1,725,842 research outputs found
The long noncoding RNA loc107053557 acts as a gga-miR-3530-5p sponge to suppress the replication of vvIBDV through regulating STAT1 expression
No full textInfectious bursal disease virus (IBDV) causes immunosuppression and high mortality in young chickens. Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are important regulators during viral infection. However, detailed the regulatory mechanisms of lncRNA-miRNA-mRNA have not yet been described in IBDV infection. Here, we analysed the role of lncRNA53557/gga-miR-3530-5p/STAT1 axis in very virulent IBDV (vvIBDV) infection. Evidently upregulated expression of lncRNA53557 was observed in bursa of Fabricius and DT40 cells. Meanwhile, overexpression of lncRNA53557 promoted STAT1 expression and inhibited vvIBDV replication and vice versa, indicating that the upregulation of lncRNA53557 was part of the host antiviral defence. The subcellular fractionation assay confirmed that lncRNA53557 can be localized in the cytoplasm. Further, dual-luciferase reporter, RNA pulldown, FISH and RT-qPCR assays revealed that lncRNA53557 were directly bound to gga-miR-3530-5p and had a negative regulatory relationship between them. Subsequent mechanistic analysis showed that lncRNA53557 acted as a competing endogenous RNA (ceRNA) of gga-miR-3530-5p to relieve the repressive effect of gga-miR-3530-5p on its target STAT1, as well as Mx1, OASL, and ISG15, thereby suppressing vvIBDV replication. The study reveals that a network of enriched lncRNAs and lncRNA-associated ceRNA is involved in the regulation of IBDV infection, offering new insight into the mechanisms underlying IBDV-host interaction.</p
Block Card 3530 Helene Court
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch Style | 3530 Helene Court (Toledo, Ohio) | Dwelling | Wajer-Brooks Addition | Trilby Are
Block Card 3530 Terrace Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3530 Terrace Drive (Toledo, Ohio) | Ranch houses | Ratterrees Boulevard Terrace | Buckeye Basin | North Toledo (Toledo, Ohio
Block Card 3530 Burton Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3530 Burton Avenue (Toledo, Ohio) | Cape Cod Style | Almeda Heights Second Addition (Toledo, Ohio) | Willys Park area (Toledo, Ohio) | West Toledo (Toledo, Ohio
Block Card 3530 Dixie Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: dwelling | 3530 Dixie Drive (Toledo, Ohio) | Ranch houses | Ratterrees Boulevard Terrace | Buckeye Basin (Toledo, Ohio) | North Toledo (Toledo, Ohio
Union Pacific (UP) 3530
No full textA photograph print showing the Union Pacific (UP) 3530, 2-8-8-0, and 3812, 4-6-6-4, double-heading an eastbound mixed freight train along the Snake River near King Hill, ID, 60 cars, 40 mph, on the UP Main Line
Block Card 3530 Victory Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | Dwelling | Dell Haven II Subdivision (Toledo, Ohio) | Inverness Area (Toledo, Ohio) | 3530 Victory Avenue (Toledo, Ohio
Block Card 3530 Bluff Street
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Carpenter Gothic Style | 3530 Bluff Street (Toledo, Ohio) | Dwelling | Auburndale Amended Plat Extension (Toledo, Ohio) | Auburndale Area (Toledo, Ohio
Union Pacific (UP) 3530 & 3551
No full textA photograph print showing Union Pacific (UP) 3530, 2-8-8-0, on mixed freight train No. 655 with loco 3551, 2-8-8-0, pushing at rear, climbing up out of Burnt River Canyon near Pleasant Valley, OR, 64 cars, 20 mph. (See also 2008.008.2733
Block Card 3530 Greengate Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 3530 Greengate Drive (Toledo, Ohio) | Dwelling | Byrne Way Subdivision (Toledo, Ohio) | South Toledo Area (Toledo, Ohio
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