95 research outputs found
Development of transgenic wheat plants free of herbicide marker genes and plasmid backbone sequences through “clean gene” technology and positive selection
No full textRegeneration of Italian durum wheat cultivars and transformation with 5+10 HMW glutenin subunits
No full textIl “clean gene” come metodo innovativo per la produzione di piante transgeniche prive di geni di resistenza ad antibiotici o erbicidi
No full textTransformation of Italian durum wheat cultivars with High Molecular Weight Glutenin Subunit genes from the D genome
No full textPhosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation
No full textPhosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation
No full textEXPRESSION OF BEAN PGIP2 UNDER CONTROL OF THE BARLEY LEM1 PROMOTER LIMITS FUSARIUM GRAMINEARUM INFECTION IN WHEAT
No full textFusarium Head Blight (FHB) caused by Fusarium graminearum is one of the most destructive fungal diseases of wheat worldwide. The pathogen infects the spike at flowering time and causes severe yield losses, deterioration of grain quality, and accumulation of mycotoxins. Better understanding of the means of pathogen entry and colonization of floral tissue is crucial to providing effective protection against FHB. Polygalacturonase inhibiting proteins (PGIPs) are cell wall proteins that inhibit the activity of polygalacturonases (PGs), a class of pectin-depolymerizing enzymes secreted by microbial pathogens, including Fusaria. The constitutive expression of a bean PGIP (PvPGIP2) under control of the maize Ubi1 promoter limits FHB symptoms and reduces mycotoxin accumulation in wheat grain [Janni et al. 2008 Molec. Plant Microb. Interact. 21:171]. To better understand which spike tissues play major roles in limiting F. graminearum infection, we explored the use of PvPGIP2 to defend specific spike tissues by expressing it under control of the barley Lem1 promoter [Somleva and Blechl 2005 Cer. Res. Comm. 33:665]. We show here that the expression of PvPGIP2 in lemma, palea, rachis and anthers reduced FHB symptoms caused by F. graminearum compared to symptoms in infected nontransgenic plants. However, the expression of PvPGIP2 only in the endosperm under control of a HMW-glutenin gene promoter did not affect FHB symptom development, indicating that once the pathogen has reached the endosperm, inhibition of the pathogen’s PG activity is not effective in preventing its further spread
Embriogenic callus induction and plant regeneration variability among Italian durum wheat cultivars
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