1,726,278 research outputs found
MOESM8 of Long noncoding RNA LCAT1 functions as a ceRNA to regulate RAC1 function by sponging miR-4715-5p in lung cancer
No full textAdditional file 8: Figure S5. miR-4715-5p affects lung cancer cell proliferation and progression. (A) EdU assay was performed to quantify the proliferation of cells overexpressing miR-4715-5p. (B) Colony formation of cells overexpressing miR-4715-5p. (C) miR-4715-5p silencing promotes lung cancer cell proliferation. (D) Representative images of cells overexpressing miR-4715-5p from transwell migration and invasion assay. (E) Proliferation of Calu1 cells after co-transfected with control si-LCAT1–4# and miR-4715-5p inhibitor was measured using a colony formation assay
MOESM9 of Long noncoding RNA LCAT1 functions as a ceRNA to regulate RAC1 function by sponging miR-4715-5p in lung cancer
No full textAdditional file 9: Figure S6. Relationship of LCAT1/miR-4715-5p/RAC1 in lung cancer tissues. (A, B, C) Pairwise correlations among LCAT1/miR-4715-5p/RAC1 in lung cancer tissues and adjacent normal tissues. The expression level of LCAT1/miR-4715-5p/RAC1 was measured by qPCR. (D) RAC1 expression in Calu1 cells transfected with si-LCAT1and miR-4715-5p inhibitor, respectively, and co-transfected with si-LCAT1and miR-4715-5p inhibitor. RAC1 expression was measured by Western blot
MOESM7 of Long noncoding RNA LCAT1 functions as a ceRNA to regulate RAC1 function by sponging miR-4715-5p in lung cancer
No full textAdditional file 7: Figure S4. The negative correlation between LCAT1 and miR-4715-5p. (A) Western blot analysis of subcellular fraction proteins. (B) Quantification of LCAT1, miR-4715-5p, and RAC1 expression by qPCR in lung cancer cell lines. (C) Quantification of miR-4715-5p expression in the cells overexpressing LCAT1 by qRT-PCR. (D) Quantification of LCAT1 expression by qRT-PCR in cells overexpressing miR-4715-5p. (E, F) Kaplan-Meier survival analysis of overall survival and progression-free survival time in lung cancer patients based on miR-4715-5p expression
High-frequency urban measurements of molecular hydrogen and carbon monoxide in the United Kingdom
No full textHigh-frequency measurements of atmospheric molecular hydrogen (H<sub>2</sub>) and carbon monoxide (CO) were made at an urban site in the United Kingdom (UK) from mid-December, 2008 until early March, 2009. Very few measurements of H<sub>2</sub> exist in the urban environment, particularly within the UK, but are an essential component in the assessment of anthropogenic emissions of H<sub>2</sub> and to a certain extent CO. These data provide detailed information on urban time-series, diurnal cycles as well as sources and sinks of both H<sub>2</sub> and CO at urban locations. High-frequency data were found to be strongly influenced by local meteorological conditions of wind speed and temperature. Diurnal cycles were found to follow transport frequency very closely due to the sites proximity to major carriageways, consequently a strong correlation was found between H<sub>2</sub> and CO mole fractions. Background subtracted mean and rush hour molar H<sub>2</sub>/CO emission ratios of 0.53±0.08 and 0.57±0.06 respectively, were calculated from linear fitting of data. The scatter plot of all H<sub>2</sub> and CO data displayed an unusual two population pattern, thought to be associated with a large industrial area 85 km to the west of the site. However, the definitive source of this two branch pattern could not be fully elucidated. H<sub>2</sub> emissions from transport in the UK were estimated to be 188±39 Gg H<sub>2</sub>/yr, with 8.1±2.3 Tg/yr of H<sub>2</sub> produced from vehicle emissions globally. H<sub>2</sub> and CO deposition velocities were calculated during stable night-time inversion events when a clear decay of both species was observed. CO was found to have a much higher deposition velocity than H<sub>2</sub>, 1.3±0.8&times;10<sup>−3</sup> and 2.2±1.5&times;10<sup>&minus;4</sup> m s<sup>−1</sup> (1&sigma;) respectively, going against the law of molecular diffusivity. The source of this unusual result was investigated, however no conclusive explanation was found for increased loss of CO over H<sub>2</sub> during stable night time inversion events
The NuSTAR view of the non-thermal emission from PSR J0437-4715
No full textWe present a hard X-ray Nuclear Spectroscopic Telescope Array (NuSTAR) observation of PSR J0437-4715, the nearest millisecond pulsar. The known pulsations at the apparent pulse period ~5.76ms are observed with a significance of 3.7σ, at energies up to 20keV above which the NuSTAR background dominates. We measure a photon index Γ = 1.50 ± 0.25 (90 per cent confidence) for the power-law fit to the non-thermal emission. It had been shown that spectral models with two or three thermal components fit the XMM-Newton spectrum of PSR J0437-4715, depending on the slope of the power-law component, and the amount of absorption of soft X-rays. The new constraint on the high-energy emission provided by NuSTAR removes ambiguities regarding the thermal components of the emission below 3 keV. We performed a simultaneous spectral analysis of the XMM-Newton and NuSTAR data to confirm that three thermal components and a power law are required to fit the 0.3-20 keV emission of PSR J0437-4715. Adding a ROSAT-PSPC spectrum further confirmed this result and allowed us to better constrain the temperatures of the three thermal components. A phase-resolved analysis of the NuSTAR data revealed no significant change in the photon index of the high-energy emission. This NuSTAR observation provides further impetus for future observations with the NICER mission (Neutron Star Interior Composition Explorer) whose sensitivity will provide much stricter constraints on the equation of state of nuclear matter by combining model fits to the pulsar's phase-folded light curve with the pulsar's well-defined mass and distance from radio timing observations
Revealing two radio-active galactic nuclei extremely near PSR J0437-4715
No full textNewton's gravitational constant G may vary with time at an extremely low level. The time variability of G will affect the orbital motion of a millisecond pulsar in a binary system and cause a tiny difference between the orbital period-dependent measurement of the kinematic distance and the direct measurement of the annual parallax distance. PSR J0437-4715 is the nearest millisecond pulsar and the brightest at radio wavelengths. To explore the feasibility of achieving a parallax distance accuracy of one light-year, comparable to the recent timing result, with the technique of differential astrometry, we searched for compact radio sources quite close to PSR J0437-4715. Using existing data from the Very Large Array and the Australia Telescope Compact Array, we detected two sources with flat spectra, relatively stable flux densities of 0.9 and 1.0 mJy at 8.4 GHz and separations of 13 and 45 arcsec. With a network consisting of the Long Baseline Array and the Kunming 40-m radio telescope, we found that both sources have a point-like structure and a brightness temperature of ≥107 K. According to these radio inputs and the absence of counterparts in other bands, we argue that they are most likely the compact radio cores of extragalactic active galactic nuclei, rather than Galactic radio stars. The finding of these two radio active galactic nuclei will enable us to achieve a sub-pc distance accuracy with in-beam phase-referencing very-long-baseline interferometric observations and provide one of the most stringent constraints on the time variability of G in the near future
Observations of the millisecond pulsar J0437-4715 at 76 MHz
No full textWe report observations of the millisecond pulsar J0437-4715 at 76 and 90 MHz. These observations give a five-fold increase in the frequency range of measurements of this pulsar and provide the first record of the pulsed emission from any millisecond pulsar below 100 MHz. We find that the radio spectrum of the mean pulsed emission of J0437-4715 is similar to that of many other pulsars with a spectral turnover between 90 and 400 MHz. J0437-4715 differs from most other pulsars with respect to the variation of pulse width with radiation frequency and the relative spectra of pulse components
The macrophage-associated microRNA-4715-3p / Gasdermin D axis potentially indicates fibrosis progression in nonalcoholic fatty liver disease: evidence from transcriptome and biological data
No full textNonalcoholic fatty liver disease (NAFLD) is highly possible to progress to cirrhosis, malignancy, and liver failure through fibrogenesis. The enormous potential of pathogenetic and therapeutic targets in NAFLD has been revealed. This study aimed to explore novel factors potentially indicating or mediating NAFLD progression. Multiple bulk and single-cell RNA sequencing datasets were used, in which landscapes of cell populations were clarified to characterize immune cell infiltration. Significantly high infiltration of macrophages (MPs) was discovered during NAFLD progression. Samples in bulk NASH datasets were regrouped by MP level. Highly differentially expressed genes (DEGs) were identified in the Ctrl vs. NASH comparison, low MP vs. high MP comparison, and the weighted gene co-expression network analysis (WGCNA) clusters. Eight hub genes were identified as promising targets by protein–protein interaction analysis and validated in fibrosis progression, microRNA (miR)–protein interactions were predicted, and the hub genes were verified in a free fatty acid (FFA)-induced macrophage injury model. The results showed that Gasdermin D (GSDMD) was upregulated with fibrosis progression in NAFLD and was associated with macrophage infiltration. In addition, a potential regulator (miR-4715-3p) was correlated with GSDMD. The miR-4715-3p/GSDMD axis potentially modulates macrophage-associated immunity and indicates fibrosis progression in NAFLD.</p
Interstellar Scintillation of PSR J0437−4715 on Two Scales?
No full textAims.We sought to determine the scale of scintillation in the interstellar plasma of PSR J0437−4715. Methods.We used the Very Long Baseline Array to obtain scintillation amplitude and phase data, from dynamic spectra at 327 MHz. Results. We observe two scales of scintillation of pulsar PSR J0437−4715, differing by more than an order of magnitude in scintillation bandwidth. The wider-bandwidth scale of scintillation that we observe indicates less scattering for this pulsar than for other nearby pulsars, other than PSR B0950+08. Key words. turbulence – pulsars: individual (PSR J0437−4715) – Techniques: interferometric 1
Block Card 4715 Vogel Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch Style | Dwelling | 4715 Vogel Drive (Toledo, Ohio) | Elmhurst Park Addition (Toledo, Ohio) | Deveaux Area (Toledo, Ohio) | West Toledo (Toledo, Ohio
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