1,724,942 research outputs found
VDAC2<sup>A172W</sup> mutation limits WEHI-9625 function.
No full text(A) BAX/VDAC2-deficient MEFs expressing HA-VDAC2 or HA-VDAC2A172W were treated with an approximate EC50 dose of BH3-mimetics (10 μm S63845 and 0.1 μm A1331852) and increasing doses of WEHI-9625 as indicated. Cell viability was determined by measuring PI negative cells using flow cytometry. Data are mean ± SD of 3 independent experiments. (B) Hypothetical model for how VDAC2A172 variants may affect BAK stability on the MOM. WEHI-9625 and VDAC2A172I/L stabilise the BAK–VDAC2 interaction such that BAK dissociation from VDAC2, and ensuing apoptotic activity, is restricted. In cells expressing VDAC2A172W, WEHI-9625 is no longer able to stabilise the BAK–VDAC2. A possible molecular explanation for these observations is that VDAC2A172 only partially occupies the region of the hydrophobic groove of BAK identified by our cross linking. WEHI-9625, and medium-sized hydrophobic side chains, may exploit the imperfect fit of A172, enhancing interaction by providing greater surface complementarity. The larger bulk of tryptophan at this position may destabilise the interaction relative to isoleucine or leucine through less ideal surface complementarity, at the same time restricting access to this region for WEHI-9625. The data including flow cytometry gating strategy underlying this figure is available in S1 Data. FACS data files are available in S2 Data. MEF, mouse embryonic fibroblast; MOM, mitochondrial outer membrane; PI, propidium iodide; VDAC2, voltage-dependent anion channel 2.</p
Neoseiulus californicus treated by LC30/LC10/the applicaiton concentration (100μg/mL) of SYP-9625 and CK.txt
No full textThere is the two sex life table data of Neoseiulus californicus fed on Tetranychus cinnabarinus treated by LC30/LC10/CK of SYP-9625 and the two sex life table data of Neoseiulus californicus exposed to the applicaiton concentration (100μg/mL) of SYP-9625 summarized by Yajing Tian and Jingqi Ouyang
Bioassay on different stages of <i>T</i>. <i>cinnabarinus</i> treated with SYP-9625.
No full textBioassay on different stages of T. cinnabarinus treated with SYP-9625.</p
Daily consumption by <i>Neoseiulus californicus</i> exposed to the application concentration of SYP-9625.
No full textDaily consumption by Neoseiulus californicus exposed to the application concentration of SYP-9625.</p
Linked collectors and determiners for: Colecção do Herbário da Universidade Eduardo Mondlane.
No full textNatural history specimen data linked to collectors and determiners held within, "Colecção do Herbário da Universidade Eduardo Mondlane". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/397b0ae4-460e-4680-9625-73ab82f99ac1">https://bionomia.net/dataset/397b0ae4-460e-4680-9625-73ab82f99ac1</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/397b0ae4-460e-4680-9625-73ab82f99ac1">https://gbif.org/dataset/397b0ae4-460e-4680-9625-73ab82f99ac1</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Colecção do Herbário da Universidade Eduardo Mondlane.
No full textNatural history specimen data linked to collectors and determiners held within, "Colecção do Herbário da Universidade Eduardo Mondlane". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/397b0ae4-460e-4680-9625-73ab82f99ac1">https://bionomia.net/dataset/397b0ae4-460e-4680-9625-73ab82f99ac1</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/397b0ae4-460e-4680-9625-73ab82f99ac1">https://gbif.org/dataset/397b0ae4-460e-4680-9625-73ab82f99ac1</a>. Formatted as a Frictionless Data package
Effects of sublethal concentrations and application concentration of SYP-9625 on <i>Tetranychus cinnabarinus</i> (Boisduval) and its natural enemy, <i>Neoseiulus californicus</i> (McGregor)
No full textAbstractObjectiveExploring the effects of acaricides on predatory mites is crucial for the combination of biological and chemical control of pests. In this study, sublethal effects of the new acaricide SYP-9625 on Tetranychus cinnabarinus (Boisduval), effects of application concentration of SYP-9625 on the predatory mite Neoseiulus californicus (McGregor) and functional responses of N. californicus were assessed. The aim of the present study was to evaluate and explore the application of new acaricide SYP-9625 with natural enemy N. californicus.MethodAll the experiments were under laboratory conditions [25 ± 1 °C, 16:8 (L:D) h and 75 ± 5% RH] and based on an age-stage, two-sex life table. The sublethal concentrations against T. cinnabarinus, including LC10 (0.375 μg/mL) and LC30 (0.841 μg/mL) and the application concentration (100 μg/mL) of SYP-9625, were used to evaluate effects on population parameters of N. californicus.ResultT. cinnabarinus females treated with LC30 exhibited significantly reduced net reproductive rates (R0=11.02) of offspring compared to females treated with LC10 (R0=14.96) and untreated females (>R0=32.74). However, the intrinsic rate of increase (rm) and finite rate of increase (λ) of N. californicus indicated that the application concentration of SYP-9625 had no significant negative effect on treated N. californicus eggs (rm=0.277, λ=1.319) compared to the control (rm=0.292, λ=1.338). Additionally, the sublethal concentrations against T. cinnabarinus including LC10 and LC30 showed a dose-dependent mechanism on the predatory mite. SYP-9625 also stimulated the predatory capacity of N. californicus against immobile stages such as eggs and larvae.ConclusionIt is demonstrated that sublethal concentrations of SYP-9625 can inhibit population growth of T. cinnabarinus. And the sublethal concentrations and application concentration had little effect on the population growth of N. californicus. The two advantages showed great commercial potential of this new acaricide. Therefore, N. californicus can manage T. cinnabarinus populations effectively with appropriate SYP-9625 concentrations.</jats:sec
The reproduction and fecundity of <i>Neoseiulus californicus</i> eggs treated with the application concentration of SYP-9625.
No full textThe reproduction and fecundity of Neoseiulus californicus eggs treated with the application concentration of SYP-9625.</p
Population life table parameters of offspring from <i>Neoseiulus californicus</i> females treated with the application concentration of SYP-9625.
No full textPopulation life table parameters of offspring from Neoseiulus californicus females treated with the application concentration of SYP-9625.</p
Effects of sublethal exposure to SYP-9625 on the fecundity and longevity of treated females of <i>Tetranychus cinnabarinus</i>.
No full textEffects of sublethal exposure to SYP-9625 on the fecundity and longevity of treated females of Tetranychus cinnabarinus.</p
- …
