1,724,415 research outputs found
Table_1_SNP rs4937333 in the miRNA-5003-Binding Site of the ETS1 3′-UTR Decreases ETS1 Expression.DOC
No full textMutations in and reduced expression of the ETS1 gene may be associated with systemic lupus erythematosus (SLE). Here, we report a replication study to investigate associations of eight ETS1 single-nucleotide polymorphisms in the 3′-untranslated region (3′-UTR) with SLE and their regulation of ETS1 expression in a study population. We found that the rs4937333 T allele was associated with a significantly increased risk of SLE (odds ratio: 1.800, 95% confidence interval: 1.02–3.157, P = 0.040) and with dramatically reduced levels of ETS1 in B cells from SLE subjects. Functionally, the rs4937333 T allele alters the binding affinity between miR-5003 and its ETS1 3′-UTR target, thus enhancing suppression of ETS1 expression. Furthermore, immunoglobulin M-secreting plasmacytes were significantly reduced among B cells with the rs4937333 C allele versus the T allele according to FACS and ELISA. Additionally, miR-5003 expression was higher in B cells than in T cells from SLE patients, and a negative correlation between miR-5003 and ETS1 was found, especially in B cells with the T allele. These findings suggest that the rs4937333 T allele is a risk factor for susceptibility to SLE in the studied population. The rs4937333 T allele may enhance the binding of miR-5003 to ETS1, which probably promotes the involvement of ETS1 in the differentiation of B cells into plasmacytes.</p
Santa Fe (ATSF) 5003
No full textA photograph print showing Santa Fe 5003, 2-10-4 (BLW), class 5001, Topeka, KS
Chicago, Burlington & Quincy (CBQ) 5003
No full textA photograph print showing Chicago, Burlington & Quincy (CBQ) 5003, 2-8-2
Santa Fe (ATSF) 5003
No full textA photograph postcard showing Santa Fe (ATSF) 5003, 2-10-4, on stock and fruit extra, coming down Curtis Hill on new location between Belva and Heman, OK. On new location. 96 cars, 50 mph. 8:30am, Plains Division
Block Card 5003 Yorkshire Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 5003 Yorkshire Drive (Toledo, Ohio) | Dwelling | Crestview Subdivision (Toledo, Ohio
Additional file 1 of EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression
No full textAdditional file 1: Table S1. Primers used in this study. Table S2. Oligonucleotides used in this study. Additional file 2: Fig. S1 The cancer-promoting effect of si-circ_0087429 in cervical cancer can be reversed by the miR-5003-3p inhibitor. SiHa cells were transfected with si-NC, si-circ, si-circ + inhibitor NC or si-circ + miR inhibitor for subsequent detection. a-b. The expression of circ_0087429 and miR-5003-3p in each group was tested by qRT–PCR. c-g. CCK-8, EdU and colony formation assays were used to detect the proliferation ability of each group of cells. Scale bar, 50 μm. h-i. Wound healing assays were used to determine the migration ability of each group of cells. Scale bar, 100 μm. j-k. Transwell invasion assays were used to detect the invasion ability of each group of cells. Scale bar, 50 μm. **p < 0.01, ***p < 0.001 .Additional file 3: Fig. S2 The expression of 8 target genes in cervical tissues based on the GEPIA database. a. OGN, b. SCN7A, c. PGR, d. PTGER3, e. FGF7, f. ADAMTS5, g. LMO3, h. ANK2. *p < 0.05 .Additional file 4: Fig. S3 The tumour suppressor effect of circ_0087429 in cervical cancer can be reversed by si-OGN. HeLa cells were transfected with pcDNA5.1-NC, pcDNA5.1-circ, pcDNA5.1-circ + si-NC or pcDNA5.1-circ + si-OGN for subsequent detection. a-b. The expression of circ_0087429 and OGN in each group was tested by qRT–PCR. c-g. CCK-8, EdU and colony formation assays were used to detect the proliferation ability of each group of cells. Scale bar, 50 μm. h-i. Wound healing assays were used to determine the migration ability of each group of cells. Scale bar, 100 μm. j-k. Transwell invasion assays were used to detect the invasion ability of each group of cells. Scale bar, 50 μm. **p < 0.01, ***p < 0.001, ****p < 0.000
Block Card 5003 St. Aubin Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Shotgun houses | 5003 St. Aubin Drive (Toledo, Ohio) | Dwelling | Crestview Subdivision (Toledo, Ohio
Henricus Livrea, Anonymi fortasse Olympiodori Thebani Blenyomachia (P. Berol. 5003)
No full textde Lannoy Ludo. Henricus Livrea, Anonymi fortasse Olympiodori Thebani Blenyomachia (P. Berol. 5003). In: L'antiquité classique, Tome 49, 1980. pp. 359-360
Henricus Livrea, Anonymi fortasse Olympiodori Thebani Blenyomachia (P. Berol. 5003)
No full textde Lannoy Ludo. Henricus Livrea, Anonymi fortasse Olympiodori Thebani Blenyomachia (P. Berol. 5003). In: L'antiquité classique, Tome 49, 1980. pp. 359-360
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