4 research outputs found
The E subunit of photosystem I is not essential for linear electron flow and photoautotrophic growth in Arabidopsis thaliana
No full textPSI-E is part of the stromal side of photosystem
I (PSI). In Arabidopsis thaliana, the two nuclear genes
PsaE1 and PsaE2 code for PSI-E, and transcripts of PsaE1
are markedly more abundant than PsaE2 transcripts. Stable
null alleles of the two PsaE genes, psae1-3 and psae2-1,
were identiWed and characterised. The psae2-1 mutant
exhibited wild-type like PSI-E abundance and photosynthetic
performance, whereas in the psae1-3 mutant PSI-E
accumulation was decreased by 85%, together with an
impaired thylakoid electron Xow and plant growth rate. The
psae1-3 psae2-1 double mutant totally lacked PSI-E but
was still able to grow photoautotrophically, implying that
PSI-E is not essential for PSI accumulation and thylakoid
electron flo
Impaired photosystem I oxidation induces STN7-dependent phosphorylation of the light-harvesting complex I protein Lhca4 in Arabidopsis thaliana
Get PDFReduction of the plastoquinone (PQ) pool is
known to activate phosphorylation of thylakoid proteins. In
the Arabidopsis thaliana mutants psad1-1 and psae1-3, oxidation
of photosystem I (PSI) is impaired, and the PQ pool
is correspondingly over-reduced. We show here that, under
these conditions, the antenna protein Lhca4 of PSI becomes
a target for phosphorylation. Phosphorylation of the mature
Lhca4 protein at Thr16 is suppressed in stn7 psad1 and stn7
psae1 double mutants. Thus, under extreme redox conditions,
hyperactivation of thylakoid protein kinases and/or
reorganization of thylakoid protein complex distribution
increase the susceptibility of PSI to phosphorylation
Experimental research on the domestic ORC micro power plant with a commercial biomass boiler
No full textThe purpose of the work was to experimentally evaluate the operation of the domestic ORC micro power plant that uses a commercial biomass boiler fueled with wood pellets. The boiler, with a maximum thermal power output of 45kWt, uses a heating jacket and thermal oil as a working medium. The prototypical domestic ORC micro power plant was equipped with a multistage radial-flow microturbine that can generate electricity (2.5kWe at a rotational speed of 24,000 rpm). The microturbine is a key component of the turbogenerator, which was manufactured in oilfree technology. The turbogenerator’s high-speed bearings are lubricated with the low-boiling medium’s vapour. The HFE7100 fluid was used as a working medium in the ORC installation. The paper discusses the thermodynamic conditions to be met for effective operation of the boiler and the results of experimental research. The operating characteristics of the ORC installation and the biomass boiler were presented. Problems that occurred while testing the micro-cogeneration power plant with the boiler and their impact on the electric and thermal efficiency of the cycle were discussed
Experimental research on the domestic ORC micro power plant with a commercial biomass boiler
No full textThe purpose of the work was to experimentally evaluate the operation of the domestic ORC micro power plant that uses a commercial biomass boiler fueled with wood pellets. The boiler, with a maximum thermal power output of 45kWt, uses a heating jacket and thermal oil as a working medium. The prototypical domestic ORC micro power plant was equipped with a multistage radial-flow microturbine that can generate electricity (2.5kWe at a rotational speed of 24,000 rpm). The microturbine is a key component of the turbogenerator, which was manufactured in oilfree technology. The turbogenerator’s high-speed bearings are lubricated with the low-boiling medium’s vapour. The HFE7100 fluid was used as a working medium in the ORC installation. The paper discusses the thermodynamic conditions to be met for effective operation of the boiler and the results of experimental research. The operating characteristics of the ORC installation and the biomass boiler were presented. Problems that occurred while testing the micro-cogeneration power plant with the boiler and their impact on the electric and thermal efficiency of the cycle were discussed
