1,726,451 research outputs found
HOXB1 is a direct target of miR-3175.
No full text<p><b>(A)</b> Diagram of the seed sequence of the miR-3175-binding site within the wild-type HOXB1 3′-UTR and the design of the mutated HOXB1 3′-UTR sequence. The mutated HOXB1 3′-UTR sequence is labeled in red. <b>(B)</b> Luciferase assay of U87 cells after cotransfection with the wild-type or mutant HOXB1 3′-UTR and miR-3175 mimics or miR-3175 mimics NC. The pRL-TK Renilla luciferase reporter vector was used as the internal control. Luciferase activity = firefly luciferase/Renilla luciferase. <b>(C)</b> HOXB1 mRNA expression is modulated by miR-3175. Glioma cell lines were transfected with miR-3175 mimics, miR-3175 inhibitor and the corresponding NCs, and incubated for 72 h. Cells were harvested and assayed using qRT-PCR. <b>(D-E)</b> Western blot analysis of HOXB1 protein levels in glioma cell lines after transfection with miR-3175 mimics, miR-3175 inhibitor and the corresponding NCs at 72 h. <b>(F)</b> The correlation between HOXB1 and miR-3175 expression in human glioma tissue specimens was analyzed with Spearman’s rank correlation. All results are representative of three independent experiments, and a statistical analysis is performed (mean ± SD, *<i>P</i> < 0.05, **<i>P</i> < 0.01, and ***<i>P</i> < 0.001).</p
Mandurama photograph collection [3175] [picture] /
No full textAlso available in an electronic version via the Internet at: http://nla.gov.au/nla.pic-an8526486-3175
Block Card 3175 Aldringham Road
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Cape Cod Style | Dwelling | 3175 Aldringham Road (Toledo, Ohio) | Extension of Forest View Addition (Toledo, Ohio) | Deveaux Area (Toledo, Ohio) | West Toledo (Toledo, Ohio
Block Card 3175 River Road
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Tudor Revival Style | attached garage | 3175 River Road (Toledo, Ohio) | dwelling | East Marengo Addition (Toledo, Ohio) | South Toledo (Toledo, Ohio
Aberrant expression of miR-3175 in glioma cell lines affects their proliferation, invasion, and apoptosis.
No full text<p><b>(A)</b> MiR-3175 expression levels in 40 human glioma tissue specimens and 8 non-neoplastic brain tissue specimens were assessed by qRT-PCR. <b>(B)</b> qRT-PCR analysis showed miR-3175 expression in glioma cell lines A172, U251, and U87. <b>(C)</b> Equal concentrations of miR-3175 mimics, mimics NC, miR-3175 inhibitor and inhibitor NC were transfected into U87 cells using Lipofectamine 2000. The expression levels of miR-3175 were evaluated by qRT-PCR at 24 h after transfection. <b>(D)</b> An MTT cell proliferation assay was performed at 48 h after transfection with equal concentrations of miR-3175 inhibitor and inhibitor NC in glioma cell lines. <b>(E-F)</b> Scratch wound assay of glioma cell lines transfected with equal concentrations of miR-3175 inhibitor and inhibitor NC. The wound gaps were photographed and measured at 0 and 24 h (40 ×, Scale bars = 200 μm). <b>(G-H)</b> Transwell assay of glioma cell lines transfected with equal concentrations of miR-3175 inhibitor and inhibitor NC. The migrated cells were photographed and counted in three non-overlapping, randomly selected fields (100 ×, Scale bars = 200 μm). <b>(I-J)</b> Flow cytometric analysis of apoptosis in glioma cell lines after transfection with equal concentrations of miR-3175 inhibitor and inhibitor NC. Apoptosis was assessed with Annexin V-FITC/PI. All results are representative of three independent experiments, and a statistical analysis is performed (mean ± SD, *<i>P</i> < 0.05, **<i>P</i> < 0.01, and ***<i>P</i> < 0.001).</p
Block Card 3175 Cherry Street
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Bungalow Style | Dwelling | 3175 Cherry Street (Toledo, Ohio) | Cottage Park Addition (Toledo, Ohio) | Lagrange-Central Area (Toledo, Ohio) | North Toledo (Toledo, Ohio
Faint satellite population of the NGC-3175 Group – a Local Group analogue
Get PDFIn this paper, we identify and study the properties of low-mass dwarf satellites of a nearby Local Group analogue – the NGC-3175 galaxy group with the goal of investigating the nature of the lowest mass galaxies and the ‘Missing Satellites’ problem. Deep imaging of nearby groups such as NGC-3175 is one of the only ways to probe these low-mass galaxies which are important for problems in cosmology, dark matter and galaxy formation. We discover 553 candidate dwarf galaxies in the group, the vast majority of which have never been studied before. We obtained R and B band imaging, with the European Southern Observatory 2.2 m, around the central ∼500 kpc region of NGC-3175, allowing us to detect galaxies down to ∼23 mag (MB ∼ −7.7 mag) in the B band. In the absence of spectroscopic information, dwarf members and likely background galaxies are separated using colour, morphology, and surface brightness criteria. We compare the observed size, surface brightness, and mass scaling relations to literature data. The luminosity function with a faint end slope of α = −1.31, is steeper than that observed in the Local Group. In comparison with simulations, we find that our observations are between a pure Λ cold dark matter model and one involving baryonic effects, removing the apparent problem of finding too few satellites as seen around the Milky Way
HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma.
No full textThe HOXB1 gene plays a critical role as an oncogene in diverse tumors. However, the functional role of HOXB1 and the mechanism regulating HOXB1 expression in glioma are not fully understood. A preliminary bioinformatics analysis showed that HOXB1 is ectopically expressed in glioma, and that HOXB1 is a possible target of miR-3175. In this study, we investigated the function of HOXB1 and the relationship between HOXB1 and miR-3175 in glioma. We show that HOXB1 expression is significantly downregulated in glioma tissues and cell lines, and that its expression may be closely associated with the degree of malignancy. Reduced HOXB1 expression promoted the proliferation and invasion of glioma cells, and inhibited their apoptosis in vitro, and the downregulation of HOXB1 was also associated with worse survival in glioma patients. More importantly, HOXB1 was shown experimentally to be a direct target of miR-3175 in this study. The downregulated expression of miR-3175 inhibited cell proliferation and invasion, and promoted apoptosis in glioma. The oncogenicity induced by low HOXB1 expression was prevented by an miR-3175 inhibitor in glioma cells. Our results suggest that HOXB1 functions as a tumor suppressor, regulated by miR-3175 in glioma. These results clarify the pathogenesis of glioma and offer a potential target for its treatment
- …
