727 research outputs found
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4D Microprinting of Dual-Shape and Triple-Shape Memory Polymers [data]
No full text4D microprinting of shape memory polymers (SMPs) offers high structural design freedom alongside the smart characteristics of SMPs. However, functionality has so far been limited to dual-shape memory behavior, while a triple-shape memory effect – allowing access to multiple temporary shapes – remains unexplored for 4D microprinted SMPs. Here, we present an approach based on photocrosslinkable semicrystalline polymers offering access to two ink formulations for the fabrication of dual- and triple-shape memory microstructures via multiphoton laser printing. Printing parameters are optimized for generation of complex 3D microarchitectures with high printing precision and shape fidelity and thermal characterization establishes protocols for assessing the shape memory effects. All structures show remarkable shape memory performance featuring stable shape fixation and repeatable full recovery to the original structure. Notably, triple-SMP structures store an additional temporary shape enabling sequential two-directional actuation holding great potential for the next generation of soft microrobots and adaptive microsystems
Minimal Couple Intervention to Improve Psychobiological Stress Resilience [Research Data]
No full textThe data are associated with the manuscript "Minimal Couple Intervention to Improve Psychobiological Stress Resilience". They were used to test the effects of a minimal couple intervention in everyday life
Climate policy portfolios that accelerate emission reductions [Data]
No full textThe corpus of national climate policies continues to grow - but to what effect? Using data on 3,917 policy instruments across 43 OECD countries and major emerging economies from 2000-2022, we show that national climate policy portfolios specializing in instrument types and sectors are associated with faster reductions in fossil CO2 emission intensity. Supported by exemplar country case studies, we also provide quantitative evidence that the effectiveness of climate policy is amplified by long-term emission reduction targets and the presence of dedicated governmental bodies including ministries and intergovernmental organisations. The cumulative effect of all climate policy portfolios over our study period amounts to 3.1 GtCO2 fewer emissions in 2022 relative to a no-policy counterfactual - substantially less than what’s needed to stay on track for the Paris Agreement goals
Stop frowning, it´s true: Reduced corrugator activity indicates increased positive affect after judging information as true [Dataset]
No full textIn line with the feelings-as-information theory, a body of research demonstrates more
positive (negative) judgments in positive (negative) affective states. Similarly, it has
been shown that people who experience positive (negative) affect also tend to judge
incoming information as more likely being true (false). Following the argumentation of
affect-congruent judgments, we assume that judging information as being true itself
possesses a positive affective component. In a truth effect study, we implemented two
judgment phases (10 minutes and 1 week after first exposure) in which 75 participants
judged the truth of in total 120 (new and repeated) statements. Addressing the present
research question, we assessed spontaneous facial reactions via electromyography after participants provided their truth judgments in each trial. Results reveal corrugator
relaxations after judging information as true (vs. false), indicating increased positive affect. Importantly, this finding was unaffected by the repetition status and subjective confidence regarding judgments
Sepsis alters NK cell transcriptional programs for stress, actin remodeling, and intracellular trafficking [data]
No full textBackground: Natural killer (NK) cells exert cytotoxicity against transformed and infected cells. In human sepsis, a suppressive NK cell receptor signature and defective effector molecule expression have been described. However, the transcriptional mechanisms underlying this phenotype remain poorly defined.
Methods: We analyzed microarray-based transcriptomic profiles of isolated peripheral NK cells from patients with sepsis, systemic inflammatory response syndrome (SIRS), and presurgical controls. Enrichment analyses of canonical pathways, biological processes, and cellular compartments were performed. Differential gene expression was validated in an independent cohort using a multiplex branched-DNA assay. Functional STAT phosphorylation responses ex vivo and proliferation marker expression were assessed by flow cytometry in independent patient samples.
Results: NK cells from sepsis patients displayed transcriptional signatures indicative of DNA replication stress, ER stress, altered cytoskeletal dynamics and vesicle trafficking. Despite enrichment of proliferation-associated transcriptional programs, NK cells showed no increase in Ki-67 expression, indicating impaired proliferative activity. In contrast, NK cells from SIRS patients exhibited downregulation of immune signaling pathways.
Conclusion: This study identifies early stress-associated transcriptional programs and impaired subcellular organization in circulating NK cells during sepsis. Dysregulated DNA replication and ER stress responses, along with altered vesicle trafficking linked to impaired small GTPase signaling, may contribute to NK cell dysfunction in sepsis and may inform the development of NK cell-based immunotherapeutic strategies in critical illness
Transcriptome analysis of medaka embryonic eye and ocular organoids [data]
No full textThe dataset has been generated in order to assess the temporal dynamics of lens-specific gene expression in ocular organoids and embryonic eye. Bulk RNA sequencing of total RNA from medaka fish (O. latipes) embryonic eye and medaka fish – derived ocular organoids at day 0, day 1, day 2, day 3 and day 4 of development. For total RNA sequencing we extracted RNA from whole organoids (composed of both lens and retina), the anterior part of day 1 and 2 embryos (dissected right behind optic vesicles or optic cups), and dissected eyes of 3 dpf and 4 dpf embryos. Samples were prepared in three replicates (indicated as samples S1, S2, and S3). Considering 3 h delay in organoid development with the respect to embryonic development (Zilova et al., 2021), organoids were collected for RNA isolation 3 h later than the embryonic tissue. Total RNA was isolated using Direct-zol RNA Microprep (Zymoresearch, Cat#:R2060) according to the manufacture protocol. Library preparation was performed with the NEBNext Ultra II RNA Library Prep Kit for Illumina with NEBNext Poly A Selection Kit and NEBNext Multiplex Oligos with UMI. Input RNA was quantified with the Qubit BR RNA assay, and qualified on the Agilent Tapestation (RNA Assay), libraries were quantified with the Qubit DNA HS Assay, and qualified on the Agilent Tapestion (D1000 Assay). Libraries were sequenced on the Illumina NextSeq 550 Sequencer. Library preparation and sequencing was performed by CCTP Deep Sequencing Facility of Heidelberg University. Samples are named as embryo_d0_S1 (refer to embryo sample from day 0, replicate sample 1) or lens_organoid_d1_S2 (refer to ocular organoid sample at day 1, replicate sample 2).
Libraries were prepared and sequenced in two experiments. One containing embryo samples embryo_d1, embryo_d2, embryo_d3, embryo_d4 and second containing embryo_d0 and organoid samples lens_organoid_d1, lens_organoid_d2, lens_organoid_d3 and lens_organoid_d4. To provide the internal reference and comparison of two experiments, RNA samples from embryo_d2_S2 and embryo_d4_S3 from the first experiment were used for library preparation and sequencing in second sequencing experiment (referred to as embryo_d2_S2_2 and embryo_d4_S3_2).
This dataset serves as supplemental information to the manuscript entitled Inverted Assembly of the Lens Within Ocular Organoids Reveals Alternate Paths to Ocular Morphogenesis. doi: https://doi.org/10.1101/2025.04.17.649366
Dataset: Advancing towards cancer theragnostic by probing the ²²⁵Ac decay chain with ultra-high-resolution metallic magnetic calorimeter based detectors
No full textThis dataset contains the raw, unprocessed measurement data underlying the results presented in the publication:
Advancing towards cancer theragnostic by probing the ²²⁵Ac decay chain with ultra-high-resolution metallic magnetic calorimeter based detectors
Files
The dataset is organized into two primary folders for the two measurement series:
2024_09-Tanzboden: Comparison measurements with ⁵⁵Fe
2024_09-Ac225: Measurements with ⁵⁵Fe and ²²⁵Ac, or ⁵⁵Fe, ²⁴¹Am, and ¹³³Ba
For each measurement series, the combined metadata (summary.csv, settings.csv, and log.csv) as well as the data for the first measurement are provided. The full dataset, totaling approximately 0.8 TB, is long-time archived at heiARCHIVE and is available upon request. A measurement contains the individual triggered signals recorded from the MMC-based detector array.
The processed spectra are not included here but are provided in the supplement dataset. For documentation on how to access, process, and analyze these raw measurements, please refer to the data analysis included in that supplement.
Data Structure
A measurement is a folder containing a Summary.txt file with general metadata, a Log.txt file with recording logs, and a subfolder for each ADC channel. Each ADC folder contains its configuration in ScopeSettings.txt as well as the folders POSP, BASE, and NEGP for positive, undefined, and negative polarity of the signals. Within the polarity folders, the signals are stored as *.sraw files inside *.zip archives.
Each line of a *.sraw signal is defined by a specific identifier. Lines starting with H contain general header information formatted as H [Key]: [Value]. The line starting with S marks the binary stream containing the raw signal samples. Lines starting with C contain comments, formatted similarly to the header as C [Number]: [String].
Contact
For questions regarding the dataset, or to request access to the full 0.8 TB raw data archive, please contact the corresponding authors of the publication.
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Psychotherapy Transcription Standards [data]
No full textThe datasets contains the newly developed psycholotheraupeutically informed transcription rules we developed within our study.
These transcription rules can be used to transcribe psychotherapeutic settings or can be implemented into AI.
We further provide the RMarkdown files for our four case studies. Due to restrictions by the ethical board commitee, the videografed psychotherapy sessions and questionnaires can not be shared. Thus, we provide the RMarkdown files which both contain our code and the corresponding results
Pediatric Snakebite in Sub-Saharan Africa: Clinical Predictors, Outcomes, and Gaps in Care – A Systematic Review [data]
No full textThis dataset contains study-level aggregated data underlying the systematic review “Pediatric Snakebite in Sub-Saharan Africa: Clinical Predictors, Outcomes, and Gaps in Care.” It includes extracted characteristics of included studies, quality assessment results, search strategies, antivenom use by country, clinical outcomes, prognostic indicators, and summary tables referenced as Supporting Information (S1–S6 Tables) in the manuscript. The data contain no individual patient information and are shared to support transparency, reproducibility, and reuse
Interstimulus-interval dependence in auditory cortex reflects perceptual organization of streams under informational masking [data]
No full textThe perceptual saliency of a regular sound sequence embedded into a well-chosen multi-tone-masker background fluctuates despite the absence of change in the physical stimulus. When such tone streams are perceived, each tone of the stream evokes a negative-going response referred to as awareness-related negativity (ARN). Here we probe if the ARN amplitude shows a dependence on the inter-stimulus interval (ISI) within perceived tone streams, similar to the N1 response evoked by unmasked tone streams independently of a task. Isochronous target streams were presented under multi-tone masking with ISIs of 500, 850, and 1200 ms, while listeners indicated when they perceived the onset of the target streams. A control experiment applied the same tone streams without masking in the presence of a second, narrow-band noise stream. In two separate runs, listeners either attended the tone streams and indicated their beginning and ending, or they attended the distractor streams, in which they performed a deviance detection task. Activity in auditory cortex was recorded with magnetoencephalography. Experiment 1 revealed a significant amplitude increment of the ARN with increasing ISI. In Experiment 2, the known amplitude dependence of the N1 was observed for attended and unattended runs alike. Critically, the negative difference waveform (Nd) between attended and unattended runs did not increase with the ISI. These results support an interpretation of the ARN as being related to the neural formation of an auditory stream and not as a pure reflection of attentional response enhancement