1,721,013 research outputs found
MXAN: a new software procedure to perform geometrical fitting of experimental XANES (x-ray absorption near edge structure) spectra
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Structure of the Fe-heme in the homodimeric hemoglobin from Scapharca inaequivalvis and in the T72I mutant: an X-ray Absorption Spectroscopy study at low temperature
No full textAtonic force microscopy study of lymphoblastoid cells under 50-Hz 2-mT magnetic field irradiation
No full textAtomic force microscopy (AFM) has been used to observe modifications induced by a 50-Hz 2-mT sinusoidal magnetic field on human B-lymphoblastoid cells (Raji). Cells exposed up to 64 h showed a drastic decrease in cell height during the first 13 h, reflecting the disappearance of microvilli, and a slower variation of the same parameter during the following 51 h together with a widening and loss of the cell's domed shape that corresponds to modifications at the cytoskeleton level
X-ray Absorption Near Edge Spectroscopy of transferrins: a theoretical and experimental probe of the metal site local structure
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pH dependent local structure of ferricytochrome-c studied by x-ray absorption spectroscopy
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UVB radiation induced effects on cells studied by FTIR spectroscopy
No full textWe have made a preliminary analysis of the results about the effects on tumoral cell line (lymphoid T cell line Jurkat) induced by UVB radiation (dose of 310 mJ/cm(2)) with and without a vegetable mixture. In the present study, we have used two techniques: Fourier transform infrared spectroscopy (FTIR) and flow cytometry. FTIR spectroscopy has the potential to provide the identification of the vibrational modes of some of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The second technique has allowed us to perform measurements of cytotoxicity and to assess the percentage of apoptosis. We already studied the induction of apoptotic process in the same cell line by UVB radiation; in particular, we looked for correspondences and correlations between FTIR spetroscopy and flow cytometry data finding three highly probable spectroscopic markers of apoptosis (Pozzi et al. in Radiat Res 168:698-705, 2007). In the present work, the results have shown significant changes in the absorbance and spectral pattern in the wavenumber protein and nucleic acids regions after the treatments
Comparative XANES study of serotransferrin and ovotransferrin at Cu K-edge: evidence of interactions between the metal sites
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