1,738,079 research outputs found
DataSheet_1_miR-939-3p induces sarcoma proliferation and poor prognosis via suppressing BATF2.zip
No full textBackgroundSarcoma is a rare and aggressive malignancy with poor prognosis, in which oncogene activation and tumor suppressor inactivation are involved. Accumulated studies suggested basic leucine zipper transcription factor ATF-like 2 (BATF2) as a candidate tumor suppressor, but its specific role and mechanism in sarcoma remain unclear.MethodsThe expression levels of BATF2 and miR-939-3p were evaluated by using human sarcoma samples, cell lines and xenograft mouse models. Bioinformatics analysis, qPCR, Western blot, cell proliferation assay, overexpression plasmid construction, point mutation and dual luciferase reporter assay were utilized to investigate the role and mechanism of miR-939-3p in sarcoma.ResultsIn this study, we demonstrated that the expression of BATF2 was downregulated in human sarcoma tissues and cell lines. The downregulation of BATF2 was negatively associated with the prognosis of sarcoma patients. Subsequent bioinformatic prediction and experimental validations showed that BATF2 expression was reduced by microRNA (miR)-939-3p mimic and increased by miR-939-3p inhibitor. Additionally, miR-939-3p was upregulated in sarcoma tissues and cells, correlating with a poor prognosis of sarcoma patients. Moreover, miR-939-3p overexpression suppressed sarcoma cell proliferation, which was significantly attenuated by the restoration of BATF2, while siRNA-mediated knockdown of BATF2 aggravated the miR-939-3p-induced promotion of sarcoma cell proliferation. Further computational algorithms and dual-luciferase reporter assays demonstrated that miR-939-3p repressed BATF2 expression via directly binding to its 3’ untranslated region (3’ UTR).ConclusionCollectively, these findings identified miR-939-3p as a novel regulator of BATF2, as well as a prognostic biomarker in sarcoma, and revealed that suppressing miR-939-3p or inducing BATF2 expression may serve as a promising therapeutic strategy against sarcoma.</p
DataSheet_2_miR-939-3p induces sarcoma proliferation and poor prognosis via suppressing BATF2.docx
No full textBackgroundSarcoma is a rare and aggressive malignancy with poor prognosis, in which oncogene activation and tumor suppressor inactivation are involved. Accumulated studies suggested basic leucine zipper transcription factor ATF-like 2 (BATF2) as a candidate tumor suppressor, but its specific role and mechanism in sarcoma remain unclear.MethodsThe expression levels of BATF2 and miR-939-3p were evaluated by using human sarcoma samples, cell lines and xenograft mouse models. Bioinformatics analysis, qPCR, Western blot, cell proliferation assay, overexpression plasmid construction, point mutation and dual luciferase reporter assay were utilized to investigate the role and mechanism of miR-939-3p in sarcoma.ResultsIn this study, we demonstrated that the expression of BATF2 was downregulated in human sarcoma tissues and cell lines. The downregulation of BATF2 was negatively associated with the prognosis of sarcoma patients. Subsequent bioinformatic prediction and experimental validations showed that BATF2 expression was reduced by microRNA (miR)-939-3p mimic and increased by miR-939-3p inhibitor. Additionally, miR-939-3p was upregulated in sarcoma tissues and cells, correlating with a poor prognosis of sarcoma patients. Moreover, miR-939-3p overexpression suppressed sarcoma cell proliferation, which was significantly attenuated by the restoration of BATF2, while siRNA-mediated knockdown of BATF2 aggravated the miR-939-3p-induced promotion of sarcoma cell proliferation. Further computational algorithms and dual-luciferase reporter assays demonstrated that miR-939-3p repressed BATF2 expression via directly binding to its 3’ untranslated region (3’ UTR).ConclusionCollectively, these findings identified miR-939-3p as a novel regulator of BATF2, as well as a prognostic biomarker in sarcoma, and revealed that suppressing miR-939-3p or inducing BATF2 expression may serve as a promising therapeutic strategy against sarcoma.</p
Grammar [939]
No full textGrammar.
This manuscript is now IO Islamic 459 in the India Office collections.
[metadata: Otto Loth, A Catalogue of the Arabic Manuscripts in the Library of the India Office, (volume 1), no. 939 here with further notations and hyperlinks].
939.
459. Size 91/4 in. by 5 in.; foll. 128. Sixteen lines in a page.
A grammatical analysis of the text of the Kâfiyah, styled تركيب الكافية . The author is not mentioned.
It was printed at Calcutta, A.H. 1261. Cf. Stewart׳s Catal. 127, xxvi.
Begins: الكلمة مبتدأ و اللام فيها لتعريف الجنس .
Written in a current hand. Dated Monday, 18th Dhu’l-ḥijjah, 1191. A lacuna on fol. 122v.
Seal of Nuṣrat Jang.
[Tippu.
MicroRNA-939 induces apoptosis in human liver cancer cells by targeting CRKL expression
No full textIntroduction
Over the years, microRNAs (miRs) have been shown to exhibit therapeutic implications in the treatment of cancer. Consistently, this study was designed to examine the expression profile and to decipher the therapeutic implications of miR-939 in human liver cancer.
Material and methods
Expression profiling was performed by qRT-PCR and the proliferation rate of liver cancer cells was monitored by Cell Counting Kit 8 (CCK8) and clonogenic assays. 4,6-diamidino-2-phenylindole (DAPI) staining and annexin V-FITC assays were used to determine the induction of apoptosis. Ultrastructural analysis was performed by transmission electron microscopy (TEM). Western blotting was used to determine protein expression.
Results
miR-939 was found to be significantly (p < 0.05) downregulated in the liver cancerous tissues and cell lines. miR-939 overexpression significantly (p < 0.05) decreased the proliferation and colony formation of liver cancer cells. Ultrastructural analysis, DAPI and annexin V/PI assays showed induction of apoptosis in miR-939 overexpressing liver cancer cells. This was accompanied by upregulation of Bax, p53 and suppression of Bcl-2. In silico analysis showed CRKL to be the target of miR-939. The down regulation of CRKL mimicked the molecular effects of miR-939 in liver cancer cells. Nonetheless, CRKL overexpression abolished the growth inhibitory effects of miR-939 on liver cancer cells.
Conclusions
The present study established the role of the miR-939/CRKL molecular axis in regulation of liver cancer proliferation, and it may also prove an essential therapeutic target for its management
Breast cancer-secreted miR-939 downregulates VE-cadherin and destroys the barrier function of endothelial monolayers
No full textAbstractExosomes-secreted microRNAs play an important role in metastatic spread. During this process breast cancer cells acquire the ability to transmigrate through blood vessels by inducing changes in the endothelial barrier. We focused on miR-939 that is predicted to target VE-cadherin, a component of adherens junction involved in vessel permeability. By in silico analysis miR-939 was found highly expressed in the basal-like tumor subtypes and in our cohort of 63 triple-negative breast cancers (TNBCs) its expression significantly interacted with lymph node status in predicting disease-free survival probability. We demonstrated, in vitro, that miR-939 directly targets VE-cadherin leading to an increase in HUVECs monolayer permeability. MDA-MB-231 cells transfected with a miR-939 mimic, released miR-939 in exosomes that, once internalized in endothelial cells, favored trans-endothelial migration of MDA-MB-231-GFP cells by the disruption of the endothelial barrier. Notably, when up taken in endothelial cells exosomes caused VE-cadherin down-regulation specifically through miR-939 as we demonstrated by inhibiting miR-939 expression in exosomes-releasing TNBC cells.Together, our data indentify an extracellular pro-tumorigenic role for tumor-derived, exosome-associated miR-939 that can explain its association with worse prognosis in TNBCs
Santa Fe (ATSF) 939
No full textA photograph print showing Santa Fe (ATSF) 939, 2-10-2 (BLW) class 900, at coaling dock, Topeka, KS
Reading Railroad (RDG) 939
No full textA photograph print showing the Reading Railroad (RDG) 939, 2-8-0 (Class I-sb) camelback, Pine Grove, PA
El Tlacuache Núm. 939 (2020). 939 Año 19 (2020) junio. El Tlacuache
No full textEl 15 de mayo del presente año, Hugo López-Gatell -Subsecretario de Prevención y Promoción de la Salud del gobierno federal- presentó el programa para la reapertura de actividades en varias partes del país.- Los municipios de la desesperanza; La Montaña de Guerrero en el contexto de la pandemia por Samuel Villela F. - La pandemia vista por los niñ@s por Tania Alejandra Ramírez Rocha. - Reflexiones sobre desequilibrio ecológico y COVID-19 por María Alejandra Elizabeth Olvera Carbajal.BARRERA Hernández, Abel. “El Toro Meco; resistir en las montañas de concreto” Tlachinollan, A. C. 02/05/2020 http://www.tlachinollan.org/opinion-el-toro-meco-resistir- en-las-montanas-de-concreto/ Consultado el 15/05/2020Bellinghausen, Hermann “Pandemia truncó llegada de remesas a La Montaña guerrerense” La Jornada15/04/2020 https: //www. jornada.com.mx/ult imas/poli t ica/2020/04/15/pandemia-trunco-llegada-de-remesas-a-la-montana-guerrerense-5915.html Consultado el 14/05/2020Camacho, Zósimo “Indígenas mexicanos en Nueva York, cercados por el coronavirus” Tlachinollan, A. C. 03/05/2020 http://www.tlachinollan.org/opinion-indigenas-mexicanos-en-nueva-york-cercados-por-el-coronavirus/ Consultado el 10/05/2020CRUZ, Isabel
“Covid-19 y remesas: la tragedia humana puede transformarse en herramienta de desarrollo” El Financiero, 21/04/2020 https://www.elfinanciero.com.mx/opinion/isabel-cruz/covid-19-y-remesas-la-tragedia-humana-puede-transformarse-en-herramienta-de-desarrollo Consultado el 15/05/2020De Dios Palma, Arturo. “Municipios ‘de la esperanza’ no quieren regresar”
El Universal. 18/05/2020 Consultado el 10/06/2020INFOBAE México
“La realidad de los ‘Municipios de la Esperanza’ libres de COVID: casi la mitad han salido de la lista https://www.infobae.com/america/mexico/2020/06/09/la-realidad-de-los-municipios-de-la-esperanzalibres-de-covid-casi-la-mitad-han-salido-de-lalista/ Consultado el 10/06/2020Ocampo Arista Sergio “Afectaciones por Covid-19 llegan hasta La Montaña,
en Guerrero” La Jornada, 29/04/2020 https://www.jornada.com.mx/ultimas/estados/2020/04/29/afectaciones-por-covid-19-llegan-hasta-la-montana-en-guerrero-7185.html Consultado el 14/05/2020Tlachinollan A.C. “Los otros efectos del coronavirus que ‘asfixian’ a los
más pobres de Guerrero” 03/05/2020 http://www.tlachinollan.org/reportaje-los-otros-efectos-del-coronavirus-que-asfixian-a-los-mas-pobres-de-guerrero/ Consultado el 12/05/2020Chacón, Jerry (2015). “Antropología e infancia. Reflexiones sobre los sujetos y los objetos”. Cuicuilco (64): 133-153.Instituto Nacional de Estadística y Geografía. (2010).INEGI. Aguascalientes.Suzán G, Galindo F, Ceballos C. 2000. La importancia de estudio de enfermedades en fauna silvestre. Vet. Méx 31:223-228.
https://www.unenvironment.org/es/noticias-y-reportajes/reportajes/que-estamos-haciendo-para-poner-fin-al-trafico-ilegal-de-vida
https://news.google.com/covid19/map?hl=es-419gl=MX-ceid=MX:es-419
http://biblioteca.semarnat.gob.mx/janium/Documentos/Ciga/Libros2013/CD001601.pd
Santa Fe (ATSF) 939
No full textA photograph print showing Santa Fe(ATSF) 939, 2-10-2 (BLW) class 900, at coaling dock, Topeka, KS. [see 2008.008.1424
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