Kagawa Prefectural University of Health Sciences Research Result Repository
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Clarifying the Role and Function of Community-Based Programs in Frailty Prevention
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Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellulart trap phagocytosis
Get PDF香川県立保健医療大学博士(臨床検査学)Presepsin (P-SEP) is a specific biomarker for sepsis. Monocytes produce P-SEP by phagocytosing neutrophil extracellular traps (NETs). Herein, we investigated whether M1 macrophages (M1 MΦs) are the primary producers of P-SEP after NET phagocytosis. We co-cultured M1 MΦs and NETs from healthy participants, measured P-SEP levels in the culture medium supernatant, and detected P-SEP using western blotting. When NETs were co-cultured with M1 MΦs, the P-SEP level of the culture supernatant was high. Notably, we demonstrated, for the first time, the intracellular kinetics of P-SEP production by M1 MΦs via NET phagocytosis: M1 MΦs produced P-SEP intracellularly 15 min after NET phagocytosis and then released it extracellularly. In a sepsis mouse model, the blood NET ratio and P-SEP levels, detected using ELISA, were significantly increased (p < 0.0001). Intracellular P-SEP analysis via flow cytometry demonstrated that lung, liver, and kidney MΦs produced large amounts of P-SEP. Therefore, we identified these organs as the origin of M1 MΦs that produce P-SEP during sepsis. Our data indicate that the P-SEP level reflects the trend of NETs, suggesting that monitoring P-SEP can be used to both assess NET-induced organ damage in the lungs, liver, and kidneys during sepsis and determine treatment efficacy
End-of-life care provided by visiting nurses for family caregivers living apart from their care recipients living in rural areas of Japan
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Assessment of the nursing intervention program which softens semantic attachment with negative experience by rumination - randomized controlled trial for depression. -
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Overexpression of the PPAR‑γ protein in primary Ta/T1 non‑muscle‑invasive urothelial carcinoma
Get PDF香川県立保健医療大学博士（臨床検査学）application/pdfPeroxisome proliferator‑activated receptor‑γ(PPAR‑γ) is a well‑known nuclear receptor that is activated in the nucleus to regulate several transcription factors. Its expression patterns have been examined in various types of cancer. The present study investigated the expression patterns of PPAR‑γ in non‑muscle‑invasive urothelial carcinoma. The expression rates of PPAR‑γ, p53 and Ki‑67 were compared to determine whether PPAR‑γ may be considered as an immunobiomarker for bladder cancer. The intensity and extent of PPAR‑γ expression were evaluated in 79 cases of non‑muscle‑invasive urothelial carcinoma (30 cases of papillary carcinoma low‑grade, 30 cases of high‑grade and 19 cases of carcinoma in situ) and 30 non‑malignant cases. The nuclear overexpression of PPAR‑γ was frequently observed in non‑muscle‑invasive urothelial carcinoma (63/79 cases) but was rarely detected in non‑malignant cases (2/30 cases). The histological proliferation types of non‑muscle‑invasive urothelial carcinoma revealed that PPAR‑γ was more frequently overexpressed in papillary carcinoma (54/60 cases) than in carcinoma in situ (9/19 cases). Immunohistochemical staining demonstrated that PPAR‑γ was more useful as an immunobiomarker than p53 or Ki‑67 (diagnostic odds ratios; 55.13, 16.82 and 11.13, respectively). In summary, this study demonstrated that the expression patterns of PPAR‑γ were associated with histological proliferation type and that PPAR‑γ was expressed in the nuclei of papillary carcinoma cells. These findings suggested that immunohistochemical staining for PPAR‑γ may be used to comprehensively detect non‑muscle‑invasive urothelial carcinoma
Noninvasive embryo evaluation method combining timelapse images with biomarkers in follicular fluid and serum
Get PDF香川県立保健医療大学博士（臨床検査学）application/pdfThesis or DissertationTo increase the success rate of assisted reproductive technologies, embryos with high developmental and implantation potential should be identified and selected. Here, we sought to establish a noninvasive and highly accurate embryo selection method by combining time-lapse monitoring parameters（ at S2 and S3） with the following biomarkers in the follicular fluid（ FF） and serum samples obtained from study subjects（ using 70 eggs, FF, and serum in 58 cycles of conventional in vitro fertilization [c-IVF]）: dehydroepiandrosterone sulfate（ DHEA-S）, insulin-like growth factor 1 （IGF- 1）, reactive oxygen metabolites （d-ROMs）, biological antioxidant potential （BAP）, and an oxidative stress index （OSI: d-ROMs/BAP × 100）. Our analyses revealed significant positive correlations between some of the biomarkers in FF and serum: DHEA-S（ r=0.88; p<0.01）, IGF-1（ r=0.83; p<0.01）, d-ROMs（ r=0.55; p<0.01）, and OSI （r= 0. 45; p< 0. 01）. The BAP value was higher in FF than in serum in many cases. The follicular OSI in the abnormal fertilization（ AF） group tended to be higher than that in the normalfertilization（NF） group. The FF values of DHEA-S and IGF-1 and the serum DHEA-S tended to be higher in the NF group and blastocyst formation （BF） group. S 2 and S 3 were shortened in the NF and BF groups. These findings suggest that （1） oxidative stress is related to abnormal fertilization, （2） DHEA-S and IGF- 1 are associated with normal fertilization and blastocyst formation, and（ 3） S2 and S3 are useful timepoints for evaluating embryos
Activation and Expression of Peroxisome Proliferator-Activated Receptor Alpha Are Associated with Tumorigenesis in Colorectal Carcinoma
Get PDF香川県立保健医療大学博士（臨床検査学）application/pdfThesis or DissertationPeroxisome proliferator-activated receptor alpha (PPAR-????) belongs to the PPAR family and plays a critical role in inhibiting cell proliferation and tumorigenesis in various tumors.However, the role of PPAR-???? in colorectal tumorigenesis is unclear. In the present study, we found that fenofibrate, a PPAR-???? agonist, significantly inhibited cell proliferation and induced apoptosis in colorectal
carcinoma cells. In addition, PPAR-???? was expressed in the nucleus of colorectal carcinoma cells, and the expression of nuclear PPAR-???? increased in colorectal carcinoma tissue compared with that of normal epitheliumtissue (P<0.01).Thecorrelation between the expression of nuclear PPAR-???? and clinicopathological factors was evaluated in human colorectal carcinoma tissues, and the
nuclear expression of PPAR-???? was significantly higher in well-to-moderately differentiated adenocarcinoma than in mucinous adenocarcinoma (P<0.05). These findings indicate that activation of PPAR-???? may be involved in anticancer effects in colorectal carcinomas, and nuclear expression of PPAR-???? may be a therapeutic target for colorectal adenocarcinoma treatment
Comprehensive assessment of oxidative stress degrees and anti-oxidant potential in dialysis patients
Get PDF香川県立保健医療大学博士（臨床検査学）application/pdfThesis or DissertationWhen the kidney is compromised, oxidative stress is aggravated by an increased rate of
reactive oxygen species generation and a decreased rate of substance elimination. In addition, dialysis
treatment itself is reported to be associated with oxidative stress in patients with terminal renal
insufficiency. This study was conducted to determine the optimum dialysis treatment. Diacronreactive
oxygen metabolite and biological anti-oxidant potential tests were performed to assess
oxidative stress levels. Increased oxidative stress and decreased anti-oxidant potential were found
immediately after dialysis. A comparison of dialysis membranes revealed that ABH®-PA
membranes exhibited a significantly lower rate of decrease in anti-oxidant potential in terms of
oxidative stress compared with Maxiflux® membranes. Analysis of biochemical parameters identified
significant strong positive correlations between diacron-reactive oxygen metabolites test
results and serum total protein, serum albumin, total cholesterol, high-density lipoprotein-cholesterol,
and low-density lipoprotein-cholesterol for the percentage decrease and between biological
anti-oxidant potential test results and inorganic phosphorus for pre-dialysis measurements.
ABH®-PA should be used as the dialysis membrane to minimize the increase in the degree of
oxidative stress caused by dialysis treatment. We recommend measuring serum total protein, serum
albumin, total cholesterol, and inorganic phosphorus before and after dialysis sessions to better
understand oxidative stress degrees and anti-oxidant potential
