19354 research outputs found
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The Harm Reduction Program
Full text linkPamphlet containing information on the Harm Reduction Program provided through the Buffalo Columbus Hospital.https://digitalcommons.buffalostate.edu/hivaidsedu/1006/thumbnail.jp
Flyer for Safety Awareness & Assault Prevention for the Lesbian, Gay, & Bisexual Community
Full text linkFlyer for a self defence class for the LGBTQ community.https://digitalcommons.buffalostate.edu/mdlevents/1004/thumbnail.jp
Facts You Should Know
Full text linkPamphlet containing information on HIV and where to receive help.https://digitalcommons.buffalostate.edu/hivaidsedu/1005/thumbnail.jp
Pamphlet for Benedict House
Full text linkPamphlet containing information on Benedict House.https://digitalcommons.buffalostate.edu/hivaidsedu/1010/thumbnail.jp
Safe Sex a How-To Guide
Full text linkHandout in English and Spanish containing information on safe sex practices, including: condom instruction, local STI statistics, and information on HIV/AIDS.https://digitalcommons.buffalostate.edu/hivaidsedu/1007/thumbnail.jp
Flyer for the Annual Buffalo Pride Parade & Rally
Full text linkFlyer for the Annual Buffalo Pride Parade & Rally.https://digitalcommons.buffalostate.edu/mdlevents/1018/thumbnail.jp
AIDS Service Providers Consortium of Western New York Pamphlet
Full text linkPamphlet containing information about the Aids Service Providers Consortium of Western New York.https://digitalcommons.buffalostate.edu/hivaidsedu/1002/thumbnail.jp
Flyer for the Cuckoo\u27s Nest
Full text linkAdvertisement for the Cuckoo\u27s Nest, featuring nightly drink specials.https://digitalcommons.buffalostate.edu/mdlevents/1005/thumbnail.jp
All Women Dance
Full text linkFlyer for an All Women\u27s Dance.https://digitalcommons.buffalostate.edu/mdlevents/1006/thumbnail.jp
Investigating the Inhibitory Effects of Coffee on DNA Amplification in Forensic Applications
No full textPolymerase chain reaction (PCR) is a cornerstone technique in forensic DNA analysis, enabling the amplification of minute quantities of genetic material. The presence of PCR inhibitors can significantly compromise amplification efficiency and accuracy. This study investigates the inhibitory effects of coffee – a commonly encountered contaminant at crime scenes – on the PCR amplification of human DNA. Various concentrations of coffee were introduced into PCR reactions to evaluate their impact on DNA yield, purity, and amplification success. Quantitative and qualitative analyses were performed using spectrophotometry, agarose gel electrophoresis, capillary electrophoresis, and quantitative PCR (qPCR). The results indicate a concentration-dependent inhibition of PCR, with significant amplification failure observed at higher concentrations of coffee. Polyphenolic compounds such as chlorogenic acid and tannins were suspected as key contributors to PCR inhibition, likely through direct interaction with DNA polymerase or by binding to DNA, thereby reducing its accessibility. These findings highlight the importance of recognizing environmental inhibitors such as polyphenols in forensic casework and stress the need for effective DNA purification strategies to ensure accurate amplification