1,763,856 research outputs found
The effect of transfection of miR-543-5p on GH3 cell line and rat pituitary cells.
No full text(A/E) Analysis of miR-543-5p levels detected by q-PCR after transfection with miR-543a-5p mimic/inhibitor in GH3 cells. (I/M) Analysis of miR-543-5p levels detected by q-PCR after transfection with miR-543a-5p mimic/inhibitor in rat anterior pituitary cells. Observe apoptosis by flow cytometry after staining GH3 cells and rat anterior pituitary cells which were transfected. (B) miR-543-5p nc group in GH3 cell line. (C) miR-543-5p mimic group in GH3 cell line. (F) miR-543-5p i-nc group in GH3 cell line. (G) miR-543-5p inhibitor group in GH3 cell line. (D/H) The percentage of apoptotic GH3 cells after transfection with miR-543-5p nc/mimic/ i-nc/ inhibitor. (J) miR-543-5p nc group in rat anterior pituitary cells. (K) miR-543-5p mimic group in rat anterior pituitary cells. (N) miR-543-5p i-nc group in rat anterior pituitary cells. (O) miR-543-5p inhibitor group in rat anterior pituitary cells. (L/P) The percentage of apoptotic rat anterior pituitary cells after transfection with miR-543-5p nc/mimic/ i-nc/ inhibitor. Fluorescence labeling was detected after transfection with the nc mimic carrying fluorescence markers in GH3 cells (Q) and rat anterior pituitary cells (R). Each experiment was tested over three times. Data are shown as means ± SD. Independent-samples t test was used to analyse statistical significance, Different letters a and b exhibit differences significant between groups (p< 0.05).</p
Physical properties of sediments at DSDP Holes 78-541 and 78-543
No full textSediment cored within the Barbados subduction complex at Sites 541 and 542 are underconsolidated. Underconsolidation and changes in physical properties of the cored section can be related to excess pore water pressure that equals the lithostatic load at Site 542 and to major thrust faulting observed at Site 541. Apparently, the pore fluids within the subduction complex are absorbing the tectonic shock of underthrusting. Sediment sampled from the reference Site 543 on the adjacent Atlantic Plate are also underconsolidated. However, underconsolidation in Hole 543 is apparently due to the movement of excess nitrogen gas observed deeper in the hole. Excess gas was not observed at Sites 541 and 542
Roles of differential expression of miR-543-5p in GH regulation in rat anterior pituitary cells and GH3 cells
No full textGrowth hormone (GH) is an important hormone released by the pituitary gland that plays a key role in the growth and development of organisms. In our study, TargetScan analysis and the dual luciferase reporter assays were used to predict and screen for miRNAs that might act on the rat Gh1 gene, and we identified miR-543-5p. Then, the GH3 cell line and the primary rat pituitary cells were transfected with miRNA mimic, inhibitor, and siRNA. We detected the Gh1 gene expression and the GH secretion by real-time PCR and ELISAs, respectively, to verify the regulatory effect of miR-543-5p on GH secretion. The results showed that miR-543-5p can inhibit Gh1 mRNA expression and reduce GH secretion. MiR-543-5p inhibitor upregulated Gh1 mRNA expression and increased GH secretion compared with the negative control. In summary, miR-543-5p downregulates Gh1 expression, resulting in a decrease in GH synthesis and secretion, which demonstrates the important role of miRNAs in regulating GH and animal growth and development.</div
Element contents and isotope composition of sediments and basalts from DSDP Site 78-543
No full textTwenty-four piston core sediment samples and 13 sediments and 3 basalts from DSDP Leg 78 Site 543 were analyzed for Sr, Nd and Pb isotopic compositions. The results show sediment with highly radiogenic Pb (206Pb/204Pb up to 19.8) and rather radiogenic Sr and unradiogenic Nd has been deposited in the region since the Cretaceous. The source of this sediment is probably the Archean Guiana Highland, which is drained by the Orinoco River. Pb and Sr isotopic compositions and sediment thickness decrease and 143Nd/144Nd increases northward due to a decrease in turbiditic component. This decrease is partly due to the damming action of basement ridges. Rare earth concentrations in the sediments are somewhat low, due to the abundance of detrital and biogenic components in the sediment and rapid sedimentation rates. Both positive and negative Ce anomalies occur in the surface sediments, but only positive Ce anomalies occur in the Site 543 sediments. It is unlikely that sediment subducted to the source region of Lesser Antilles arc magmas could be the cause of negative Ce anomalies in those magmas.
Isotopic compositions of Site 543 basalts show some effect of contamination by seawater-basalt reaction products and sediments. Beyond this, however, they are typical of “normal” depleted MORB
The expression level of miR-543-5p in different development stages and in various rat tissues.
No full text(A) Analysis of miR-543-5p levels detected by q-PCR in rat different tissues. (B) Analysis of miR-543-5p levels detected by q-PCR detected in two-week-old rats and four-month-old rats. (C) Analysis of Gh1 levels detected by q-PCR detected in 7-day-old, 40-day-old, 90-day-old and 250-day-old rats. (D) Growth curve of the body weight in different ages rats with 7-day-old, 40-day-old, 90-day-old and 250-day-old. Each experiment was tested over three times. Data are shown as means ± SD. One-way ANOVA and independent-samples t test were used to analyse statistical significance (*P<0.05; **P<0.01).</p
Resolución UNRN N° 543/2009. Aprobar la contratación de personal temporario
No full textFil: Universidad Nacional de Río Negro (U). Universidad Nacional de Río Negro. Río Negro, ArgentinaResolución UNRN N° 543/2009. Aprobar la contratación de personal temporariofals
El Tlacuache Núm. 543 (2012). 543 Año 13 (2012) noviembre. El Tlacuache
No full textLa fuerza de la palabra y la razón? de la actualidad, la lucha por los espacios colectivos por Marco Tafolla.-Presencias, analogías y definiciones temporales y de procedencia en la Zona Arqueológica El Tlatoani. La Cerámica Plomiza Tohil como marcador del Horizonte Posclásico Temprano por Raúl Francisco González Quezada, Berenice García Vázquez. -Santos en la fachada del Templo de San Mateo Apóstol en Chalcatzingo por América Malbrán Porto
MiR-543-5p targets the 3’ UTR of <i>Gh1</i> mRNA.
No full text(A) Identification of miRNAs which may target the Gh1 3’UTR. Changes of relative luciferase activity of the pmiR- Gh1-3’UTR-WT vector after the 5 candidate miRNAs were transfected into 293T cells. Measuring the relative luciferase activity when the time of transfection reach 48 hours. The normalized relative Rluc/Luc activity ratio for the negative control was set to 1. (B) The base complementary pairing sequence of miR-543-5p and the Gh1 3′ UTR predicted by TargetScan is marked in red. (C) The target site was mutated from GGCAACT to CCGTTGA. The sequencing map showed the sequence. (D) Examining the relative luciferace activity after co-transfection of the plasmid with the miR-543-5p nc/mimic. The luciferase activity of co-transfection of Gh1-3’UTR WT plasmid and nc group was set to 1 as the negative control. Each experiment was tested over three times. Data are shown as means ± SD. One-way ANOVA was used to assess statistical significance, Different letters a and b exhibit differences significant between groups (p< 0.05).</p
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