1,726,627 research outputs found
Astronomische Nachrichten, nos 3817-3833
No full textR. R. Astronomische Nachrichten, nos 3817-3833. In: Bulletin astronomique, tome 20, 1903. pp. 173-194
Block Card 3833 Ellenridge Lane
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Dwelling | 3833 Ellenridge Lane (Toledo, Ohio) | Ranch houses | Woodley Estates (Toledo, Ohio) | Westgate Area (Toledo, Ohio) | West Toledo area (Toledo, Ohio
Congressional Record S. 3833-4 - Inequitable Treatment of the Indian
Full text linkCongressional Record S. 3833-4 - Inequitable Treatment of the India
Block Card 3833 Burton Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3833 burton Avenue (Toledo, Ohio) | Tudor Cottage style | Almeda Heights Second Addition (Toledo, Ohio) | Willys Park area (Toledo, Ohio) | West Toledo (Toledo, Ohio
Block Card 3833 Airport Highway
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Highland Lanes Miniature Golf (Toledo, Ohio) | Postmodern Style | 3833 Airport Highway (Toledo, Ohio) | commercial buildings | South Toledo Area (Toledo, Ohio
Block Card 3833 S. Beverly Hills Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: 3833 South Beverly Hills Drive (Toledo, Ohio) | Albert J. Coreys Beverly Hills (Toledo, Ohio) | South Toledo Area (Toledo, Ohio) | Colonial Revival Style | Dwellin
4-Thiazolidinone derivative Les-3833 effectively inhibits viability of human melanoma cells through activating apoptotic mechanisms
Full text linkAim To evaluate cytotoxic action of 4-thiazolidinone derivative
Les-3833 and study the mechanisms of its pro-apoptotic
action toward human melanoma cells and human tumor
cell lines of other tissue origin.
Methods The effect of Les-3833 or doxorubicin on the viability
of 9 cell lines was studied using MTT assay, while
human melanoma cells of WM793 line were additionally
examined using light and fluorescent microscopies for
evaluating cytomorphological changes. The Western-blot
and flow cytometric analyses were carried out to study signaling
pathways of melanoma cell cycling and death.
Results Les-3833 was the most efficient against melanoma
cells. Its half maximal inhibitory concentration (IC50)
was 0.22 μg/mL for WM793 cells and 0.3 μg/mL for SK-Mel-
28 melanoma cells. For human lung A549, breast MCF-7,
colon HCT116, and ovarian SKOV3 carcinoma cell lines IC50
was in between 2.5 to >5.0 μg/mL. Les-3833 was relatively
not toxic (IC50 > 5 μg/mL) for human embryonic kidney
HEK293 cells. Results of Annexin V/PI staining of melanoma
cells and activation of caspase 3, PARP, MAPK, and EndoG
protein suggest apoptosis in Les-3833-treated cells. Les-
3833 also induced ROS production in melanoma cells and
their arrest in G0/G1 phase of cell cycle.
Conclusion Novel 4-thiazolidinone derivative Les-3833 is
effective against human melanoma cells in vitro, and such
effect is tumor specific since it is much less pronounced in
human carcinoma and leukemia cells. In melanoma cells
Les-3833 induces apoptosis (morphological changes and
increased pro-apoptotic proteins), ROS production, and arrest
in G0/G1 phase of cell cycle
[Veto of H. 3833, R-77]
No full textThis veto message from Governor Mark Sanford vetoes H. 3833, R-77, a bill that mandates that the Board of Trustees of Cherokee County School District I and the Board of
Commissioners of Public Works of the City of Gaffney be elected at the same time and in the same manner as the Gaffney City Council
Biodistribution and Anticancer Characteristics of Les-3833, A Novel 4-thiazolidinone-Based Lead Compound
No full textRecently, we identified the promising anticancer potential of the synthetic 4-thiazolidinone-based anticancer lead compound Les-3833 which demonstrated tumor-suppressing action in vitro and in vivo. Based on the results of previous studies, the aim of this research was to investigate the cytotoxicity in vitro and the biodistribution in laboratory mice to support the biotherapeutic drug development of Les-3833. Les-3833 (2.5 mg/kg) was intravenously injected into male Balb/c mice. Measurements were performed at 5 min, 15 min, 1 h, 4 h, and 24 h time points in blood plasma, brain, liver, and kidney using high-performance liquid chromatography/tandem mass spectrometry. After the administration of Les-3833, the maximum level of this compound was observed in plasma at 2.08 min. In the brain, the mean maximum concentration of Les-3833 was 7.17 ng/mL at 5 min, while after 15 min, it was not found. In the liver, at 5 min, the maximum concentration was 1190 ng/g. At 15 min, concentration of Les-3833 in the liver decreased by 14.3%; at 6 h by 22.8%; and after 24 h by 64.7%. Its maximum concentration in kidney was 404 ng/g within 5–15 min, at 1 h it decreased by 36.1%, and after 24 h by 49.3%. Thus, Les-3833 was rapidly taken up by different organs from the bloodstream, partially metabolized in the liver, and excreted mainly through the kidneys, while in the brain, a very low concentration could be observed for only a short period of time
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