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Serum microRNA-122 predicts survival in patients with liver cirrhosis
No full textBackground: Liver cirrhosis is associated with high morbidity and mortality. MicroRNAs (miRs) circulating in the blood are an emerging new class of biomarkers. In particular, the serum level of the liver-specific miR-122 might be a clinically useful new parameter in patients with acute or chronic liver disease.
Aim: Here we investigated if the serum level of miR-122 might be a prognostic parameter in patients with liver cirrhosis.
Methods: 107 patients with liver cirrhosis in the test cohort and 143 patients in the validation cohort were prospectively enrolled into the present study. RNA was extracted from the sera obtained at the time of study enrollment and the level of miR-122 was assessed. Serum miR-122 levels were assessed by quantitative reverse-transcription PCR (RT-PCR) and were compared to overall survival time and to different complications of liver cirrhosis.
Results: Serum miR-122 levels were reduced in patients with hepatic decompensation in comparison to patients with compensated liver disease. Patients with ascites, spontaneous bacterial peritonitis and hepatorenal syndrome had significantly lower miR-122 levels than patients without these complications. Multivariate Cox regression analysis revealed that the miR-122 serum levels were associated with survival independently from the MELD score, sex and age.
Conclusions: Serum miR-122 is a new independent marker for prediction of survival of patients with liver cirrhosis
Regulation of miR-122 targets by chemically protected antagomir-122/miR-122-duplexes
No full text<p><b>Copyright information:</b></p><p>Taken from "Specificity, duplex degradation and subcellular localization of antagomirs"</p><p></p><p>Nucleic Acids Research 2007;35(9):2885-2892.</p><p>Published online 16 Apr 2007</p><p>PMCID:PMC1888827.</p><p>© 2007 The Author(s)</p> () Schematic description of the two different duplexes used. () Steady-state mRNA levels of miR-122 target genes in livers of mice treated with the indicated modified antagomir-122/miR-122-duplexes. Expression was measured using RT-PCR. Fold-regulation indicates the ratio of expression levels of the means of mice treated with antagomir-122/miR-122 duplex compared to the PBS group. The upper row shows a northern blot of liver RNA for miR-122. As controls, duplexes were added to 5 μg total kidney RNA and loaded on polyacrylamide gels before (‘input’) or after the Trizol protocol (‘Trizol’). Each lane represents an individual animal. Gapdh was used as a loading control, Gapdh-RT denotes a control without reverse transcription.
Dose- and time-dependency of miR-122 target regulation by antagomir-122
No full text<p><b>Copyright information:</b></p><p>Taken from "Specificity, duplex degradation and subcellular localization of antagomirs"</p><p></p><p>Nucleic Acids Research 2007;35(9):2885-2892.</p><p>Published online 16 Apr 2007</p><p>PMCID:PMC1888827.</p><p>© 2007 The Author(s)</p> Steady-state mRNA levels of miR-122 target genes in livers of mice treated with the indicated amounts of antagomir-122. Expression was measured by RT-PCR. Each lane indicates an individual animal. The glyceraldehyde-3-phosphate dehydrogenase gene (Gapdh) was used as a loading control. Gapdh-RT denotes a control without reverse transcription. Gene symbols are shown in accordance with the International Standardized Nomenclature (). The upper row shows a northern blot of liver RNA for miR-122. Each lane represents an individual animal. () Dose-dependency. () Time-course
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