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ABT-737-induced caspase-3 cleavage in human platelets is not affected by actin depolymerization.
No full textA. Washed human platelets were incubated in the absence (-) or presence (+) of the actin depolymerizing drug cytochalasin D, and in the absence or presence of 1 μM ABT-737, for the indicated times. Equal amounts of platelet lysate were resolved by SDS-PAGE; immunoblots were probed for pro-caspase-3 and cleaved caspase-3. Beta-actin (β-actin) is shown as a loading control. B. Bar graph depicts caspase-3 cleavage (normalized to beta-actin levels) induced by 1 μM ABT-737 in the absence (ABT-737, white bars) or presence (ABT-737+CytoD) of cytochalasin D. Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors. C. Immunoblots represent pro-caspase-3 and cleaved caspase-3 levels in platelets treated with 10 μM ABT-737, in the presence or absence of cytochalasin D. Beta-actin (β-actin) is shown as a loading control. D. Bar graph depicts caspase-3 cleavage (normalized to beta-actin levels) induced by 10 μM ABT-737 in the absence (ABT-737, white bars) or presence (ABT-737+CytoD) of cytochalasin D. Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors.</p
ABT-737-induced caspase-3 cleavage in human platelets is not affected by the induction of actin polymerization.
No full textA. Washed human platelets were incubated in the absence (-) or presence (+) of the actin polymerizing drug jasplakinolide, and in the absence or presence of 1 μM ABT-737, for the indicated times. Equal amounts of platelet lysate were resolved by SDS-PAGE; immunoblots were probed for pro-caspase-3 and cleaved caspase-3. Beta-actin (β-actin) is shown as a loading control. B. Bar graph depicts caspase-3 cleavage (normalized to beta-actin levels) induced by 1 μM ABT-737 in the absence (ABT-737, white bars) or presence (ABT-737+Jasp) of jasplakinolide. Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors. C. Immunoblots represent pro-caspase-3 and cleaved caspase-3 levels in platelets treated with 10 μM ABT-737, in the presence or absence of jasplakinolide. Beta-actin (β-actin) is shown as a loading control. D. Bar graph depicts caspase-3 cleavage (normalized to beta-actin levels) induced by 10 μM ABT-737 in the absence (ABT-737, white bars) or presence (ABT-737+Jasp) of jasplakinolide. Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors.</p
ABT-737-induced mitochondrial depolarization and PS externalization in human platelets are not affected by actin depolymerization.
No full textA. Mitochondrial depolarization was measured in washed human platelets using the JC-1 dye. Bar graph depicts the loss of mitochondrial membrane potential in platelets treated with DMSO vehicle alone (DMSO, white bars), with 1 μM ABT-737 (ABT-737, blue bars) or with 1 μM ABT plus the actin depolymerizing agent cytochalasin D (ABT-737+CytoD, pink bars). Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors. B, C. Phosphatidylserine (PS) exposure was measured in washed platelets using the Annexin V dye. Bar graphs depict PS exposure in platelets treated with DMSO vehicle alone (DMSO, white bars), with 1 μM ABT-737 (ABT-737, blue bars) or with 1 μM ABT plus the actin depolymerizing agent cytochalasin D (ABT-737+CytoD, pink bars). Data are expressed both in terms of mean fluorescence intensity (B) and as a percentage (%) of PS-positive platelets (C). Data are mean ± SEM, analyzed by using 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors. D. Bar graph depicts the loss of mitochondrial membrane potential in platelets treated with DMSO vehicle alone (DMSO, white bars), with 10 μM ABT-737 (ABT-737, blue bars) or with 10 μM ABT plus the actin depolymerizing agent cytochalasin D (ABT-737+CytoD, pink bars). Data are mean ± SEM, analyzed 2-way ANOVA and Bonferroni post-hoc multiple comparison tests and represent a minimum of 3 independent experiments using blood from different donors. E, F. Bar graphs depict PS exposure in platelets treated with DMSO vehicle alone (DMSO, white bars), with 10 μM ABT-737 (ABT-737, blue bars) or with 10 μM ABT plus the actin depolymerizing agent cytochalasin D (ABT-737+CytoD, pink bars). Data are expressed both in terms of mean fluorescence intensity (E) and as a percentage (%) of PS-positive platelets (F). Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors.</p
ABT-737-induced mitochondrial depolarization and PS externalization in human platelets are not affected by the induction of actin polymerization.
No full textA. Mitochondrial depolarization was measured in washed human platelets using the JC-1 dye. Bar graph depicts the loss of mitochondrial membrane potential in platelets treated with DMSO vehicle alone (DMSO, white bars), with 1 μM ABT-737 (ABT-737, blue bars) or with 1 μM ABT plus the actin polymerizing agent jasplakinolide (ABT-737+Jasp, pink bars). Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors. B, C. Phosphatidylserine (PS) exposure was measured in washed platelets using the Annexin V dye. Bar graphs depict PS exposure in platelets treated with DMSO vehicle alone (DMSO, white bars), with 1 μM ABT-737 (ABT-737, blue bars) or with 1 μM ABT plus the actin polymerizing agent jasplakinolide (ABT-737+Jasp, pink bars). Data are expressed both in terms of mean fluorescence intensity (B) and as a percentage (%) of PS-positive platelets (C). Data are mean ± SEM, analyzed by using 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors. D. Bar graph depicts the loss of mitochondrial membrane potential in platelets treated with DMSO vehicle alone (DMSO, white bars), with 10 μM ABT-737 (ABT-737, blue bars) or with 10 μM ABT plus the actin polymerizing agent jasplakinolide (ABT-737+Jasp, pink bars). Data are mean ± SEM, analyzed 2-way ANOVA and Bonferroni post-hoc multiple comparison tests and represent a minimum of 3 independent experiments using blood from different donors. E, F. Bar graphs depict PS exposure in platelets treated with DMSO vehicle alone (DMSO, white bars), with 10 μM ABT-737 (ABT-737, blue bars) or with 10 μM ABT plus the actin polymerizing agent jasplakinolide (ABT-737+Jasp, pink bars). Data are expressed both in terms of mean fluorescence intensity (E) and as a percentage (%) of PS-positive platelets (F). Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments using blood from different donors.</p
Supplementary Figure 1 from Vinblastine Rapidly Induces NOXA and Acutely Sensitizes Primary Chronic Lymphocytic Leukemia Cells to ABT-737
No full textPDF file - 62K, Figure S1. JNK is not required for apoptosis induced by the combination of vinblastine plus ABT-737.</p
Maintenance planning optimization for the Boeing 737 next generation aircraft
No full textThis research is aimed at developing a cost-effective maintenance planning and packaging method that will lead to the reduction in direct maintenance costs, yet maintaining the reliability of the Boeing 737 NG aircraft.Industrial Engineering and ManagementAerospace Engineerin
Resolución UNRN N° 737/2009. Autoriza para financiar cobertura de convocatoria becas 2010
No full textFil: Universidad Nacional de Río Negro (U). Universidad Nacional de Río Negro. Río Negro, ArgentinaResolución UNRN N° 737/2009. Autoriza para financiar cobertura de convocatoria becas 2010fals
Supplementary Figure 7 from Modulation of NOXA and MCL-1 as a Strategy for Sensitizing Melanoma Cells to the BH3-Mimetic ABT-737
No full textPDF file - 7.8MB, Effects of ABT-737 on growth of 3D melanoma spheroids derived from C8161/NOXA clone C6 (A) ABT-737 was applied at the concentrations shown for 3 days. Note the dose-dependent inhibition of proliferation and invasion as well as decrease of viable cells (calcein-AM) and increase of dead cells (ethidium bromide). Bar: 500 �m. (B) Untreated spheroids; (C) treated with ABT-737 from day 0 at the concentrations shown with replenishment every 3 days for 10 days or (D) treated likewise from day 7. Bars: 500 �m.</p
Resolución CDEyVE Nº 75/2015. Refrendar la Resolución Rectoral Nº 737/2015.
No full textFil: Consejo de Docencia, Extensión y Vida Estudiantil (D). Universidad Nacional de Río Negro. Río Negro, ArgentinaResolución CDEyVE Nº 75/2015. Refrendar la Resolución Rectoral Nº 737/2015
ABT-737-induced caspase-3 cleavage in mouse platelets is unchanged by the loss of gelsolin.
No full textA. Washed wild-type (WT) and gelsolin-null (Gsn-/-) platelets were treated with DMSO vehicle alone, with 1 μM ABT-737 or with 10 μM ABT, for 60 or 120 minutes as indicated. Equal amounts of lysate were resolved by SDS-PAGE; immunoblots were probed for pro-caspase-3 and cleaved caspase-3. Beta-actin (β-actin) and beta-tubulin (β-tubulin) are shown as loading controls. B. Bar graph depicts caspase-3 cleavage (normalized to beta-tubulin levels) in wild-type (WT, grey bars) and gelsolin-null (Gsn-/-, black bars) platelets were treated with DMSO vehicle alone, with 1 μM ABT-737 or with 10 μM ABT, and for 60 or 120 minutes as indicated. Data are mean ± SEM, analyzed by 2-way ANOVA and Bonferroni post-hoc multiple comparison tests, and represent a minimum of 3 independent experiments.</p
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