1,724,433 research outputs found
Mre11-deficient ATLD1 cells are resistant to MK-8776.
No full text<p>A. Whole cell lysates from U2OS, ATLD1 and ATLD1+Mre11 cells were analyzed by western blot for proteins of the MRN complex. B. ATLD1 and ATLD1+Mre11 cells were incubated with 200 nM or 2 µM MK-8776 for 0–24 h, harvested and analyzed by western blotting. The samples incubated for 24 h at 2 µM were also run beside the samples from the opposite cell line to compare signal intensities. C. Cells were incubated with MK-8776 for 24 h or 48 h then allowed to recover in drug-free media, or incubated continuously for 7 days with MK-8776. Total DNA content per well was then assessed as a measure of cell growth. Error bars (shown only in one direction for clarity) represent the standard error of 3 independent experiments. D. ATLD1 and ATLD1+Mre11 cells were incubated for 24 h with and without 1 µM MK-8776. The neutral comet assay was performed and histograms represent cells with DSB. Error bars represent standard error of 2 independent experiments.</p
MK-8776 induces DNA damage and S phase arrest in U2OS cells.
No full text<p>A. Cells were incubated with 200 nM or 2 µM MK-8776 for 0–24 h then analyzed by western blotting for markers of DNA damage. B. Cells were incubated with MK-8776 for 24 h or 48 h then allowed to recover in drug-free media, or incubated continuously for 7 days with MK-8776. Total DNA content per well was then assessed as a measure of cell growth. Error bars (shown only in one direction for clarity) represent the standard error of 3 independent experiments.</p
The role of Mus81 and CDK2 in MK-8776-induced DNA damage.
No full text<p>A. Cells were transfected with siRNA against Mus81 then analyzed by western blotting for markers of DNA damage following a 6-h treatment with MK-8776. B. U2OS cells grown on coverslips were incubated with 1 µM MK-8776, 20 µM roscovitine or both for 6 h then stained with the indicated antibodies and analyzed by confocal microscopy. 100 cells were scored for each condition and are presented as histograms. Error bars represent the standard error of 2 independent experiments. C. U2OS cells were co-incubated with MK-8776 and roscovitine for 6 h and analyzed by western blotting.</p
Chk1 Inhibitor MK-8776 Restores the Sensitivity of Chemotherapeutics in P-glycoprotein Overexpressing Cancer Cells
No full textP-glycoprotein (P-gp), which is encoded by the ATP-binding cassette (ABC) transporter subfamily B member 1 (ABCB1) gene, is one of the most pivotal ABC transporters that transport its substrates across the cell membrane. Its overexpression is one of the confirmed causes of multidrug resistance (MDR), which results in the failure of cancer treatment. Here, we report that checkpoint kinase (Chk) 1 inhibitor MK-8776, a drug candidate in clinical trial, can restore the sensitivity of chemotherapeutics that are substrates of P-gp in KB-C2, SW620/Ad300 cells and human embryonic kidney (HEK)293/ABCB1 cells that overexpress P-gp. MK-8776 remarkably enhanced the cellular [3H]-paclitaxel accumulation and suppressed the efflux function of P-gp without reducing its expression and affecting its cellular localization in cancer cells. Furthermore, MK-8776 (0–40 μM) stimulated the activity of ATPase in P-gp, which was 4.1-fold greater than the control. In addition, MK-8776 formed a cation–π bond and π–π interaction with key residues of the substrate-binding site in P-gp, as indicated by computer-aided molecular docking study. Our study indicated that MK-8776 may significantly enhance the sensitivity of chemotherapeutics that are substrates of P-gp, providing important information for its application in the reversal of MDR
Linked collectors and determiners for: Recent Invertebrates Specimens.
No full textNatural history specimen data linked to collectors and determiners held within, "Recent Invertebrates Specimens". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/5378e1cf-522d-4469-8776-b709579b4a3e">https://bionomia.net/dataset/5378e1cf-522d-4469-8776-b709579b4a3e</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/5378e1cf-522d-4469-8776-b709579b4a3e">https://gbif.org/dataset/5378e1cf-522d-4469-8776-b709579b4a3e</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Chinja, a new genus of spider from the Eastern Arc Mountains of Tanzania (Araneae, Zoropsidae).
No full textNatural history specimen data linked to collectors and determiners held within, "Chinja, a new genus of spider from the Eastern Arc Mountains of Tanzania (Araneae, Zoropsidae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/2870ed42-271b-4ee7-8776-d3804c806fb1">https://bionomia.net/dataset/2870ed42-271b-4ee7-8776-d3804c806fb1</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/2870ed42-271b-4ee7-8776-d3804c806fb1">https://gbif.org/dataset/2870ed42-271b-4ee7-8776-d3804c806fb1</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Potential indicator species of climate changes occurring in Québec, Part 1: the small brown lacewing fly Micromus posticus (Walker) (Neuroptera: Hemerobiidae).
No full textNatural history specimen data linked to collectors and determiners held within, "Potential indicator species of climate changes occurring in Québec, Part 1: the small brown lacewing fly Micromus posticus (Walker) (Neuroptera: Hemerobiidae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/8260db39-8776-4ad6-bd80-46e4e1168bf7">https://bionomia.net/dataset/8260db39-8776-4ad6-bd80-46e4e1168bf7</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/8260db39-8776-4ad6-bd80-46e4e1168bf7">https://gbif.org/dataset/8260db39-8776-4ad6-bd80-46e4e1168bf7</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Potential indicator species of climate changes occurring in Québec, Part 1: the small brown lacewing fly Micromus posticus (Walker) (Neuroptera: Hemerobiidae).
No full textNatural history specimen data linked to collectors and determiners held within, "Potential indicator species of climate changes occurring in Québec, Part 1: the small brown lacewing fly Micromus posticus (Walker) (Neuroptera: Hemerobiidae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/8260db39-8776-4ad6-bd80-46e4e1168bf7">https://bionomia.net/dataset/8260db39-8776-4ad6-bd80-46e4e1168bf7</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/8260db39-8776-4ad6-bd80-46e4e1168bf7">https://gbif.org/dataset/8260db39-8776-4ad6-bd80-46e4e1168bf7</a>. Formatted as a Frictionless Data package
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