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Additional file 3 of Circular RNA circEMB promotes osteosarcoma progression and metastasis by sponging miR-3184-5p and regulating EGFR expression
No full textAdditional file 3: Fig. S2. The relationship between the miR-3184-5p and clinicopathological characteristics. (A) Comparison of miR-3184-5p expression between non-metastatic and metastatic OSA tissues. (B) Distribution characteristics of miR-3184-5p expression in the TNM stage of OSA. (C) In the 53-patients cohort, according to the median value of miR-3184-5p expression, they were divided into high expression group and low expression group. (D) Overall survival analysis of patients with low and high expression of miR-3184-5p using log-rank test and Kaplan–Meier analysis. (E) Correlation analysis between circEMB and miR-3184-5p in OSA tissues. (F) Relative miR-3184-5p levels in OSA cell lines and normal osteoblasts were ascertained by qRT-PCR. Data are expressed as mean ± SD (n = 3) (*, p < 0.05; **, p < 0.01; ***, p < 0.001)
Additional file 4 of Circular RNA circEMB promotes osteosarcoma progression and metastasis by sponging miR-3184-5p and regulating EGFR expression
No full textAdditional file 4: Fig. S3. Overexpression of miR-3184-5p was associated with decreased OSA cell migration and invasion, nor increased apoptosis and G1/S phase arrest. (A-B) The proliferation ability of OSA cells after overexpression of miR-3184-5p was determined by CCK-8. (C-F) Transwell assays detected the changes of invasion and metastasis ability of OSA cells with or without miR-3184-5p overexpression. (C and E) Refer to 143B, while (D and F) refer to U2OS. Scale bar, 100 μm. (G-H) Flow cytometric apoptosis analysis detected the changes of apoptosis of OSA cells with or without miR-3184-5p overexpression. (I-K) Flow cytometry assay showed the regulation of cell cycle by overexpressing miR-3184-5p. Data are expressed as mean ± SD (n = 3) (*, p < 0.05; **, p < 0.01; ***, p < 0.001)
Additional file 2 of Circular RNA circEMB promotes osteosarcoma progression and metastasis by sponging miR-3184-5p and regulating EGFR expression
No full textAdditional file 2: Fig. S1. Verification of transfection efficiency of miR-3184-5p in OSA cells. (A) After the transfection of mimic and inhibitor of miR-3184-5p, qRT-PCR was used to detect the transfection efficiencies. Data are expressed as mean ± SD (n = 3) (*, p < 0.05; **, p < 0.01; ***, p < 0.001)
Santa Fe (ATSF) 3184
No full textA photograph print showing the Santa Fe (ATSF) 3184, 2-8-2 (BLW class 3160), South Shawnee, OK. [see also 2008.008.1307, photo taken at same time
Astronomische Nachrichten nos 3166-3184
No full textR. R. Astronomische Nachrichten nos 3166-3184. In: Bulletin astronomique, tome 10, 1893. pp. 478-488
Santa Fe (ATSF) 3184
No full textA photograph print showing the Santa Fe (ATSF) 3184, 2-8-2 (BLW class 3160), South Shawnee, OK. [see also 2008.008.6257, photo taken at same time
Astronomische Nachrichten, nos 3184-3196
No full textR. Astronomische Nachrichten, nos 3184-3196. In: Bulletin astronomique, tome 10, 1893. pp. 529-536
Block Card 3184 Astor Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Ranch houses | 3184 Astor Avenue (Toledo, Ohio) | Dwelling | Replat of Vanderbilt Terrace (Toledo, Ohio) | South Toledo Area (Toledo, Ohio
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