1,725,301 research outputs found
Reactivity of mAb 4713 with HLA class II molecules.
(A) Purification of mAb 4713-binding proteins from L428 cell lysate by affinity chromatography using mAb 4713. (B) Western blot analysis of the affinity-purified proteins isolated from RAW264.7 or L427 cell lysate using mAb 4713. (C) Flow cytometry analysis of mAb 4713 reactivity with HLA-DP-, HLA-DQ-, or HLA-DR-transfected 293T cells. Cells were incubated with mAb 4713, followed by PE-conjugated rat anti-mouse Igs.</p
Tesa® 4713 Paper Standard
Tesa® 4713 es una cinta de embalaje de uso general basada en un soporte de papel crepado fino y un adhesivo de caucho natural. El soporte de papel de tesa® 4713 está fabricado con papel procedente de bosques bien gestionados y otras fuentes controladas. El sistema adhesivo de caucho natural presenta una buena adhesión a múltiples superficies, así como una buena adherencia inicial.#Cinta#AdhesivoTesa® 4713 is a general purpose packaging tape based on a thin crepe paper backing and a natural rubber adhesive. tesa® 4713 paper backing is made from paper from well-managed forests and other controlled sources. The natural rubber adhesive system has good adhesion to multiple surfaces, as well as good initial adhesion
Block Card 4713 Harbord Drive
This image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 4713 Harboro Drive (Toledo, Ohio) | Dwelling | Monroe Acres (Toledo, Ohio) | Franklin Park Area (Toledo, Ohio) | Trilby Area (Toledo, Ohio)
Block Card 4713 Vermaas Avenue
This image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 4713 Vermaas Avenue (Toledo, Ohio) | Folk House Style | North Toledo (Toledo, Ohio) | Willys Park area (Toledo, Ohio) | Alfred Bailey Addition (Toledo, Ohi
Reactivity and cytotoxic activity of mAb 4713 against lymphoma cell lines.
(A) Flow cytometry analysis of mAb 4713 reactivity analyzed by incubating different lymphoma cell lines with mAb 4713 (4°C; 2 h), followed by Alexa 488-conjugated rat anti-mouse Igs (green histogram). Cytotoxicity was analyzed based on propidium iodide (PI) staining of dead cells (red histogram). (B) Cytotoxic effects of mAb 4713 on IL-2-dependent or IL-2-independent ATL cell lines analyzed by PI staining after incubation with mAb 4713 (37°C; 1 or 2 h). Similar data were obtained in two independent experiments. (C) Kaplan–Meier survival analysis of mAb 4713-treated C.B-17/ICR-SCID mice bearing Raji lymphoma xenografts. The Raji-injected SCID mice (n = 5) were treated with mAb 4713 once (red line) or twice (brown line), or with control IgG once (green line). **p = 0.01 versus isotype control.</p
Block Card 4713 Clover Lane
This image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 4713 Clover Lane (Toledo, Ohio) | Dwelling | Greenbush Knolls Addition (Toledo, Ohio) | Trilby Area (Toledo, Ohio)
Linked collectors and determiners for: A revision of the genus Mesoconius Enderlein (Diptera, Micropezidae, Taeniapterinae).
Natural history specimen data linked to collectors and determiners held within, "A revision of the genus Mesoconius Enderlein (Diptera, Micropezidae, Taeniapterinae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/bdc12bce-f24c-4713-8026-a1aee4193ef5">https://bionomia.net/dataset/bdc12bce-f24c-4713-8026-a1aee4193ef5</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/bdc12bce-f24c-4713-8026-a1aee4193ef5">https://gbif.org/dataset/bdc12bce-f24c-4713-8026-a1aee4193ef5</a>. Formatted as a Frictionless Data package
Microscopy analysis of mAb 4713-induced single cell death shows giant pores.
(A) Light microscopy image showing that the incubation of L428 cells with mAb 4713 caused rapid aggregation and death of single cells after 30 min, as shown by trypan blue exclusion staining. ←, single cell death; ∆, aggregation of cells. Bar: 50 μm (B) Scanning electron microscopy image showing the formation of giant pores on L428 Hodgkin lymphoma cells after a 15 or 30 min incubation with mAb 4713.</p
Mechanism of mAb 4713-induced cell death involving the cytoskeleton.
(A) Impact of chemical inhibitors on the cytotoxic activity of mAb 4713 against L428 and Raji cells. The inhibitors were added 1 or 2 h before the cytotoxicity assay. The percentage of dead cells was determined by trypan blue dye exclusion. **p = 0.01 versus none. ***p = 0.001 versus none. The agents were caspase inhibitors (z-VAD-FMK and z-Asp-DCB), PI-3 kinase inhibitors (wortmannin and LY294002), cytoskeletal inhibitors (cytochalasin D and latrunculin B), necroptosis inhibitor (Necrostatin-1), necrosis inhibitor (IM-54), a cathepsin inhibitor (Cath inib III), and reactive oxygen species scavenger (Tiron). (B) Limiting dilution experiments in which L428 cells were seeded (0.3 cells/well; 96 wells) with 3 μg/ml mAb 4713 or control IgG. After 2 h, the number of live cells per well was counted. **p = 0.01 versus isotype control. ***p = 0.001 versus control IgG. Each value represents the mean ± SD.</p
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