1,729,276 research outputs found
Image_1_IFN-I inducible miR-3614-5p targets ADAR1 isoforms and fine tunes innate immune activation.jpeg
No full textRegulation of innate immune responses is essential for maintenance of immune homeostasis and development of an appropriate immunity against microbial infection. We show here that miR-3614-5p, product of the TRIM25 host gene, is induced by type I interferon (IFN-I) in several human non-immune and immune cell types, in particular in primary myeloid cells. Studies in HeLa cells showed that miR-3614-5p represses both p110 and p150 ADAR1 and reduces constitutive and IFN-induced A-to-I RNA editing. In line with this, activation of innate sensors and expression of IFN-β and the pro-inflammatory IL-6 are promoted. MiR-3614-5p directly targets ADAR1 transcripts by binding to one specific site in the 3’UTR. Moreover, we could show that endogenous miR-3614-5p is associated with Ago2 and targets ADAR1 in IFN-stimulated cells. Overall, we propose that, by reducing ADAR1, IFN-I-induced miR-3614-5p contributes to lowering the activation threshold of innate sensors. Our findings provide new insights into the role of miR-3614-5p, placing it as a potential fine tuner of dsRNA metabolism, cell homeostasis and innate immunity.</p
Table_1_IFN-I inducible miR-3614-5p targets ADAR1 isoforms and fine tunes innate immune activation.docx
No full textRegulation of innate immune responses is essential for maintenance of immune homeostasis and development of an appropriate immunity against microbial infection. We show here that miR-3614-5p, product of the TRIM25 host gene, is induced by type I interferon (IFN-I) in several human non-immune and immune cell types, in particular in primary myeloid cells. Studies in HeLa cells showed that miR-3614-5p represses both p110 and p150 ADAR1 and reduces constitutive and IFN-induced A-to-I RNA editing. In line with this, activation of innate sensors and expression of IFN-β and the pro-inflammatory IL-6 are promoted. MiR-3614-5p directly targets ADAR1 transcripts by binding to one specific site in the 3’UTR. Moreover, we could show that endogenous miR-3614-5p is associated with Ago2 and targets ADAR1 in IFN-stimulated cells. Overall, we propose that, by reducing ADAR1, IFN-I-induced miR-3614-5p contributes to lowering the activation threshold of innate sensors. Our findings provide new insights into the role of miR-3614-5p, placing it as a potential fine tuner of dsRNA metabolism, cell homeostasis and innate immunity.</p
Union Pacific (UP) 3614
No full textA photograph print showing Union Pacific (UP) 3614, 2-8-8-0, X-3614 [mixed freight extra - see white flags], eastbound, coming down Sherman Hill, near Otto, WY, 40 mph
Illinois Central (IC) 3614
No full textA photograph print showing the Illinois Central (IC) 3614, 2-10-0, Chicago, IL
Denver & Rio Grande Western (DRGW) 3614
No full textA photograph print showing Denver & Rio Grande Western (DRGW) 3614, 2-8-8-2 (Alco, class L-132), Minturn, CO
Block Card 3614 Berkeley Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3614 Berkeley Drive (Toledo, Ohio) | Cape Cod Style | Willys Park area (Toledo, Ohio) | Extension of Dixon Place (Toledo, Ohio
Block Card 3614 Glendale Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 3614 Glendale Avenue (Toledo, Ohio) | Dwelling | Albert J. Corey Company Beverly Downs (Toledo, Ohio) | South Toledo Area (Toledo, Ohio
Block Card 3614 Rugby Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Colonial Revival Style | 3614 Rugby Drive (Toledo, Ohio) | Dwelling | Beverly Place 1st Extension (Toledo, Ohio) | South Toledo Area (Toledo, Ohio
Block Card 3614 Torrance Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3614 Torrance Drive (Toledo, Ohio) | Cabin Style | Almeda Heights (Toledo, Ohio) | Willys Park area (Toledo, Ohio) | West Toledo (Toledo, Ohio
Block Card 3614 Greengate Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 3614 Greengate Drive (Toledo, Ohio) | Dwelling | Byrne Way Subdivision (Toledo, Ohio) | South Toledo Area (Toledo, Ohio
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