1,723,316 research outputs found
DA-6034 Induces [Ca2+]i Increase in Epithelial Cells
Full text linkDA-6034, a eupatilin derivative of flavonoid, has shown potent effects on the protection of gastric mucosa and induced the increases in fluid and glycoprotein secretion in human and rat corneal and conjunctival cells, suggesting that it might be considered as a drug for the treatment of dry eye. However, whether DA-6034 induces Ca2+ signaling and its underlying mechanism in epithelial cells are not known. In the present study, we investigated the mechanism for actions of DA-6034 in Ca2+ signaling pathways of the epithelial cells (conjunctival and corneal cells) from human donor eyes and mouse salivary gland epithelial cells. DA-6034 activated Ca2+-activated Cl- channels (CaCCs) and increased intracellular calcium concentrations ([Ca2+]i) in primary cultured human conjunctival cells. DA-6034 also increased [Ca2+]i in mouse salivary gland cells and human corneal epithelial cells. [Ca2+]i increase of DA-6034 was dependent on the Ca2+ entry from extracellular and Ca2+ release from internal Ca2+ stores. Interestingly, these effects of DA-6034 were related to ryanodine receptors (RyRs) but not phospholipase C/inositol 1,4,5-triphosphate (IP3) pathway and lysosomal Ca2+ stores. These results suggest that DA-6034 induces Ca2+ signaling via extracellular Ca2+ entry and RyRs-sensitive Ca2+ release from internal Ca2+ stores in epithelial cells.ope
DA-6034-Induced Mucin Secretion Via Ca2+-Dependent Pathways Through P2Y Receptor Stimulation
No full textPURPOSE:
We evaluated whether DA-6034 is involved in mucin secretion via P2Y receptor activation and/or intracellular Ca(2+) concentration ([Ca(2+)]i) change. Also, we investigated the effect of P2Y receptor inhibitors or Ca(2+) chelators on the DA-6034-induced mucin secretion and [Ca(2+)]i increases.
METHODS:
Effects of DA-6034 on mucin expression in primary, cultured, conjunctival epithelial cells was studied using RT-PCR, Western blot analysis, and periodic acid-schiff (PAS) staining. To evaluate thin film layer thickness generated by mucin and fluid secretion, cells were incubated in DA-6034 with/without P2Y antagonists or extracellular/intracellular Ca(2+) chelators, and were imaged with confocal microscope using Texas Red-dextran dye. In addition, DA-6034-induced Ca(2+)-dependent Cl(-) channels opening was evaluated using perforated patch clamp. Fluo-4/AM was used to measure changes in [Ca(2+)]i induced by DA-6034 in Ca(2+)-free or Ca(2+)-containing buffered condition, as well as P2Y antagonists.
RESULTS:
DA-6034 induced the expression of mucin genes, production of mucin protein, and increase of number of mucin-secreting cells. P2Y antagonists inhibited DA-6034-induced mucin and fluid secretion, which was also affected by extracellular/intracellular Ca(2+) chelators. DA-6034 stimulated Cl(-) channel opening and [Ca(2+)]i elevation. Further, [Ca(2+)]i increases induced by DA-6034 were lacking in either P2Y antagonists or Ca(2+)-free buffered condition, and diminished when endoplasmic reticulum Ca(2+) was depleted by cyclopiazonic acid in Ca(2+)-free buffered condition.
CONCLUSIONS:
This study demonstrated that DA-6034 has a potential to induce mucin secretion via Ca(2+)-dependent pathways through P2Y receptors in multilayer, cultured, human conjunctival epithelial cells.ope
C.N.R. steam engine #6034 hauling passenger train, unknown location
No full textHandwritten on back: "CNR Engine #6034"
Block Card 6034 Dellwood Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch Style | 6034 Dellwood Drive (Toledo, Ohio) | Dwelling | Douglas Meadows Addition (Toledo, Ohio) | Trilby Area (Toledo, Ohio)
Günther Christian Hansen (Éd.), Anonyme Kirchengeschichte (Gelasius Cyzicenus, CPG 6034).
No full textGünther Christian Hansen (Éd.), Anonyme Kirchengeschichte (Gelasius Cyzicenus, CPG 6034). . In: Revue des études byzantines, tome 61, 2003. pp. 269-270
The Effect of DA-6034 on Intestinal Permeability in an Indomethacin-Induced Small Intestinal Injury Model
Full text linkBackground/Aims: DA-6034 has anti-inflammatory activities and exhibits cytoprotective effects in acute gastric injury models. However, explanations for the protective effects of DA-6034 on intestinal permeability are limited. This study sought to investigate the effect of DA-6034 on intestinal permeability in an indomethacin-induced small intestinal injury model and its protective effect against small intestinal injury. Methods: Rats in the treatment group received DA-6034 from days 0 to 2 and indomethacin from days 1 to 2. Rats in the control group received indomethacin from days 1 to 2. On the fourth day, the small intestines were examined to compare the severity of inflammation. Intestinal permeability was evaluated by using fluorescein isothiocyanate-labeled dextran. Western blotting was performed to confirm the association between DA-6034 and the extracellular signal-regulated kinase (ERK) pathway. Results: The inflammation scores in the treatment group were lower than those in the control group, but the difference was statistically insignificant. Hemorrhagic lesions in the treatment group were broader than those in the control group, but the difference was statistically insignificant. Intestinal permeability was lower in the treatment group than in the control group. DA-6034 enhanced extracellular signal-regulated kinase expression, and intestinal permeability was negatively correlated with ERK expression. Conclusions: DA-6034 may decrease intestinal permeability in an indomethacin-induced intestinal injury model via the ERK pathway.This work was supported by a research fund from Hanyang University (HY-2011-C)
Anonyme Kirchengeschichte (Gelasius Cyzicenus, CPG 6034)
No full textBei vorliegendem Dokument handelt es sich um den kritischen Text der 2002 von Günther Christian Hansen herausgegebenen Kirchengeschichte eines Anonymus, die herkömmlich dem Gelasios von Kyzikos (CPG 6034) zugeschrieben war. Geboten wird der griechische Lesetext ohne Einleitung, Apparate und Register
Günther Christian Hansen (Éd.), Anonyme Kirchengeschichte (Gelasius Cyzicenus, CPG 6034).
No full textGünther Christian Hansen (Éd.), Anonyme Kirchengeschichte (Gelasius Cyzicenus, CPG 6034). . In: Revue des études byzantines, tome 61, 2003. pp. 269-270
40. Hansen (G.C.), Anonymus Kirchengeschichte (Gelasius Cyzicenus, CPG 6034)
No full textPouderon Bernard. 40. Hansen (G.C.), Anonymus Kirchengeschichte (Gelasius Cyzicenus, CPG 6034). In: Revue des Études Grecques, tome 115, Juillet-décembre 2002. pp. 850-852
The Effect of DA-6034 on Intestinal Permeability in an Indomethacin-Induced Small Intestinal Injury Model
No full textBackground/AimsDA-6034 has anti-inflammatory activities and exhibits cytoprotective effects in acute gastric injury models. However, explanations for the protective effects of DA-6034 on intestinal permeability are limited. This study sought to investigate the effect of DA-6034 on intestinal permeability in an indomethacin-induced small intestinal injury model and its protective effect against small intestinal injury.Methods : Rats in the treatment group received DA-6034 from days 0 to 2 and indomethacin from days 1 to 2. Rats in the control group received indomethacin from days 1 to 2. On the fourth day, the small intestines were examined to compare the severity of inflammation. Intestinal permeability was evaluated by using fluorescein isothiocyanate-labeled dextran. Western blotting was performed to confirm the association between DA-6034 and the extracellular signal-regulated kinase (ERK) pathway.Results : The inflammation scores in the treatment group were lower than those in the control group, but the difference was statistically insignificant. Hemorrhagic lesions in the treatment group were broader than those in the control group, but the difference was statistically insignificant. Intestinal permeability was lower in the treatment group than in the control group. DA-6034 enhanced extracellular signal-regulated kinase expression, and intestinal permeability was negatively correlated with ERK expression.Conclusion : sDA-6034 may decrease intestinal permeability in an indomethacin-induced intestinal injury model via the ERK pathway
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