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Aitkin, Harry Augustine, 4401
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/368011Surname: AITKIN
Given Name(s) or Initials: HARRY AUGUSTINE
Military Service Number or Last Known Location: 4401
Missing, Wounded and Prisoner of War Enquiry Card Index Number: 46379178058
Item: [2016.0049.00343] "Aitkin, Harry Augustine, 4401
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Abstract #4401: p22phox-dependent regulation of tuberin function in RCC: role of ERK and Akt pathways
No full textAACR Annual Meeting-- Apr 18-22, 2009; Denver, CO
Mutations in the von Hippel-Lindau (VHL) tumor suppressor gene give rise to hereditary and sporadic clear cell Renal Cell Carcinoma (RCC). In VHL-deficient cells, the redox sensitive signaling molecules Akt and ERK are constitutively active promoting cell growth through HIF-2alpha. Mammalian target of rapamycin (mTOR), an important positive regulator of cell growth, is negatively regulated by the dimer, harmatin and tuberin. Inhibition of tuberin function can occur through post-translational inactivation by ERK and Akt leading to the dissociation of the harmatin/tuberin complex and through Akt-dependent ubiquitination and degradation of tuberin. Activation of mTOR leads to the phosphorylation of eukaryotic initiation factor 4E-binding protein-1 (4E-BP1) and the ribosomal protein S6 kinase 1 (S6K), components essential for mRNA translation. We have previously demonstrated that in VHL-deficient cells, p22phox-based Nox oxidases maintain hypoxia-inducible factor-2alpha protein expression through an Akt-dependent mRNA translational pathway. Here, we show that in VHL-deficient cells, ERK is necessary for maintaining HIF-2alpha through inactivation of tuberin and activation of mTOR downstream targets 4E-BP1 and S6K. Moreover, p22phox acts upstream of ERK and Akt to regulate mRNA translation through tuberin as RNA mediated knockdown of p22phox inhibits ERK and Akt-dependent phosphorylation of tuberin, stabilizes tuberin protein levels and inhibits the phosphorylation of downstream mTOR substrates 4E-BP1 and S6K. Similarly, p22phox over-expression in proximal tubular epithelial cells results in the activation of ERK, Akt, and downstream mTOR targets S6K and 4E-BP1. Importantly, we find that p22phox is over-expressed in over 90% of human RCC compared to adjacent normal tissue, which correlates with increased tuberin phosphorylation and decreased tuberin protein levels with downstream activation of 4E-BP1. These studies suggest that p22phox is a pivotal target for therapeutic strategies to treat RCC.
Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 4401
DEA 4401 Course Syllabus - Fall 10
No full textDEA 4401, Interior Design Studio VII, Course Syllabus by Instructor Rhonda Gilmore, Fall 201
St. Louis & San Francisco (SLSF) "Frisco" 4401
No full textA photograph print showing the St. Louis and San Francisco (SLSF) "Frisco" 4401, 4-8-2, St. Louis, MO
Enhanced Klebsiella pneumoniae Carbapenemase Expression from a Novel Tn <i>4401</i> Deletion
No full textABSTRACT
The
Klebsiella pneumoniae
carbapenemase gene (
bla
KPC
) is typically located within mobile transposon Tn
4401
. Enhanced KPC expression has been associated with deletions in the putative promoter region upstream of
bla
KPC
. Illumina sequences from
bla
KPC
-positive clinical isolates from a single institution were mapped to a Tn
4401
b reference sequence, which carries no deletions. The novel isoform Tn
4401
h (188-bp deletion [between
istB
and
bla
KPC
]) was present in 14% (39/281) of clinical isolates. MICs showed that
Escherichia coli
strains containing plasmids with Tn
4401
a and Tn
4401
h were more resistant to meropenem (≥16 and ≥16, respectively), ertapenem (≥8 and 4, respectively), and cefepime (≥64 and 4, respectively) than
E. coli
strains with Tn
4401
b (0.5, ≤0.5, and ≤1, respectively). Quantitative real-time PCR (qRT-PCR) demonstrated that Tn
4401
a had a 16-fold increase and Tn
4401
h a 4-fold increase in
bla
KPC
mRNA levels compared to the reference Tn
4401
b. A
lacZ
reporter plasmid was used to test the activity of the promoter regions from the different variants, and the results showed that the Tn
4401
a and Tn
4401
h promoter sequences generated higher β-galactosidase activity than the corresponding Tn
4401
b sequence. Further dissection of the promoter region demonstrated that putative promoter P1 was not functional. The activity of the isolated P2 promoter was greatly enhanced by inclusion of the P1-P2 intervening sequence. These studies indicated that gene expression could be an important consideration in understanding resistance phenotypes predicted by genetic signatures in the context of sequencing-based rapid diagnostics.
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Block Card 4401 Lyman Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 4401 Lyman Avenue (Toledo, Ohio) | Cape Cod Style | Walnut Hills Extension (Toledo, Ohio) | Willys Park area (Toledo, Ohio) | West Toledo (Toledo, Ohio
Block Card 4401 Foxglove Road
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 4401 Foxglove Road (Toledo, Ohio) | Dwelling | Harvest Lane Home Sites (Toledo, Ohio) | Franklin Park Area (Toledo, Ohio) | Trilby Area (Toledo, Ohio)
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