complexes for various operators on such spaces, comparing and contrasting versions for different elliptic complexes and versions for other cohomology theories, and studying applications in mathematics and physics
Dataset: Bioinformatics Analysis Suggests That SE_1780 Protein From Staphylococcus Epidermidis May Be a Member of the Fph Family of Lipases
No full textStructural similarity of POI to proteins of known structure as determined by the DALI server
Santhanadeepaya: Heritage catholic churches of the Kalpitiya peninsula
No full textThe book is a historical and architectural survey of the Catholic churches of the Kalpitiya peninsula which is located in the North-Western province of Sri Lanka. While analysing in detail the historical evolution of art and architecture of the centre of devotion of the peninsula; the St Anne’s Shrine, Talawila, it also traces the diversity of design in other churches of the peninsula. The book also narrates the often turbulent history of the unfolding and institutional growth of Catholicism in the Kalpitiya peninsula
- …
