1,723,550 research outputs found
VES induces an autophagic response in SGC-7901 cells.
No full text<p>(A) Ultrastructural observations in SGC-7901 cells with transmission electron microscope. (a) Untreated SGC-7901 cells exhibited normal of cytoplasm, cell organelles, and nuclei morphologies. VES-treated SGC-7901 cells showed characteristic ultrastructural morphology of autophagy, (b to c) a significant number of autophagic vacuoles, (d) a giant autophagic vacuole filled with abundant degraded organelles, (e) autophagic vacuoles fused with multivesicular endosome. (a, c, & d, 20 000×; b, 30 000×; e, 12 000×). Typical changes were indicated by arrows. (B) Treatment of SGC-7901 cells with VES for 24 h prominently enhanced formation of autophagic vacuoles and fluorescent intensity, as determined by transfection of GFP-LC3 (C) Protein levels of LC3-I and LC3-II were measured using Western blot analysis. Data was representative of three individual experiments with similar results. (a) SGC-7901 cells were treated with 0, 5, 10, 15, and 20 μg/mL VES for 24 h. (c) SGC-7901 cells were treated with 20 μg/mL VES for 0, 6, 12, 18, and 24 h. (b) and (d) Relative densitometry of protein expression was determined by LC3-II protein densitometry with LC3-I. Actin was used as a loading control.* means <i>p</i> < 0.05 compared with control.</p
A study on the inhibitory effect of matrine on gastric cancer SGC-7901 cells
Get PDFThe objective of this paper was to investigate the antitumour mechanism of action of matrine by studying its inhibitory effect on gastric cancer SGC-7901 cells. SGC-7901 cells were chosen, and cell-killing capacity of matrine on gastric cancer SGC-7901 cells was determined using MTT assay and single PI staining assay. The results showed that matrine had an inhibitory effect on gastric cancer SGC-7901 cells, which was somewhat dose-dependent. The study concluded that matrine has a significant in-vitro inhibitory effect on SGC-7901 tumour cells, influences cell cycle of SGC-7901 cells, and induces their apoptosis.Key words: Matrine; SGC-7901; MTT assa
Growth inhibition of SGC-7901 cells under SPRC treatment.
No full text<p><b>A.</b> Dose-response study of the effects of SPRC on growth inhibition of SGC-7901 cells. <b>B.</b> Effects of SPRC, SAC, PAG, PAG+SPRC and paclitaxel liposome on viability of SGC-7901 cells. <b>C.</b> Effects of SPRC, SAC, PAG, PAG+SPRC and paclitaxel liposome on colony formation in SGC-7901 cells. <b>D.</b> Effects of SPRC, SAC and paclitaxel liposome on migration ability of SGC-7901 cells. Differential cell migration ability was examined by the wound-closure assay. <b>E.</b> Effects of SPRC, SAC and paclitaxel liposome on wound closure speed. Values are expressed as % of control. * represent significant difference between control vs. SPRC, SAC and paclitaxel liposome groups (p<0.01). <sup>#</sup> represent significant difference (p<0.01) between SPRC vs SPRC+PAG group (p<0.01).</p
Effect of RABEX-5 on the growth of SGC-7901 cells <i>in vivo</i>.
No full text<p>(A) Tumor volumes were measured at different time points in mice inoculated with SGC-7901 cells infected with RABEX-5 knockdown lentiviral vector (SGC-7901/KD cells). (B) Tumors harvested from mice 4 weeks post-injection with SGC-7901/KD cells (C) Individual tumor volumes for each mouse after dissection. (D) The final tumor weight was measured at the end of the experiment. (E) Expression of <i>VEGF</i> mRNA in tumors derived from nude mice. (F) Immunohistochemical analysis of VEGF expression in tumors derived from SGC-7901/NC and SGC-7901/KD groups. *<i>P</i><0.05; **<i>P</i><0.01.</p
NS H 7901, hibrid suncokreta (Ukrajina)
No full textNS H 7901, hibrid suncokreta, priznat od strane Ministarstva za poljoprivredu Ukrajine (Міністерство аграрної політики та продовольства України), rešenje Državne komisije za zaštitu biljnih sorti (Державна служба з охорони прав на сорти рослин) br. 17039154 od 11.02.2020. godine, Ukrajina
The expression of HDAC4 in transfected SGC-7901 cells.
No full text<p>HDAC4 expression was determined in SGC-7901 cells transfected with empty vector (NC) or HDAC4 (<b>A</b>) and (<b>B</b>). HDAC4 expression was determined in SGC-7901 cells transfected with siRNA oligos targeting HDAC4 (si-HDAC4) or scrambled siRNA (si-NC) by qRT-PCR and western blot (<b>C</b>) and (<b>D</b>) (n = 4). Data were expressed as mean ± S.E.M. <i>***P<</i>0.001.</p
Floating cells with stem cell properties in gastric cell line SGC-7901
No full textAims To obtain floating spheres from the adherent gastric cancer cell line SGC-7901 and to analyze the properties of the spheres. Methods Serum-free medium was applied to cultured SGC-7901 cells. Limiting dilution assay, tumor formation assay, microarray analysis, and real-time fluorescent quantitative PCR were used to test the stem cell properties of the spheres. Results A subpopulation of SGC-7901 cells formed floating spheres in serum-free medium. These cells showed enhanced tumorigenic ability and also highly expressed certain stem-cell-associated proteins. Conclusions We successfully propagated floating spheres with stem cell properties in the SGC-7901 gastric cell line. </jats:sec
Study of Esculetin-Induced Apoptosis in Human Gastric Carcinoma SGC-7901 Cells
No full textIn order to investigate the effect and its mechanism of esculetin on human gastric carcinoma SGC-7901 cells, the effect of esculetin on human gastric carcinoma SGC-7901 cells were investigated by MTT method in vitro studies. Then the morphological feature changes of apoptosis cells with esculetin of different concentration by transmission electron microscope were observed and the levels of Cty-c in SGC-7901 cells line using FCM method were analyzed. The results revealed that the Esculetin significantly inhibited the growth of human gastric carcinoma SGC-7901 cells and greatly decreased its growth rate with the esculetin concentration increased, and then the morphological feature changes of apoptosis cells had been observed. After 24h, esculetin could make that the concentration of Cty-c in endochylema and Caspase-3 were all increased. Thus the study showed that esculetin remarkably inhibited the growth of human gastric carcinoma SGC-7901 cells and induce its apoptosis.</jats:p
[Duluth Missabe & Northern Ry., Engine Drawing Card, Sketch No. 7901]
No full textThis engine drawing card was created for Duluth Missabe & Northern Railway, Class 10-50 F. Section N-10 F. Sketch 7901. Copy Spec. C-9695
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