1,721,031 research outputs found
Production of human immunodeficiency virus chronically infected cells grown in protein-free medium
No full textA human T cell line chronically infected with Human Immunodeficiency Virus (HIV) has been adapted to grow in a chemically defined, protein-free medium. Virus particles are produced at rates comparable to those of serum-supplemented cultures; virus preparations free of undesirable proteins can be produced in preparative amounts by simple ultrafiltration procedures and cell culture supernatants can be used as such for the preparation of ELISA solid phases. This material has been used very conveniently for studies concerning characterization of antibodies against HIV-specific proteins, interaction of HIV with complement components and inclusion of human cell-derived proteins into virions; we propose its use as a powerful tool for the structural as well as functional analysis of the virus particle itself
Rapid isolation of pure Complement Factor H from serum for functional studies by the use of a monoclonal antibody that discriminates FH from all the other isoforms
Full text linkSeveral mutations have been identified in the gene coding for Complement Factor H (FH) from patients with atypical Hemolytic Uraemic Syndrome (aHUS), Age-related Macular Degeneration (AMD) and Membranoproliferative Glomerulonephritis (MPGN). These data allow for a precise description of the structural changes affecting FH, but a simple test for specifically assessing FH function routinely is not yet of common use. We have produced and characterised a monoclonal antibody (5H5) which discriminates between FH and the smaller FH-like 1 and FH-related proteins and show here that it specifically binds to FH without detecting the smaller isoforms. We therefore used this mAb for a quick, one-step micro-purification of FH directly from control sera and showed that this affinity chromatography procedure is not disruptive of its cofactor function. We also developed a modified sheep erythrocytes haemolysis test using our antibody and affinity-purified FH. These tests can be used in conjunction for assessing the function of FH purified from patients affected by FH-related diseases. Moreover we used this mAb to develop a FH-specific ELISA test
Presence of autoantibodies against complement regulatory proteins in relapsing-remitting multiple sclerosis
No full textComplement was proposed to play an important role in the onset of Multiple Sclerosis (MS) lesions by inducing physical damage to myelin-producing cells. Every somatic cell is however endowed with a repertoire of membrane-bound molecules which normally down-regulate the complement activation cascade (Regulators of Complement Activation, RCA) and therefore protect cells from complement-dependent lysis. We show here that antibodies against two complement regulatory molecules expressed in the membrane of human cells (CD46 and CD59) are present in sera from relapsing-remitting MS patients in the acute phase, that they are directed against the active site of the RCA molecules and that they inactivate their regulatory function, thus providing a mechanism by which cells of the nervous system might be damaged in a complement-dependent fashion during the acute MS phase. Moreover, we found that most of these sera also contain antibodies reacting with an epitope of the transmembrane glycoprotein of HIV which is conserved in most retroviruses; this may support the hypothesis that self-reacting antibodies might have arisen in these patients as an immune response after retroviral infection or expression of endogenous retroviral proteins
Ricostruzione postoncologica e riabilitazione impianto supportata: risultati clinici e radiografici con follow-up a 15 anni
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Iter diagnostico per la diagnosi delle lesioni radiotrasparenti dei mascellari
No full textObiettivi
Descrivere l’incidenza e le caratteristiche delle lesioni radiotrasparenti dei mascellari al fine di coadiuvare il clinico nel formulare una corretta ipotesi diagnostica e terapeutica.
Materiali e metodi
Viene descritta la classificazione delle lesioni radiotrasparenti dei mascellari, con le loro caratteristiche cliniche e radiografiche e le relative opzioni terapeutiche.
Risultati e conclusioni
Le informazioni che il clinico può ottenere da un attento esame clinico e radiografico di un paziente affetto da una lesione osteolitica possono costituire un’utile guida per un corretto percorso diagnostico. La diagnosi clinica e radiografica sarà comunque presuntiva e solo l’esame istologico consentirà di porre una diagnosi precisa.Objectives
Describing impact and features of radiolucent lesions of the jaws to help clinicians in their diagnostic overview.
Materials and methods
The authors provide the classification of the most common radiolucent lesions of the jaws together with brief indications of the available treatment options.
Results and conclusions
Clinical and radiographic features of radiolucent lesions can be useful guide to start a correct diagnostic pathway. Clinic and radiographic diagnosis is provisional and only histological exam may supply the final diagnosis
Aspetti diagnostici delle fratture condilari in età pediatrica : due casi clinici a confronto
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