1,723,484 research outputs found
Ägypter und Amazonen : eine demotische Erzählung des Inaros-Petubastis-Kreises aus zwei Papyri der Österreichischen Nationalbibliothek (Pap. Dem. Vindob. 6165 und 6165 A) /
No full textTexts of Pap. Dem. Vindob. 6165 and 6165A translated from the Egyptian
pre-mir-6165 overexpression in the Hela cells and detection of Hsa-mir-6165 mature form.
No full text<p>A) Schematic presentation of pre-mir-6165 cloning, overexpression and its RNA adenylation followed by cDNA synthesis, using a universal anchored-oligo-dT primer. For the amplification of precursor, first strand cDNA was PCR amplified using precursor specific F-primer and reverse anchor primer on the oligo-dT tail. For the amplification of mature miRNA, predicted mir-6165 sequence was used as the forward primer. B) Hsa-mir-6165 increased production (200,000x) following transfection of Hela cells with its precursor. In the untransfected cells (U) or scrambled control (M), the level of this miRNA was lower compared to the transfected cells (T). C) Four sequencing result of TA vector clones containing mir-6165 real time PCR products, are compared to the precursor sequence. The sequences between the laboratory added polyA and the upstream vector sequence are considered as mature miRNA. Sequencing of clones #2, #3 and #4 shows that prediction of Hsa-mir-6165 sequence has been correct. Clone#1 also shows the similar sequence plus AGG extra nucleotides which is considered an iso-mir for the miRNA.</p
mir-6165 overexpression in U87 cell line induces apoptosis.
No full text<p>A) PI staining of U87 cells 34 hours post transfection was done to investigate the effect of mir-6165 on cell cycle. A dramatic change was observable toward sub-G1 stage in the cells overexpressing mir-6165 compared to negative controls (a′- d′).B) Annexin-PI staining of the U87 cells overexpressing mir-6165 shows, the most of the cells have entered early apoptosis stage compared to negative control and the result is consistent with PI staining in the previous section (a″- d″). The gate setting distinguished between living (bottom left), necrotic (top left), early apoptotic (bottom right), and late apoptotic (top right) cells. Repeated Measures ANOVA analysis shows that the changes observed in flow cytometry of U87 cells is extremely significant (p<0.05) between negative controls and the cells overexpressing mir-6165 (e″).</p
[H.R.A. Grieser, Engine Drawing Card, Sketch No. 6165]
No full textThis engine drawing card was created for H.R.A. Grieser, Class 6-8 1/3 C. Section H-6 1/3 C. Sketch 6165. Copy Spec. C-2390
Detection of Hsa-mir-6165 in the brain derived cell lines and biopsies.
No full text<p>A) NGFR and mir-6165expression profile in some glioma cell lines is compared to non-glioma NT2 cell line Daoy, 1321N1, U87 (glioma cell lines) and NT2 (non glioma cell line) were used for detection of Hsa-mir-6165 expression. U48 small neucleolar RNA was used as internal control for the amplifications. In glioma cell lines Hsa-mir-6165 expression level was higher than NT2 cell line. B) Relative, Hsa-mir-6165 and its precursor expression levels in various human glioma tissue samples. The expression level of Hsa-mir-6165 in the tumor samples were compared to the lowest grade of tumors. U48 small nucleolar RNA gene (SNORD48) was used for normalizing the expression levels. Error bars indicate standard deviation (SD) of duplicate experiments. Pearson’s test confirmed a positive correlation between NGFR and its intronic miRNA (p = 0.0065). In all of the high grades (HG) tissue samples, the level of NGFR and mir-6165 were higher than the low grad (LG) samples.</p
Prediction of pre-mir-6165 within the 4<sup>th</sup> intron of human NGFR gene.
No full text<p>A) Position of predicted hairpin structure within the human NGFR gene is shown in the 4<sup>th</sup> intorn. This hairpin is predicted to produce Hsa-mir-6165 which is shown as red colored sequence on the stem loop. B) Prediction of Drosha enzyme 5' and 3' cutting sites on the sequence of stem loop by Microprocessor SVM. C) Blat search result shows a strong conservation of Hsa-mir-6165 between human, rhesus, dog and elephant.</p
mir-6165 overexpression in Hela cell line.
No full text<p>A) PI staining of Hela cells overexpressing Hsa-mir-6165 did not show any significant change in the stages of cell cycle after 34 hours post transfection (a′- c′). B) Annexin-PI staining of the Hela cells shown in figures a″- d″. Repeated Measures ANOVA analysis shows that the changes observed in annexin test of Hela cells were not significant between negative controls (scramble) and the test group (e″).</p
Copyright Considerations for HIS 6165: Tools for the Digital Historian
No full textPresentation given to HIS 6165 students on January 24, 2018.
This session covered a variety of copyright considerations when considering digital projects. This included basic aspects of copyright. It focused primarily on copyright considerations for digital historians, including exploring fair use, digital humanities and history projects, creative commons licenses, and other copyright-related topics
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