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Results of molecular polymorphism analysis of Toxoplasma gondii isolates from free-living animal populations in Poland
No full textThe dataset contains data obtained during the Miniatura research project (8th edition). The main goal of the Project was to optimize methods for the detection and genotyping of Toxoplasma gondii and to perform a preliminary assessment of the prevalence and genetic polymorphism of this parasite in populations of free-living animals in Poland. The study included biological samples collected from 230 individuals representing five species of free-living animals (beaver, hare, deer, wild boar, raccoon dog). The dataset provides complete documentation of successive stages of the project, including DNA extraction and assessment of DNA quantity and quality, detection of T. gondii using the B1 marker (nested PCR), genotyping of isolates using the MLST approach (12 markers), analysis of microsatellite markers (15 markers), as well as results and raw files of bioinformatic analyses conducted for the obtained sequences. The data include results of PCR amplification, electrophoretic separation, sequencing, analyses of nucleotide and amino acid polymorphisms, similarity matrices, phylogenetic analyses, genetic distance calculations, and in silico restriction digestion. Each sample was assigned a unique identification code, ensuring full traceability of the material from DNA extraction through bioinformatic analyses to sequence deposition in the GenBank database. A central metadata registry contains information on sample origin, species, tissue type, sample preparation method, and links between data generated at individual stages of the project. The hierarchical folder structure of the repository reflects the workflow of the study. The dataset constitutes a comprehensive resource for the analysis of genetic diversity of T. gondii in free-living animal populations, enables comparisons with reference data deposited in public databases, and may be used in studies on toxoplasmosis epidemiology
Prezentacja Inauguracyjna Roku Akademickiego 2025/2026
No full text9 października 2025 roku w Centrum Kongresowym Uniwersytetu Przyrodniczego w Lublinie odbyła się uroczysta – w roku obchodów Jubileuszu 70-lecia – Inauguracja Roku Akademickiego 2025/2026. W wydarzeniu licznie uczestniczyli przedstawiciele władz Uczelni, kadra akademicka, zaproszeni goście oraz nowo przyjęci studenci i doktoranci – łącznie blisko 500 osób. Uroczystości przewodniczył JM Rektor, prof. dr hab. Krzysztof Kowalczyk. Z okazji inauguracji przygotowana została okolicznościowa prezentacja nawiązująca do 70-lecia Uniwersytetu, podkreślająca dorobek, rozwój i znaczenie uczelni w środowisku akademickim
Composition, physicochemical, fertilizing and ecotoxicological properties of sewage sludges and their contaminant content
No full textThese are raw research data concerning the elementary composition, physicochemical, fertilizing and ecotoxicological properties of sewage sludges and their inorganic and organic contaminant content (heavy metals and 16 polycyclic aromatic hydrocarbons)
Distribution of contaminants between solid digestate, liquid fraction and biogas after sewage sludge anaerobic digestion
No full textSewage sludge contains contaminants of different types and is characterized by a large number of pathogenic vectors. As a result, it must undergo treatment or stabilization (e.g., anaerobic digestion (AD)) before environmental disposal. AD-derived products (solid digestate and liquid fraction) can be used as fertilizers. During this process, biogas is produced, and used for energy purposes. All these components can be contaminated with various compounds, whose amount depends on the feedstocks used in the process (and their mutual proportions). These are research data concerning the distribution of contaminants between the individual phases (solid digestate, liquid fraction, and biogas) during sewage sludge AD
Honey bee physiology in the context of the pollen monodiet as a key environmental stressor
No full textThe aim of studies is to determine the influence of monodiet as a natural stress factor on the activities of the proteolytic and antioxidant systems, as well as biochemical markers and the concentration of compounds responsible for energy reserves in the hemolymph of workers along with their aging processes. The monodiet will be considered in the context of the following pollen: rape, phacelia, buckwheat and goldenrod.1-day-old workers will be placed in cages. The cages will be divided into five groups. The control group will be fed sugar dough, the research groups will receive sugar dough with 8% addition of the appropriate pollen. Hemolymph will be collected from workers in each group every 7 days. The activities of acidic, neutral and alkaline proteases and their inhibitors, superoxide dismutase (SOD), peroxidase (GPx), catalase (CAT), glutathione S-transferase (GST) and total antioxidant capacity (TAC) will be determined in the hemolymph. In addition, the activities of biochemical markers, i.e. aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT) and the concentrations of compounds responsible for maintaining energy reserves, i.e. glucose, glycogen and triglycerides will be determined.The results of this study will enrich our understanding of how monodiet affects the action of key compounds involved in the body's immune / response. Determining whether and how a single pollen affects the bee's response is part of the latest research in the field of nutriphysiology, ecology of pollen plants and shaping the landscape through specific plantings. Determining the impact of individual mono-diets on the immune processes of bees will be one of the first steps in developing a balanced diet for these insects. The end result of the research, in addition to scientific publications, will be the selection of pollen with the most beneficial effect on the functioning of the organism of bees
Changes in the total content and bioavailability of heavy metals during sewage sludge composting and anaerobic digestion
No full textComposting and anaerobic digestion are typical biological process used for sewage sludge organic matter stabilization. Both processes lead to the loss of labile organic matter and as a result of it to the transformation of metals into more stable fractions, which results in their decreased bioavailability and mobility. Unlike organic contaminants that biodegrade as a result of these processes, metals are persistent and become concentrated due to the loss of water and labile organic matter. However, during composting leaching of soluble heavy metals were also observed. These are research data concerning the systematic comparison of composting and anaerobic digestion of sewage sludges in terms of changes in the total content and forms of heavy metals (Cd, Cu, Ni, Pb, Zn and Cr). The primary goal of the research was to understand the fate of heavy metals during composting and anaerobic digestion, with particular emphasis on changes in bioavailability of heavy metals
Temporal and spatial variability of physico-chemical properties in reclaimed soil after borehole mining
No full textMining contributes to the creation of degraded areas that are difficult to rehabilitate because they are subject to various transformations. Specifically, sulphur extraction using the Frasch borehole method causes multidirectional changes of soil properties and thus presents challenges for post-mining rehabilitation. The aim of the current study was to evaluate the effectiveness of agricultural reclamation and to understand how the soil recovers over time by assessing the spatio-temporal variability of the reclaimed soil after borehole sulphur mining. Selected soil properties and spectral indices based on satellite data were analysed: before reclamation, three years after reclamation and 15 years after reclamation. Texture, bulk density, porosity, water capacity, TOC and pH were measured and distribution maps of these parameters were generated using kriging. Additionally, maps of spectral indices (Green Normalized Difference Vegetation Index,Normalized Difference Soil Moisture Index, Soil Adjusted Vegetation Index, EnhancedVegetation Index and Red–Green Index) based on Landsat imagery were created tosupport the field survey data. The soil revealed high temporal and spatial variability, asevidenced by the distributions of measured parameters and their coefficients of variationat the reclamation stages. Multidirectional remodelling of the soil structure occurred,resulting in changes in the differential porosity, field water capacity, texture, reactionand TOC. Statistically confirmed differences both in soil parameters between samplingpoints and in field means between reclamation stages were observed. The variability ofthe physico-chemical parameters at the individual sampling point also differed betweenthe reclamation stages. We observed a general trend towards homogenisation of thespatial distributions of the soil parameters. This was also confirmed by the maps ofspectral indices, which showed establishment and development of a continuousvegetation cover and the subsequent improvement of its condition.Mining contributes to the creation of degraded areas that are difficult to rehabilitate because they are subject to various transformations. Specifically, sulphur extraction using the Frasch borehole method causes multidirectional changes of soil properties and thus presents challenges for post-mining rehabilitation. The aim of the current study was to evaluate the effectiveness of agricultural reclamation and to understand how the soil recovers over time by assessing the spatio-temporal variability of the reclaimed soil after borehole sulphur mining. Selected soil properties and spectral indices based on satellite data were analysed: before reclamation, three years after reclamation and 15 years after reclamation. Texture, bulk density, porosity, water capacity, TOC and pH were measured and distribution maps of these parameters were generated using kriging. Additionally, maps of spectral indices (Green Normalized Difference Vegetation Index,Normalized Difference Soil Moisture Index, Soil Adjusted Vegetation Index, EnhancedVegetation Index and Red–Green Index) based on Landsat imagery were created tosupport the field survey data. The soil revealed high temporal and spatial variability, asevidenced by the distributions of measured parameters and their coefficients of variationat the reclamation stages. Multidirectional remodelling of the soil structure occurred,resulting in changes in the differential porosity, field water capacity, texture, reactionand TOC. Statistically confirmed differences both in soil parameters between samplingpoints and in field means between reclamation stages were observed. The variability ofthe physico-chemical parameters at the individual sampling point also differed betweenthe reclamation stages. We observed a general trend towards homogenisation of thespatial distributions of the soil parameters. This was also confirmed by the maps ofspectral indices, which showed establishment and development of a continuousvegetation cover and the subsequent improvement of its condition
Occurrence and dissipation mechanisms of organic contaminants during sewage sludge anaerobic digestion: A critical review
No full textImmunological and oxidoreduction indicators in biological material (yolk blood) taken from turkeys receiving antibiotics
No full textThe research hypothesis postulates that early antibiotic administration inhibits yolk sac resorption and decreases the transfer of maternal antibodies to the circulatory system of poults, which may reduce their humoral immunity during the first few days post hatch, thus compromising immune function (immunosuppression) and the antioxidant status of birds. Diets containing coccidiostats, which are also classified as antibiotics, can exert similar effects in turkeys. Four experiments will be performed on young female Hybrid Converter turkeys to analyze their immune and redox responses to the experimental factors. The aim of Experiment 1 will be to determine whether early antibiotic administration or feeding diets containing a coccidiostat affect the rate of yolk sac resorption and maternal antibody transfer as well as immune function (in particular specific immune responses, including vaccine-induced responses) and the antioxidant status of growing turkeys. The aim of Experiment 2 will be to determine whether early antibiotic administration combined with feeding diets containing a coccidiostat affect the rate of yolk sac resorption and maternal antibody transfer as well as immune function and the antioxidant status of growing turkeys. The aim of Experiment 3 will be to determine whether early antibiotic administration affects the effective activation of the immune and antioxidant systems in turkeys exposed to infections in early or later stages of rearing. The aim of Experiment 4 will be to determine the effects of early antibiotic administration in turkeys fed diets containing a coccidiostat (additive effect), exposed to infections in early or later stages of rearing. The study will involve an evaluation of the effects exerted by the experimental factors on: the rate of yolk sac resorption and maternal antibody transfer from the yolk sac to poults, immune responses, i.e. non-specific cellular immunity, specific humoral and adaptive immunity, redox reactions in the immune system, the expression of immune-related genes and genes involved in the control of redox homeostasis, the expression of genes and the values of indicators related to gut integrity, and the histology of lymphoid organs. The body weight gains and mortality rates of turkeys will also be determined
Immunological, oxidoreductive, biochemical indicators, microbial enzymes in biological material taken from rats fed a high-fat diet with the addition of chromium
No full textObesity is one of the most prevalent diseases of civilization in the 21st century. It can be influenced by genetic, epigenetic, socio-economic, biological, and behavioural factors. However, a high-fat diet seems to be of particular importance in the pathophysiology of obesity. Chromium (III) (Cr) is a key microelement involved in the metabolism of carbohydrates, proteins, and fats in humans and animals. Due to these properties of Cr, it is popularly used as a factor supporting the treatment of type 2 diabetes and as a component of supplements used in slimming treatments. Currently, the most popular form of Cr used in dietary supplements is picolinate (Cr-Pic), an organic compound of trivalent Cr. The use of Cr-Pic has many benefits, including weight reduction and increased muscle mass. However, due to the relatively low bioavailability of chromium picolinate, other forms of this element are sought that could be better utilized by the body. For this reason, researchers are increasingly interested in complexes of Cr with amino acids as well as inorganic chromium nanoparticles. Recently, nanoparticles have emerged as important players in modern science, including nutritional status of the host in health and disease. But, in the recent own experiment on young growing rats fed high-fat diet supplemented with different forms of chromium (Cr (III) picolinate, Cr (III) methionine, Cr nanoparticles) some important worrying results have been obtained. It has been shown that one should be careful when using chromium supplementation to counteract obesity, because it may be associated with the risk of deterioration of the functioning of some internal organs. In the present project the experimental schema is more adapted to common dietary and physiological environment that an obese consumer face. The laboratory growing rats will be first subjected to high-fat diet in order to induce undesired changes in the body functions (initial period of the experiment); then the obese animals will be divided into several groups and subjected to dietary treatments imitating different dietary behaviours and decisions of obese consumers (stick – or not, to an “old” dietary high-fat patterns with or without chromium supplementation support). Two forms of supplemental Cr will be used, namely most popular chromium picolinate (Cr-Pic) and novel chromium nanoparticles (Cr-NP). In the project we postulate that negative intestinal, vascular and hepatic effects associated with chronic consumption of high-fat diet could be subsequently alleviated through dietary supplementation of various forms of chromium and/or switching to low-fat diet. We hypothesized that the switch away from high-fat dietary habits combined with chromium supplementation (picolinate or nanoparticles) would beneficially affect physiological responses in the gastrointestinal tract and thus positively modulate the health status of the body. Moreover, the effects observed in the treatments with Cr-Pic will be more pronounced upon Cr-NP due to higher absorbability and reactivity of nanoparticles. The rat Wistar (Cmdb:Wi CMDB) will be used as a research model, i.e. an established host model for nutritional/metabolic studies, including the intestinal, immunological, vascular and hepatic responses to nutritional interventions. The main intention of the project’s authors is to bring as much as possible new insight with regard to the question whether negative intestinal, vascular and hepatic effects associated with chronic consumption of high-fat diet could be subsequently alleviated through dietary supplementation of various forms of chromium and/or switching to low-fat diet. Taking into account an urgent need to establish true action of dietary nanoparticles in the gastrointestinal tract and the whole body, a paramount attention will be paid to Cr-NP and their action will be compared to commonly used dietary supplement Cr-Pic. The negative or positive impact of supplemental Cr on the microbiota living in the lower gut (with novel approach assessing microbial intra- and extracellular enzymatic activities), thus affecting local (in the upper and lower gastrointestinal tract), hepatic and systemic (metabolic, i.e. blood haematology and biochemistry parameters) health status of the obese host with different eating habits will be assessed. Jerzy Juskiewicz, Instytut Rozrodu Zwierzat i Badan Zywnosci Polskiej Akademii Nauk 497818 ZSUN