Biovelentia - Biological Research Journal
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POTENCY AND ACTIVITY OF SECONDARY METABOLITE OF Trichoderma harzianum AC1(b) J2 INHIBITOR GROWTH Colletotrichum capsici IPBCC 13.1098
No full textTrichoderma harzianum is a fungus that can produce secondary metabolites which able to inhibit the growth of Colletotrichum capsici. a pathogenic fungus causing anthracnose in plants. the Aims of this research were to obtain and analyze the ability of secondary metabolites of Trichoderma harzianum AC1 (b) J2 isolated from Scleria poaeformis. to inhibit the growth of Colletotrichum capsici IPBCC13.1098. The secondary metabolite eluat of Trichoderma harzianum were tested for the antifungal activity toward Colletotrichum capsici IPBCC 13.1098. Fractionation was carried out by Column Chromatography and produced 31 eluat. The secondary metabolite eluat of Trichoderma harzianum with the highest inhibition zone diameter was eluat 1 with 8.4 mm in diameter. The secondary metabolite eluat with the highest value of inhibition zone was carried out by MIC test and thin layer chromatography (TLC). Based on the MIC results, the minimum inhibitory concentration of the secondary metabolite of Trichoderma harzianum to fungus Colletotrichum capsici was 250 ppm with inhibition zone diameter was 0.10 mm. The results of TLC showed orange spots on the TLC plate that indicated alkaloite compounds
APPLICATION OF TRAP BARRIER SYSTEM COMBINED WITH CAGE TRAP FOR CONTROLLING RATS IN RICE FIELD
No full textRice-field rat is a main pest of rice. Yield reduction caused by rat is quite high, because rats attack rice plant at all stages. One of the efforts applied to control rats is by using Trap Barrier System combined with cage trap. The objective of this research was to identify species of rat attacking rice plants and investigate the presence of rat by their footprints. The experiment was conducted in Jalur 6 Village Sumber Mulya, Sub-district Muara Telang, District Banyuasin, South Sumatra. One hectare of farmer’s rice field, divided into 3 sub-plot. Each sub-plot was planted with variety paddy of Inpari 22 (sub-plot A), Inpari 33 (sub-plot B), and Inpari 43 (sub-plot C). The number of cage traps was 6 traps per sub-plot. Observations were made 7 times, with an interval of 10 days. Results showed species of trapped rats in the research area was Rattus argentiventer, in which number of males was higher than females. Morphologically, the size of males were bigger than female. Number of footprints did not reperesentative the number of trapped rats. Inpari 43 variety was more preferred by rats because damage intensity was the highest than other two rice varieties
DNA Extraction of Sumatran Striped Rabbit from Tissue Samples
No full textThe Sumatran Striped Rabbit (Nesolagus netscheri ) is a naturally rare, which is a protected animal based on Government Regulation Number 7 of 1999 and renewal of Permen LHK No. P.106 which are categorized as Data Deficient by the IUCN (International Union of Conservation of Nature) since 2019. Samples were obtained from Pagar Alam, South Sumatra. Most commonly found at elevations above 600 m in montane and sub-montane primary habitat. Molecular genetic characterization of sumatran striped rabbit is to know genetic information and genetic identification of N. netscheri. Primers 12S rRNA were used in this study to characterize Sumatran striped rabbit. A 1 band were detected ranged from 900 to 1000 bp. Molecular markers represent reliable tools which may have a great impact in rabbit breeding and genetic improvement of rabbits. Molecular markers on Nesolagus netscheri is expected to tools in the identification of Sumatran striped rabbits from South Sumatra
ANTIOXIDANT ACTIVITY of THE SECONDARY METABOLITES PRODUCED by ENDOPHYTIC FUNGI ISOLATED from JERUJU (Acanthus ilicifolius L.) PLANT
Get PDFTwelve endophytic fungi were isolated from Jeruju plant. Three of which have the best antioxidant activity, namely Culvularia lunata DJS1, Diaporthe phaseolorum DJS2, and Colletotrichum siamense BJS4. The fungi can produce secondary metabolites that have the potential as antioxidant compounds. This study aims to isolate the antioxidant compound using a chromatography column and test the antioxidant activity using DPPH method. Isolation of secondary metabolites produced 40 eluates. The IC50 value of DJS111 and BJS42 eluates were 10.20 µg/ml and 20.89 µg/ml, which is an antioxidant compound with very strong activity. The IC50 value of DJS24 eluate was 54.45 µg/ml, which is strong antioxidant activity. DJS111 eluate has antioxidant activity that approaches to ascorbic acid as a standart antioxidant compound (7.76µg/ml). From TLC, DJS111 contains flavonoid compound and DJS24 and BJS42 contain terpenoid compound
STUDY OF PRODUCTION AND VIABILITY COCOON OF Pontoscolex corethrurus FR.MULL AT VARIOUS CONCENTRATIONS OF CARBARYL INSECTICIDES
Get PDFResearch on the Study of Cocoon Production and Viability of Pontoscolex corethrurus Fr. Mull at various concentrations of Karbaril insecticide was carried out in July to October 2019, at the Laboratory of Animal Physiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Inderalaya, Ogan Ilir, South Sumatra. This study aims to study the production of cocoon and viabiltas earthworm Pontoscolex corethrurus Fr. Mull at various concentrations of carbaryl insecticide as well as getting concentrations of carbaryl insecticide began to react to the amount of cocoon and viability of the earthworm Pontoscolex corethrurus Fr.Mull. Contribution of research on the availability of data on the production of cocoon and viabiltas cocoon of earthworm Pontoscolex corethrurus Fr.Mull after being given various concentrations of carboxyl insecticide and providing information especially to farmers about the effect of various concentrations of carbaryl insecticide on non-target animals, especially soil animals namely Pontoscolex corethrurus Frethrurus earthworm . Mull. This study uses a completely randomized design with 6 (six) treatments and 5 (five) repetitions. The treatment given in the form of carboxyl insecticide with a concentration of 0% (control); 0.1%; 0.2%; 0.3%; 0.4%; 0.5%. Data were analyzed by Analysis of Variance, if there were significant differences followed by Duncan's Test at a 95% confidence level. The results showed that the treatment of various concentrations of carboxyl insecticide significantly affected the average number of cocoon and viability of the earthworm cocoon Pontoscolex corethrurus Fr. Mull. Average number of earthworm cocoons Pontoscolex corethrurus Fr. The lowest Mull was found in the administration of carboxyl insecticide at a concentration of 0.5%, 0.62 ± 0.06 cocoon / individual / week and the average number of earthworm cocoons Pontoscolex corethrurus Fr. The highest Mull was found in the administration of carboxyl insecticide at a concentration of 0% (control), namely 3.62 ± 0.02 cocoon / individual / week. The average viabiltas cocoon of earthworms Pontoscolex corethrurus Fr. The lowest Mull was found in the administration of carboxyl insecticide at a concentration of 0.5%, namely 18.85 ± 1.19% and the average viability of the earthworm cocoon Pontoscolex corethrurus Fr. The highest Mull was found in the administration of carboxyl insecticide at a concentration of 0%, namely 98.65 ± 2.15
FOOD HABITS OF LUNDU FISH (Mystus gulio Hamilton, 1822) IN THE WATERS OF SUNGAI DUA (TWO RIVERS) VILLAGE, RAMBUTAN DISTRICT, BANYUASIN REGENCY, SOUTH SUMATERA
No full textThe research about food habits of Lundu fish (Mystus gulio Hamilton, 1822) in the waters of Sungai Dua (Two Rivers) Village, Rambutan District, Banyuasin Regency, South Sumatra. The sampling was conducted in five times in December 2016; January, February, March and April 2017. Identification of the samples was in Laboratory of Animal Taxonomy, Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indralaya.The purpose of the research was to analyze the food habits of Mystus gulio Hamilton, determine the relationship of length-weight, determine the conditions, analyze the gastric fullness index. The results of this research showed that the food habits of Mystus gulio Hamilton is plankton, like Cyanophyta, Chlorophyta, Diatomae, Desmidiacae, Euglenophyta, Pyrrophyta, Ciliata, Rhizopoda, Rotaria, and Entomostraca
RECENT OBSERVATIONS OF Elephas maximus sumatranus IN SEMBILANG NATIONAL PARK, SOUTH SUMATRA
No full textSumatran elephants (Elephas maximus sumatranus) are one of four Asian elephants subspecies, which are categorized as Critically Endangered by the IUCN (International Union of Conservation of Nature) since 2011. Some factors that might drive this species into extinction ranges from wild attacks in the forest, habitat degradation due to land acquisition for plantation and development areas, and human slaughter because they regard these animals as enemies that sometimes enter community settlements. Elephants in Sembilang National Park is an interesting issue because it has not been registered as key species in Sembilang National Park, as well as no authentic evidence and official reports about the existence of elephants in the park. This paper proves the existence of elephants in Sembilang National Park, South Sumatra. The elephant documentation is in the form of photos and videos of 4 different elephant individuals, footage, cruising lanes and elephant puddles. Evidence of the existence of elephants in Sembilang National Park strongly indicates that future actions, participation and attention of all stakeholders are needed to the conservation efforts of the biggest Sumatran mammals in Sembilang National Par
EXPLORATION OF ENDOPHYTIC FUNGI OF DRAGON SCALE’S FERN (Pyrrosia piloselloides (L.) M.G. Price) AS AN ANTIBACTERIAL SOURCES
Get PDFEndophytic fungi are fungi which live inside the host plant tissue and have been undergone a horizontal gene transfer process. Endophytic fungi are able to synthesize the same bioactive compounds which synthesized by their host plants. The host plant used in this research was dragon’s scales fern (Pyrrosia piloselloides (L.) M.G. Price). Dragon’s scales fern produces various of bioactive compounds which used as antibacterial agents such as polyphenols. This research was aimed to obtain endophytic fungi isolates from trophophyll fronds and sporophyll fronds of dragon’s scales fern, to determine the antibacterial activity of the secondary metabolite extracts of endophytic fungi, to determine the Minimum Inhibitory Concentration (MIC), to determine the characteristics of the endophytic fungi isolates which potentially as antibacterial source. Based on the research, 13 endophytic fungi isolates were obtained from dragon’s scales fern fronds consist of 5 isolates from trophophyll fronds and 8 isolates from sporophyll fronds. The antibacterial activity test showed that the extract of secondary metabolites of the isolate DTP2 had the highest inhibition zone diameter against E.coli 14.82 ± 4.05 mm, DTP4 against S.aureus 8.80 ± 0.03 mm and DSP4 against S.dysentriae 10.15 ± 0.36 mm. MIC of ethyl acetate extracts of secondary metabolites of isolate DTP2 against E.coli was 125 µg/mL, DTP4 against S.aureus was 125 µg/mL and DSP4 against S.dysentriae was 31.25 µg/mL. The endophytic fungi isolate DTP2 identified as Aureobasidium melanogenum, DTP4 identified as Penicillium alliisativi and DSP4 identified as Aspergillus flocculosus
RECENT OBSERVATION OF FALSE GHARIAL (Tomistoma schlegelii) IN THE SEMBILANG NATONAL PARK (TNS) AREA, BANYUASIN REGENCY, SOUTH SUMATERA PROVINCE
No full textA research about False Gharial (Tomistoma schlegelii) which is a protected animal based on National Government Regulation Number 7 of 1999 and renewal of Permen LHK No. P92/MENLHK/SETJEN/KUM.1/8/2018 has been conducted on 15 until 19 September 2017 located in the region of SPTN 3 Hulu Benu River Muara Merang Village 3 Pancuran Village, and on 27 February until 04 March 2018 located in the region of SPTN 3 area Hulu Benu River Tanah Pilih Village, Berbak Sembilang National Park (TNBS), South Sumatra. This reaserch aims to monitoring the conditions of habitat and its populations for the purpose of conservation and management of TNBS areas to be more optimal. The methods used is direct monitoring during the day and night, indirect monitoring through information and interviews with citizens, as well as secondary data collection. The results obtained, were that there was an encounter and information about the existence of False Gharials measuring ± 1 to 5 meters in the upper reaches of the Benu River in the Merang River, Rasau River, Bagan 2, Bagan 6 through Bagan 9. This resulted in the determination of the Benu River TNBS as an important habitat False Gharials that needs to be managed appropriately as conservation area
EFECT OF LEAF FERTILIZER ON SECOND TREATMENT TO THREE GENOTYPES CORN EFFICIENT CROPS IN TIDAL LAND
Get PDFThis study aims to determine the effect of leaf fertilizer on three nutrient efficient corn genotypes in tidal land. This research was coducted in Village Mulia sari, Sub-district Tanjung Lago, District Banyuasin, South Sumatra in the noble village of Sari Tanjung Lago sub-district from August to December 2017. This study uses the Split Plot Design consisting of 3 factors, namely the first factor of genotype, the second factor is the dose of urea A0 = 0 kg, A1 = 250 kg, A2 = 300 kg and the third factor of leaf fertilizer consists of: P0 = (control without spraying), P1 = 30 days of spray, P2 = 45 days of spray, P3 = 30 + 45 days of spray, P4 = 60 days of spray, P5 = 75 days of spray, P6 = 60 + 75 days of spray. repeated three times. Observation results used of 300 kg of urea fertilizer and leaf fertilizer had a good influence on the growth of G3 corn genotypes and the production of corn plants in tidal land. The combination of giving 300 kg of urea fertilizer + leaf fertilizer gives a pretty good influence on the growth of G3 corn genotypes with a production of 7.44 Ton/hectare